Bajo V, Leach N, Cordery P, Nodal F, King A (2014) The cholinergic basal forebrain in the ferret and its inputs to the auditory cortex. Eur J Neurosci 40:2922-2940. doi: 10.1111/ejn.12653 PMID: 24945075
Summary: The ferret has become a more common animal model in auditory neuroscience. Unlike rodent models, however, anatomical data describing the organization of the basal forebrain cholinergic system and its projections to the auditory cortex have not been well characterized. Using a variety of methods the authors mapped the architecture of the ferret basal forebrain. IHC was done with several antibodies including anti-ChAT (Cat. #AB-N34AP; 1:1000) and anti-NGFr (Cat. #AB-N07; 1:500). Animals also received 17 μg of ME20.4-SAP (Cat. #IT-15) in a total of 17 injections into the ectosylvian gyrus. The results indicate that acetylcholine is most likely involved in modulation of auditory processing.