Choline acetyltransferase (ChAT) catalyzes the synthesis of the neurotransmitter acetylcholine (ACh) from choline and acetyl-CoA in cholinergic neurons. ChAT serves as a specific marker for cholinergic neurons in both peripheral and central nervous systems. Evidence shows that ChAT exists in two forms inside cholinergic nerve terminals, a soluble hydrophilic form and the membrane-associated amphiphilic form.[1-2] […]
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The conjugation specialists at Advanced Targeting Systems are proud to announce a new addition to the catalog: MonoBiotin-ZAP Cat. #BT-ZAP Researchers now have more freedom in selecting conjugation strategies. Users have taken advantage of the ability to use biotinylated targeting agents with Streptavidin-ZAP (Cat. #IT-27) a chemical conjugate between saporin and streptavidin. Streptavidin-ZAP converts biotinylated
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A specific immunotoxin elucidates a causal role of striatal cholinergic system in behavioral flexibility by Sho Aoki and Jeffery R. Wickens Neurobiology Research Unit, Okinawa Institute of Science and Technology, 1919-1 Tancha, Onna, Kunigami, Okinawa 904-0495, Japan Behavioral flexibility is broadly defined as the ability to change behavioral strategy, according to a change of governing
Cover Article: Anti-ChAT-SAP elucidates a causal role in behavioral flexibility Read More »
Saporin conjugates specifically eliminate cells identified by an extracellular marker. Each week we release new products for beta testing, so check out the website frequently. There are two conjugate configurations for Beta products. The first is a direct conjugate of the targeting agent to saporin; the second is a bonded toxin between the targeting agent
Targeting Tools: Beta Products. Great Opportunity for Discovery at a Very Low Price. Read More »
While recombinant IB4-SAP (rIB4-SAP, Cat. #IT-10) has traditionally been used to eliminate cell populations that display alpha-D-galactose on the cell membrane, such as non-peptidergic c-fiber nociceptor neurons, it has also been found to be a very effective way to create stable transfected cell lines without the use of drug resistance genes. A recent publication by
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by Carl Noe, M.D., Anesthesiology & Pain Management, UT Southwestern Medical Center, Dallas, TX; and Eugene McDermott Center for Pain Management, Dallas, Texas Dr. Noe is the Principal Investigator in the ongoing trial of SP-SAP Intrathecal SP-SAP (Substance P attached to the ribosome-inactivating protein, saporin) has been studied in a Phase 1 clinical trial of
Custom Biotinylation, when the “one-step-kit” doesn’t quite meet your needs. Proteins come in all shapes and sizes and don’t always contain a ready-to-conjugate binding site. This and other variables can cause you to look toward one-step kits, often leaving you with un-purified and un-verified conjugate. Let the experts in the field help provide you with
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by Masahiro Sato1 (Ph. D.) and Satoshi Watanabe2 (Ph. D.) 1Section of Gene Expression Regulation, Frontier Science Research Center, Kagoshima University, Kagoshima 890-8544, Japan: 2Animal Genome Research Unit, Division of Animal Science, National Institute of Agrobiological Sciences, Ibaraki 305-8602, Japan Isolation of stable transfectants is one of the important steps for exploring biological functions of