tt2012

Targeting Trends 2012 Newsletter

Read the 2012 Newsletters 2012, issue 1:  Motivation’s modulation of attention through the mesolimbic-corticopetal cholinergic circuitry / by M St. Peters, M Sarter / featuring 192-IgG-SAP (Cat. #IT-01), Mouse IgG-SAP (Cat. #IT-18) SFN Poster of the Year Targeting Talk: Anti-NGFr blocking antibody, Staining with FITC-labeled Anti-Saporin Targeting Tools: Antibody to Metabotropic Glutamate Receptor 2, Tag-Targeted Toxins …

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Targeting Tools: mu p75-SAP

The cover article this quarter demonstrates usage of mu p75-SAP (Cat. #IT-16) to study delirium. To create this toxin, we affinity-purified the rabbit polyclonal to the low affinity neurotrophin receptor, p75, (Cat. #AB-N01AP) with the immunogen bound to a solid support, and then conjugated the affinity-purified antibody to saporin (Cat. #PR-01). As can be seen …

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Targeting Topics 12q4

Control of sleep and wakefulness. Brown RE, Basheer R, McKenna JT, Strecker RE, McCarley RW. Physiol Rev 92(3):1087-1187, 2012. This review summarizes mechanisms in the brain that control sleep and wakefulness. Areas discussed include wakefulness promoting systems, non-REM sleep and REM sleep definitions, the function of each kind of sleep, and dysfunction that occurs as …

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Cover Article: Partial basal forebrain cholinergic depletion leaves working memory susceptible to the effects of systemic inflammation

Contributed by Dr. Colm Cunningham, Trinity College, Institute of Neuroscience & School of Biochemistry and Immunology, Dublin, Ireland It is well established that peripheral inflammation can signal the intact CNS to bring about adaptive changes in behavior during the sickness response. However, during aging and dementia, the brain is particularly susceptible to the deleterious effects …

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Targeting Tools: Anti-SERT-SAP

Anti-SERT-SAP (Cat. #IT-23) utilizes a monoclonal antibody to the fourth extracellular domain of the serotonin re-uptake transporter (SERT). SERT is the major determinant of serotonin inactivation following release at synapses, is the site of action for many tricyclic antidepressants and the SSRIs (serotonin-selective reuptake inhibitors), and is also targeted by a number of psychostimulants including …

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Targeting Tools: Mac-1-SAP

The Mac-1-SAP targeted toxins recognize Mac-1-positive (CD11b) cells in mouse/human (Cat. #IT-06) or in rat (Cat. #IT-33). The toxin is excellent for removing contaminating macrophages from primary cultures. Elimination of macrophages in vivo is useful to determine their role(s) in autoimmune diseases and in degenerative diseases such as Alzheimer’s. Both toxins are extremely potent, with …

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Targeting Tools: Anti-GST

The Glutathione S-Transferase (GST) family of enzymes contains cytosolic, mitochondrial, and microsomal proteins that are capable of multiple reactions with a multitude of substrates, both endogenous and xenobiotic. GST catalyzes the conjugation of reduced glutathione which is useful in the detoxification of endogenous compounds such as peroxidized lipids, as well as the metabolism of xenobiotics. …

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Targeting Topics 12q3

C1 neurons excite locus coeruleus and A5 noradrenergic neurons along with sympathetic outflow in rats. Abbott SB, Kanbar R, Bochorishvili G, Coates MB, Stornetta RL, Guyenet PG. J Physiol 590(12):2897-2915, 2012. C1 neurons are known to activate sympathetic tone and stimulate the hypothalamic-pituitary-adrenal axis. C1 activation is also reported to inhibit locus coeruleus (LC) neurons. …

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Cover Article: A pivotal role of lumbar spinothalamic cells in regulation of ejaculation via intraspinal connections

Contributed by Lique M. Coolen, University of Michigan Introduction from Dr. Lappi: An article by Lique Coolen’s group[5] was the 1000th article in PubMed in response to the search word ‘saporin.’ Dr. Coolen has graciously agreed to describe the latest in her fascinating work. Male sexual behavior consists of many different aspects, with ejaculation being …

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Targeting Tools: Anti-RFP

Red fluorescent protein (RFP) is derived from Discosoma sea anemone. This antibody (Anti-RFP, Cat. #AB-332) recognizes native and denatured forms of RFP. The antigen for this antibody is present in all of these fluorescent molecules: RFP, tag-RFP, turbo-RFP, DeRed, mCherry, and mOrange. The epitope for the antibody is part of the RFP N-terminal sequence. RFP …

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