antibodies

Zheng X, Zhai B, Koivunen P, Shin S, Lu G, Liu J, Geisen C, Chakraborty A, Moslehi J, Smalley D, Wei X, Chen X, Chen Z, Beres J, Zhang J, Tsao J, Brenner M, Zhang Y, Fan C, DePinho R, Paik J, Gygi S, Kaelin W, Zhang Q (2014) Prolyl hydroxylation by EglN2 destabilizes FOXO3a by blocking its interaction with the USP9x deubiquitinase. Genes Dev 28:1429-1444. doi: 10.1101/gad.242131.114 PMID: 24990963

Summary: Members of the FOXO family are thought to act as tumor suppressor genes. In this work the authors investigated the hydroxylation of FOXO3a by EglN2. This hydroxylation pushes FOXO3a toward a protosomal degradation pathway. Loss of FOXO3a in turn allows the accumulation of Cyclin D1, which has been found to be overexpressed in some breast cancers. Some of the data were generated using immunoblots with anti-transhydroxylated proline (Cat. #AB-T044).

Usage: Western blot

Related Products: Trans-4-Hydroxy-L-Proline Rabbit Polyclonal, Conjugated (Cat. #AB-T044)

Joly S, Guzik-Kornacka A, Schwab M, Pernet V (2014) New mouse retinal stroke model reveals direction-selective circuit damage linked to permanent optokinetic response loss. Invest Ophthalmol Vis Sci 55:4476-4489. doi: 10.1167/iovs.14-14521 PMID: 24970264

Summary: The authors used a mouse model of ‘retinal stroke’ to better delineate the optokinetic response deficits at the cellular level. Damage was found in the processes of starburst amacrine cells (SACs), and to a lesser extent, the dendrites. Anti-melanopsin (Cat. #AB-N38) at 1:2500 was used for immunohistochemistry.

Related Products: Melanopsin Rabbit Polyclonal (Cat. #AB-N38)

Bajo V, Leach N, Cordery P, Nodal F, King A (2014) The cholinergic basal forebrain in the ferret and its inputs to the auditory cortex. Eur J Neurosci 40:2922-2940. doi: 10.1111/ejn.12653 PMID: 24945075

Summary: The ferret has become a more common animal model in auditory neuroscience. Unlike rodent models, however, anatomical data describing the organization of the basal forebrain cholinergic system and its projections to the auditory cortex have not been well characterized. Using a variety of methods the authors mapped the architecture of the ferret basal forebrain. IHC was done with several antibodies including anti-ChAT (Cat. #AB-N34AP; 1:1000) and anti-NGFr (Cat. #AB-N07; 1:500). Animals also received 17 μg of ME20.4-SAP (Cat. #IT-15) in a total of 17 injections into the ectosylvian gyrus. The results indicate that acetylcholine is most likely involved in modulation of auditory processing.

Related Products: Choline Acetyltransferase Rabbit Polyclonal, affinity-purified (Cat. #AB-N34AP), NGFr (ME20.4, p75) Mouse Monoclonal (Cat. #AB-N07), ME20.4-SAP (Cat. #IT-15)

Akahoshi N, Kamata S, Kubota M, Hishiki T, Nagahata Y, Matsuura T, Yamazaki C, Yoshida Y, Yamada H, Ishizaki Y, Suematsu M, Kasahara T, Ishii I (2014) Neutral aminoaciduria in cystathionine β-synthase-deficient mice; an animal model of homocystinuria. Am J Physiol Renal Physiol 306:F1462-1476. doi: 10.1152/ajprenal.00623.2013 PMID: 24761004

Summary: The authors utilized a mouse model for homocystinuria in order to examine renal amino acid reabsorbtion. Some of the immunohistochemistry experiments used anti-Met (Cat. #AB-T036). It was found that loss of cystathionine β-synthase causes hyperexcretion of both glucogenic and ketogenic neutral amino acids, as well as histidine.

