Anti-FLAG (M5)-SAP [IT-59, KIT-59]

a tool for eliminating FLAG (M5)-expressing cells; targeted via mouse monoclonal antibody to N-terminal Met-FLAG fusion protein, eliminated via saporin

SKU: IT-59 Category: Quantity: Individual 25 ug, Individual 100 ug, Individual 250 ug, Individual 1 mg, Kit w/controls 25 ug, Kit w/controls 100 ug, Kit w/controls 250 ug | Antibody Type: Monoclonal | Host: mouse | Conjugate: saporin | Usage: eliminates cells |

A fusion tag called FLAG consists of eight amino acids (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys). FLAG is specifically designed for immunoaffinity chromatography. It allows elution under non-denaturing conditions. Several antibodies against this peptide have been developed. One antibody denoted as M5 detects N-terminal Met-FLAG fusion proteins.

Anti-FLAG (M5)-SAP is a chemical conjugate of a mouse monoclonal antibody to the N-terminal Met-FLAG fusion protein and the ribosome-inactivating protein, saporin. It specifically eliminates cells with extracellular expression of FLAG-tagged proteins and cells that recognize YOUR FLAG-tagged recombinant proteins.

Anti-FLAG (M5)-SAP is available individually (Cat. #IT-59) or as a kit (Cat. #KIT-59) which includes Anti-FLAG (M5)-SAP and Mouse IgG-SAP (Cat. #IT-18).

keywords: fusion tag, FLAG, Met-FLAG, M5, N-terminal, Anti-FLAG, Anti-M5, FLAG-tagged, saporin, secondary


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View Data Sheet

Saporin (Cat. #PR-01)

Mouse IgG-SAP (Cat. #IT-18)

Tag-Targeted Toxins

Cytotoxicity Assay for Targeted Toxins in vitro

Concentration Calculation Explained: Convert molarity to mg/ml and mg/ml to molarity

Preparing and Interpreting Cytotoxicity Data in vitro

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