Streptavidin-ZAP [IT-27]

a tool to “piggyback” onto YOUR biotinylated material via streptavidin; targeting cells that recognize YOUR biotinylated material, eliminated via saporin

SKU: IT-27 Category: Quantity: Individual 25 ug, Individual 100 ug, Individual 250 ug, Individual 1 mg | Conjugate: saporin | Usage: converts biotinylated materials, eliminates cells |

Streptavidin-ZAP converts biotinylated materials into targeted toxins. Streptavidin is a tetrameric protein (molecular weight 53 kDa in its recombinant form), with each subunit able to bind a single biotin molecule. The bond between streptavidin and biotin is rapid and essentially non-reversible, unaffected by most extremes of pH, organic solvents, and denaturing reagents. It is the strongest known noncovalent biological interaction (Ka = 1015 M-1) between protein and ligand. The streptavidin used to make Streptavidin-ZAP contains no carbohydrate group and has a neutral isoelectric point, which therefore reduces the nonspecific binding as compared to avidin. A variety of molecules, including lectins, proteins, and antibodies, can be biotinylated and reacted with streptavidin-labeled probes or other detection reagents for use in biological assays.

Streptavidin-ZAP is a chemical conjugate of streptavidin and the ribosome-inactivating protein, saporin. It converts biotinylated materials into targeted toxins.

These kits convert biotinylated materials into targeted toxins and will allow you to evaluate the ability of your reagent to internalize upon binding to its receptor.

TargetRecommended Product
in vivo
Recommended Product
in vitro
biotinylated peptideStreptavidin-ZAP Peptide Kit
(KIT-27-B)
Biotin-Z Peptide Internalization Kit
(KIT-27-ZB)
biotinylated antibody
*choose host species of your material
Streptavidin-ZAP Antibody Kit
(KIT-27-A)
goat, human, mouse, rat, or rabbit
Biotin-Z Antibody Internalization Kit
(KIT-27-Z)
goat, human, mouse, rat, or rabbit
other biotinylated materialStreptavidin-ZAP Kit
(KIT-27-S)
Biotin-Z Internalization Kit 
(KIT-27-ZS)

Other ZAP Conjugates:

Need another ZAP Conjugate for your target? Check here for other species & targets including in vivo and in vitro options.

keywords: Streptavidin, Avidin, Biotin, screening, ADC, Antibody Drug Conjugate, in vivo, saporin, streptavidinylated, Streptavidin-SAP, Streptavidin-ZAP, Strep-ZAP, Strep-SAP

Reviews

  1. sisi.zheng

    Technical support was extremely valuable in ensuring we ordered the right products that suited our research needs. The website also contains multiple useful protocols and online calculators to determine how much of the streptavidin-ZAP to incubate with our biotinylated antibody. Order arrived promptly and well packaged.

  2. annie.gagnon

    Streptavidin-ZAP is routinely tested by cytotoxicity assay. It is formatted in PBS solution without preservative, and it is sterile. There is no need to do further purifications. So, it is very convenient for downstream cell killing experiments both in vitro and in vivo. This product is also time-saving. Just incubate your biotinylated material with Streptavidin-ZAP for 15min at room temperature, then it’s ready for use. In addition, biotin and streptavidin has the strongest known noncovalent biological interaction. It is essentially nonreversible. Don’t worry about the dissociation. Saporin protein can effectively target and kill the cell with your specific biotinylated material once they are internalized by the cell.

  3. ahmed.jalal

    Worked seamlessly. Technical support was excellent.

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View Data Sheet

Streptavidin-saporin: Converting biotinylated materials into targeted toxins

Ancheta LR, Shramm PA, Bouajram R, Higgins D, Lappi DA (2023) Streptavidin-saporin: Converting biotinylated materials into targeted toxins. Toxins 15(3):181. doi: 10.3390/toxins15030181

Summary: This manuscript describes the myriad of ways Streptavidin-ZAP is used and how this technology supports the scientific process of ‘Molecular Surgery’ and progress in research and drug development. Insights from publications and research performed using Streptavidin-ZAP and its impact on academia and industry for research and drug development are presented.

Read the full article.

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Saporin as a commercial reagent: its uses and unexpected impacts in the biological sciences-tools from the plant kingdom

Ancheta LR, Shramm PA, Bouajram R, Higgins D, Lappi DA (2022) Saporin as a commercial reagent: its uses and unexpected impacts in the biological sciences-tools from the plant kingdom. Toxins (Basel) 14(3):184. doi: 10.3390/toxins14030184

Read complete article.

