Targeting Trends 2010 Newsletter
Read the 2010 Newsletters and recent scientific references 2010, issue 1: 2010, issue 2: 2010, issue 3: 2010, issue 4:
Targeting Trends 2010 Newsletter Read More »
tt2010
Targeting Tools: GAT1-SAP
GAT-1 is a sodium-coupled neurotransmitter transporter responsible for moving g-aminobutyric acid (GABA) across cell membranes. GABA is the predominant inhibitory neurotransmitter in the mammalian central nervous system. GAT-1 is widely distributed in both the central and peripheral nervous systems. GAT-1 and GABA are present in numerous neuronal pathways, some of which are implicated in epilepsy,
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Cover Article: Targeted Ablation of Sympathetic Neurons Reduces Ventricular Arrhythmias and Autonomic Dysreflexia
Contributed by Heidi L. Lujan and Stephen E. DiCarloDepartment of Physiology, Wayne State University School of Medicine, Detroit, MI 48201 Excessive sympathetic activity is responsible for, and/or contributes to, the morbidity and mortality associated with cardiovascular diseases (e.g. hypertension, stroke, heart failure, ischemic heart disease, ventricular arrhythmias). For example, myocardial ischemia provokes a powerful reflex
Targeting Tools: Targeting NGF Receptor-Positive Neurons
Targeting in mouse: mu p75-SAP (Cat. #IT-16) To create this targeted toxin, we affinity-purified the rabbit polyclonal with the immunogen bound to a solid support, and conjugated the affinity-purified antibody (Cat. #AB-N01AP) to saporin. As can be seen in the cytotoxicity assay below, mu p75-SAP has an EC50 in the picomolar range. This greater potency
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Targeting Tools: CTB-SAP
CTB-SAP (Cat. #IT-14) is a conjugate between the cell-binding component of cholera toxin (the B chain) and saporin. CTB binds to GM1 (monosialotetrahexosylganglioside), which is present on the surface of different neurons. It has been suggested to be involved in many problems (besides the most famous in the gut: cholera) of neuronal systems: Parkinson’s, motor
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Targeting Article: Poster at American Association for Cancer Research Meeting
At this year’s AACR meeting, Leonardo Ancheta, scientist and Product Manager from ATS, presented a poster on our new monoclonal antibody to Basigin-2 (Cat. #AB-42). The poster describes our work with the antibody and the immunotoxin made from it (Cat. #IT-54). Basigin-2 (EMMPRIN, CD147) is widely expressed on the surface of numerous tumor types and
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Cover Article: Role of Cell Fate Determinants in a Model of Spinal Cord Neurotoxic Lesion Induced by Cholera Toxin B-Saporin
Contributed by Rosario Gulino, Vincenzo Perciavalle and Massimo Gulisan Dept of Physiological Sciences, Univ of Catania, Viale Andrea Doria, 6-I95125 Catania ITALY A promising approach for central nervous system (CNS) repair consists in the activation of endogenous neural precursor cells (NPCs), but this process is less efficient in the spinal cord (SC) following a spinal
Targeting Tools: Fab-ZAPs
New Secondary Conjugates – Fab-ZAPs – Use Monovalent Secondary Antibodies Linked to Saporin For the past decade scientists have extensively used secondary conjugates (e.g. Mab-ZAP; Cat. #IT-04, and Hum-ZAP; Cat. #IT-22) to make their own targeted toxins for in vitro use. The ability to combine: 1) a primary antibody to a cell-surface marker with 2)
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Targeting Tools: Anti-6 His-SAP
Anti-6 His-SAP (Cat. #IT-52) Composed of an antibody to 6 His conjugated to saporin, this secondary conjugate should be used as a diagnostic tool for testing your primary, 6 His-tagged proteins for specific cell surface epitope binding and internalization. The 6 His tag is widely used because of its affinity to bind nickel or cobalt
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Targeting Tools: Anti-GFP-SAP
Anti-GFP-SAP (Cat. #IT-53) The green fluorescent protein (GFP) gene can be introduced and maintained in the genome through breeding, injection with a viral vector, or cell transformation. Whether your cells express a surface protein with a GFP-coded region, or you have a protein that targets specific cells and contains a GFP tag, Anti-GFP-ZAP can be
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