tt2008

Targeting Trends 2008 Newsletter

Read the 2008 Newsletters 2008, issue 1: Role of medial septal GABAergic neurons in learning and extinction: Effects of the novel GABA immunotoxin GAT1-SAP / by Pang KCH, Jiao X, Servatius RJ / featuring GAT1-SAP (Cat. #IT-32) SFN Poster of the Year Targeting Talk: Secondary Conjugate Protocol, Reducing Agents Targeting Tools: New Products Stimulate Instead …

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Targeting Tools: Anti-Saporin

Saporin is a plant enzyme with N-glycosidase activity that depurinates a specific nucleotide in the ribosomal RNA 28S, thus irreversibly blocking protein synthesis. Affinity-purified Anti-Saporin rabbit polyclonal (Cat. #AB-41AP) is the most recent addition to our line of Saporin antibodies. These antibodies can be used as controls with our Saporin-conjugate immunotoxins, for immunoblotting, and in …

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Targeting Topics 08q4

Tomoregulin internalization confers selective cytotoxicity of immunotoxins on prostate cancer cells. Zhao XY, Liu HL, Liu B, Willuda J, Siemeister G, Mahmoudi M, Dinter H Transl Oncol 1:102-109, 2008. Tomoregulin is a type 1 transmembrane protein with a short cytoplasmic tail, and is found in the brain and prostate. After confirming cell surface localization by …

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Targeting Article: Control Circadian Rhythm?

Advanced Targeting Systems announces the release of Melanopsin-SAP (Cat. #IT-44), a reagent that specifically eliminates intrinsically photosensitive retinal ganglion cells (ipRGCs) that contain melanopsin. This material is described in Göz et al.[1] and consists of an antibody to an extracellular domain conjugated to saporin. The ipRGCs, with their amazing long processes, have become major players …

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Cover Article: Selective lesions of amygdala intercalated neurons using the Dermorphin-SAP immunotoxin reveal their role in extinction of conditioned fear.

Contributed by Ekaterina Likhtik Columbia University,New York Psychiatric Institute, 1051 Riverside Drive, New York, NY 10032 Introduction by Douglas Lappi, Ph.D., President/CSO: This quarter’s Cover Article is from Ekaterina Likhtik who reprises her recently published work in Nature 454:642-645. In the News and Views, Sah and Westbrook comment that “Neuronal receptors in these circuits—such as …

Cover Article: Selective lesions of amygdala intercalated neurons using the Dermorphin-SAP immunotoxin reveal their role in extinction of conditioned fear. Read More »

Targeting Topics 08q3

Noradrenergic innervation of the dorsal medial prefrontal cortex modulates hypothalamo-pituitary-adrenal responses to acute emotional stress. Radley JJ, Williams B, Sawchenko PE J Neurosci 28:5806-5816, 2008. Rats were injected with 90-120 nl of 0.475-µg/µl anti-DBH-SAP (Cat. #IT-03) into the cortical field containing noradrenergic neurons that project to the dorsal medial prefrontal cortex. The results indicate that …

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Cover Article: Noradrenergic Innervation of the Dorsal Medial Prefrontal Cortex Modulates Hypothalamo-Pituitary-Adrenal Responses to Acute Emotional Stress

Contributed by Jason J. Radley, Ph.D., Laboratory of Neuronal Structure and FunctionThe Salk Institute for Biological Studies and Foundation for Medical Research, La Jolla, CA 92037, USA The medial prefrontal cortex (mPFC) has been proposed to play a role in the inhibition of hypothalamo-pituitary-adrenal (HPA) responses to emotional stress via influences on neuroendocrine effector mechanisms …

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Targeting Tools: mu p75-SAP

In 2004, ATS re-designed the anti-murine p75-SAP targeted toxin (mu p75-SAP, Cat. #IT-16) and produced a conjugate that is much more potent in our in vitro cell cytotoxicity assays. Previously, we used a rat monoclonal antibody. This antibody had been outperformed by our rabbit polyclonal (Cat. #AB- N01), in several assays, especially flow cytometry analysis …

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Targeting Topics 08q2

Septal grafts restore cognitive abilities and amyloid precursor protein metabolism. Aztiria E, Cataudella T, Spampinato S, Leanza G. Neurobiol Aging 30(10):1614-1625, 2009. Although cholinergic loss and the presence of b-amyloid plaques are well documented in Alzheimer’s disease, it is unknown whether restoration of regulatory cholinergic inputs affects in vivo b-amyloid precursor protein (APP). 5 µg …

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