Antibody drug conjugates targeted to CD45 or CD117 enable allogeneic hematopoietic stem cell transplantation in animal models

Palchaudhuri R, Hyzy SL, Proctor JL, Adams HL, Pearse BR, Sarma G, Aslanian S, Gillard G, Lamothe TL, Burenkova O, Brooks ML, Gabros AD, McDonagh CF, Boitano AE, Cooke MP (2018) Antibody drug conjugates targeted to CD45 or CD117 enable allogeneic hematopoietic stem cell transplantation in animal models. Blood 132:3324. doi: 10.1182/blood-2018-99-119432

Objective: To further investigate and develop the utility of CD45-SAP and CD117-SAP, in combination with immunosuppression, in murine transplant models using i.v. administration in an allogeneic minor mismatch transplant model (Balb/c donor into DBA/2 recipients).

Summary: CD45-SAP or CD117-SAP in combination with immunosuppressants (30Fll and post-transplant Cytoxan) enabled >85% peripheral donor chimerism at 12 weeks post-transplantation. CD45-SAP and CD117-SAP were more effective at conditioning versus 2Gy TBI or pretransplant Cytoxan.

Usage: CD45-SAP (1.9 mg/kg, iv) and CD117-SAP (1mg/kg, iv) in an allogeneic minor mismatch transplant model (Balb/c donor into DBA/2 recipients).

Related Products: Streptavidin-ZAP (Cat. #IT-27)

Antibody-based preparative regimens for cell, tissue and organ transplantation

Van Hentenryck M, Li Z, Murphy PM, Czechowicz A (2022) Antibody-based preparative regimens for cell, tissue and organ transplantation. (eds. 162). OBM Transplantation 6(3):162. doi: 10.21926/obm.transplant.2203162

Objective: Provide a review of progress in the use of antibodies to support cell and tissue transplantation with a particular focus on induction of donor-specific tolerance for solid organ transplantation.

Summary: Antibody-based conditioning to prepare the recipient is a promising approach towards achieving transplant tolerance in both hematopoietic and solid organ transplant settings.

Usage: To enhance HSC depletion while avoiding bystander toxicity (neutropenia, lymphopenia, and thrombocytopenia) caused by CD45-radioimmunotherapy, Palchaudhuri et al. developed a saporin-based CD45 (CD45-SAP) immunotoxin using a biotinylated antibody and Streptavidin-ZAP.

Related Products: Streptavidin-ZAP (Cat. #IT-27)

See Also:

Evaluate Potential Targeting Molecules.

Kohls M (2006) Evaluate Potential Targeting Molecules. Nature Methods

Summary: Targeted toxins -- targeting agents conjugated to saporin -- are widely used to eliminate specific cell populations both in vitro and in vivo. For these molecules to be effective, it is vital that the targeting component of the conjugate specifically binds the cells of interest. A secondary conjugate, Streptavidin-ZAP, has been created by attaching the toxin saporin to streptavidin. The user can combine primary biotinylated material with Streptavidin-ZAP to quickly and economically screen potential targeting molecules for internalization and specificity. Once the appropriate targeting molecule is identified, a direct conjugation with saporin can be performed.

Related Products: Streptavidin-ZAP (Cat. #IT-27)

Read the article.

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Streptavidin-ZAP (Cat. #IT-27)

Streptavidin-ZAP Antibody Kit, in vivo (KIT-27-A)

Streptavidin-ZAP Peptide Kit, in vivo (KIT-27-B)

Streptavidin-ZAP Kit, in vivo or in vitro (KIT-27-S)

Biotin-Z Antibody Internalization Kit, in vitro (KIT-27-Z)

Biotin-Z Peptide Internalization Kit, in vitro (KIT-27-ZB)

Biotin-Z Internalization Kit, in vitro (KIT-27-ZS)

FITC-Streptavidin-ZAP (Cat. #IT-85)

MonoBiotin-ZAP (Cat. #BT-ZAP)

Streptavidin-pHast (Cat. #PH-03)

MonoBiotin-ZAP (Cat. #BT-ZAP)

Streptavidin-ZAP Concentration 

Equimolar Mixing of Strep-ZAP and Biotinylated Molecule

DMSO% for peptide and Streptavidin-ZAP

Streptavidin-ZAP concentration ratio

Biotin-Z Internalization Kit Introduction

Biotin-Z Internalization Kit Tutorial

Biotin-Z (Streptavidin-ZAP) Kit Literature

Cytotoxicity Assay Protocol for ZAP Biotin-Z Internalization Kits

Online Calculator: Preparing ZAP Biotin-Z Kit Samples

Concentration Calculations: Convert molarity to mg/ml and mg/ml to molarity

Preparing and Interpreting Cytotoxicity Data in vitro


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