Zeltner N, Lafaille FG, Fattahi F, Studer L (2014) Feeder-free derivation of neural crest progenitor cells from human pluripotent stem cells. J Vis Exp 87:51609. doi: 10.3791/51609 PMID: 24893703

Related Products: NGFr (ME20.4, p75) Mouse Monoclonal (Cat. #AB-N07)

Forsyth PA, Krishna N, Lawn S, Valadez JG, Qu X, Fenstermacher DA, Fournier M, Potthast L, Chinnaiyan P, Gibney GT, Zeinieh M, Barker PA, Carter BD, Cooper MK, Kenchappa RS (2014) p75 neurotrophin receptor cleavage by α- and γ-secretases is required for neurotrophin-mediated proliferation of brain tumor-initiating cells. J Biol Chem 289(12):8067-8085. doi: 10.1074/jbc.M113.513762 PMID: 24519935

Kiris E, Wang T, Yanpallewar S, Dorsey S, Becker J, Bavari S, Palko M, Coppola V, Tessarollo L (2014) TrkA in vivo function is negatively regulated by ubiquitination. J Neurosci 34:4090-4098. doi: 10.1523/JNEUROSCI.4294-13.2014 PMID: 24623787

Summary: The high affinity nerve growth factor receptor, trkA, plays an intrinsic role in the regulation of various aspects of the mammalian nervous system. The post-translational attachment of ubiquitin to trkA plays a role in the final disposition and function of many proteins; in this work the authors investigate the result of trkA ubiquitination. By removing a 3 amino acid sequence from the receptor the ubiquitination of TrkA was reduced which resulted in an increase in TrkA protein levels and activity. In mice containing this mutation, the rise in TrkA activity was accompanied by enhanced thermal sensitivity and inflammatory pain. Anti-trkA (Cat. #AB-N03) was used at a concentration of 1:500 in immunohistochemistry.

Related Products: trkA Rabbit Polyclonal (Cat. #AB-N03)

Douglas AD, Williams AR, Knuepfer E, Illingworth JJ, Furze JM, Crosnier C, Choudhary P, Bustamante LY, Zakutansky SE, Awuah DK, Alanine DG, Theron M, Worth A, Shimkets R, Rayner JC, Holder AA, Wright GJ, Draper SJ (2014) Neutralization of Plasmodium falciparum merozoites by antibodies against PfRH5. J Immunol 192(1):245-258. doi: 10.4049/jimmunol.1302045 PMID: 24293631

Summary: Plasmodium falciparum is a protozoan parasite that can cause malaria in humans. The human malaria parasite reticulocyte –binding protein homolog 5 (PfRH5) is a potential target for neutralizing antibodies. PfRH5 binds basigin on erythrocytes, and through the use of anti-basigin (Cat. #AB-42), among other antibodies, the authors better characterized aspects of this binding that may be useful in preventing malaria.

Related Products: Antibody to Basigin (Cat. #AB-42)

Yin S, Noetzel MJ, Johnson KA, Zamorano R, Jalan-Sakrikar N, Gregory KJ, Conn PJ, Niswender CM (2014) Selective actions of novel allosteric modulators reveal functional heteromers of metabotropic glutamate receptors in the CNS. J Neurosci 34(1):79-94. doi: 10.1523/JNEUROSCI.1129-13.2014 PMID: 24381270

Summary: The eight mGlu receptor subtypes that have been discovered have been thought to function only as homodimers. Although recent studies have suggested that these receptors can form heterodimers in vitro, this has not been demonstrated in vivo. Using a variety of methods, including western blots, coimmunoprecipitation, thallium flux assays, transient transfections, and whole cell patch recordings the authors show that mGlu2 and mGlu4 form a hetero-complex in both rat and mouse tissues. An anti-mGluR2 (Cat. #AB-N32) was used in coimmunoprecipitation.

Related Products: Metabotropic Glutamate Receptor 2 (mGluR2) Mouse Monoclonal (Cat. #AB-N32)

Kinikoglu B, Kong Y, Liao EC (2014) Characterization of cultured multipotent zebrafish neural crest cells. Exp Biol Med (Maywood) 239(2):159-168. doi: 10.1177/1535370213513997 PMID: 24326414

Summary: This work details the isolation of neural crest cells (NCCs) from transgenic zebrafish embryos expressing GFP and flow cytometry; the authors analyzed lineage markers and differentiation of the NCCs. Anti-mu p75 (Cat. #AB-N01AP) was used in immunocytochemistry at a 1:20 dilution on fixed cells.

Related Products: NGFr (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)