A fusion tag called FLAG consists of eight amino acids (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys). FLAG is specifically designed for immunoaffinity chromatography. It allows elution under non-denaturing conditions. Several antibodies against this peptide have been developed. One antibody denoted as M5 detects N-terminal Met-FLAG fusion proteins.
Anti-FLAG (M5)-SAP is a chemical conjugate of a mouse monoclonal antibody to the N-terminal Met-FLAG fusion protein and the ribosome-inactivating protein, saporin. It specifically eliminates cells with extracellular expression of FLAG-tagged proteins and cells that recognize YOUR FLAG-tagged recombinant proteins.
Anti-FLAG (M5)-SAP is available individually (Cat. #IT-59) or as a kit (Cat. #KIT-59) which includes Anti-FLAG (M5)-SAP and Mouse IgG-SAP (Cat. #IT-18).
keywords: fusion tag, FLAG, Met-FLAG, M5, N-terminal, Anti-FLAG, Anti-M5, FLAG-tagged, saporin, secondary
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