tt2009

Targeting Trends 2009 Newsletter

Read the 2009 Newsletters 2009, issue 1: Selective deletion of CD8+ T cells by saporin-coupled MHC class I tetramers / by Hess PR, Buntzman AS, Murray SL, Young EF, Frelinger JA / featuring Streptavidin-ZAP (Cat. #IT-27), FITC-labeled SAP antibody (Cat. #FL-02) SFN Poster of the Year Targeting Talk: IgM Primary Antibody Assay 2009, issue 2: Deletion of Catecholaminergic Neurons …

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Targeting Tools: Oxytocin-SAP

Oxytocin-SAP (Cat. #IT-46) is a conjugate (molecular weight 31 kDa) between the native oxytocin peptide and saporin. Oxytocin is a nine amino acid peptide with a structure similar to vasopressin, and is a hormone released by the posterior pituitary. Peripheral activity of oxytocin is linked primarily to lactation and labor-related uterine contractions. Within the brain, …

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Targeting Tools: Anti-Basigin

This mouse monoclonal antibody (Cat. #AB-42; Clone P2C2) recognizes human Basigin. The antibody is purified by ammonium sulfate precipitation on conditioned medium and then further purified through Protein-A. The antibody is routinely tested by flow cytometry. The immunoglobulin superfamily protein basigin (EMMPRIN/CD147) is a cell surface glycoprotein expressed by tumor cells that stimulates matrix metalloproteinase …

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Targeting Tools: Bombesin-SAP

Bombesin-SAP (Cat. #IT-40) specifically targets and eliminates gastrin- releasing peptide receptor (GRPR)-positive cells. This targeted toxin (molecular weight 31.8 kDa) is a chemical conjugate of bombesin and the ribosome-inactivating protein, saporin. Please see cover article (pp. 1, 6) for an example of its experimental use.

Targeting Topics 09q4

Evaluation of side effects through selective ablation of the mu opioid receptor expressing descending nociceptive facilitatory neurons in the rostral ventromedial medulla with dermorphin-saporin Cao F, Chen SS, Yan XF, Xiao XP, Liu XJ, Yang SB, Xu AJ, Gao F, Yang H, Chen ZJ, Tian YK Neurotoxicology 30(6):1096-1106, 2009. Selective ablation of rostral ventromedial (RVM) …

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Cover Article: Ablation of GRPR+ Neurons in the Spinal Cord by Bombesin-Saporin Knocks Out Itch Sensation in Mice Without Affecting Pain Circuit

Contributed by Zhou-Feng Chen, Yang-Gang Sun, Zhong-Qiu Zhao, Xiu-Li Meng, Jun Yin, Xian-Yu Liu; Washington University Pain Center School of Medicine, Dept of Anesthesiology, St. Louis, MO 63141 Unlike pain, itch sensation evokes scratching response instead of withdrawal behavior. However, itch and pain sensations share many similarities. Like chronic pain, chronic itch is a major clinical …

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Targeting Topics 09q3

Photostimulation of retrotrapezoid nucleus phox2b-expressing neurons in vivo produces long-lasting activation of breathing in rats Abbott SB, Stornetta RL, Fortuna MG, Depuy SD, West GH, Harris TE, Guyenet PG J Neurosci 29(18):5806-5819, 2009. The retrotrapezoid nucleus (RTN) contains a subpopulation of cells that are thought to function as central respiratory chemoreceptors. The authors used bilateral …

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Targeting Article: SBIR Grant Funded: Selective Activation in Neuronal Populations

ATS has been awarded a Phase I SBIR grant to develop a new line of products. Dr. Douglas Lappi is the Principal Investigator and Brian Russell is the Lead Scientist on the project. Molecules targeted towards cell surface markers have been used for years to identify specific cell types. It has been demonstrated over the …

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Cover Article: Depletion of Microglia by Mac-1-SAP in Mouse Hippocampal Slice Cultures Enhances Ischemia-Like Neurodegeneration

Contributed by Maria Montero (a), Berta Gonzalez (b), Jens Zimmer (a)(a) Anatomy and Neurobiology, University of Southern Denmark, Denmark(b) Histology Unit, Autonomous University of Barcelona, Spain Microglial cells contribute about 12% of the cells in the brain, acting as “biosensors” for homeostatic regulation in normal and pathological conditions.[3,13] Resting microglial cells in the adult brain …

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Targeting Tools: CTB-SAP

CTB-SAP (Cat. #IT-14) is a conjugate between the cell-binding component of cholera toxin (the B chain) and saporin. CTB binds to GM1 (monosialotetrahexosylganglioside), which is present on the surface of different neurons. It has been suggested to be involved in many problems (besides the most famous in the gut: cholera) of neuronal systems: Parkinson’s, motor …

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