antibodies

Yao P, Ding Y, Han Z, Mu Y, Hong T, Zhu Y, Li H (2017) Suppression of asparaginyl endopeptidase attenuates breast cancer-induced bone pain through inhibition of neurotrophin receptors. Mol Pain 13:744806917708127. doi: 10.1177/1744806917708127 PMID: 28554249

Objective: To determine the functions and targeted suppression of asparaginyl endopeptidase (AEP) in a mouse model of breast cancer-induced bone pain.

Summary: Targeted suppression of AEP with specific small compounds significantly reduced bone pain while purified recombinant AEP proteins increased bone pain. AEP aggravates the development of breast cancer bone metastasis and bone pain by increasing the expression of neurotrophin receptors. AEP might be an effective target for treatment of breast cancer-induced bone pain.

Usage: Western blot

Related Products: NGFR (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Wu W, Wu XL, Ji YQ, Gao Z (2017) Differentiation of nestin‑negative human hair follicle outer root sheath cells into neurons in vitro. Mol Med Rep 16(1):95-100. doi: 10.3892/mmr.2017.6585 PMID: 28534946

Objective: To isolate nestin-negative outer root sheath cells (ORSCs) from adult healthy volunteers and investigate the neuronal differentiation of these cells.

Summary: The present study did not verify the value of neural differentiation into sphere-forming cells for therapeutic application, however, the differentiation of nestin-negative human hair follicle ORSCs into neurons in vitro may serve as a theoretical underpinning for neurodegenerative diseases and regenerative medicine.

Usage: IF (1:250)

Related Products: NGFR (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Dorard C, Estrada C, Barbotin C, Larcher M, Garancher A, Leloup J, Beermann F, Baccarini M, Pouponnot C, Larue L, Eychène A, Druillennec S (2017) RAF proteins exert both specific and compensatory functions during tumour progression of NRAS-driven melanoma. Nat Commun 8:15262. doi: 10.1038/ncomms15262 PMID: 28497782

Objective: To investigate the contribution of Raf genes in tumor progression, from the onset of benign tumors to malignant tumor maintenance, in NRAS-induced mouse melanoma models

Summary: BRAF expression is required for ERK activation and nevi development, demonstrating a critical role in the early stages of NRAS-driven melanoma. After melanoma formation, single BRAF or CRAF ablation is not sufficient to block tumor growth, showing redundant functions for RAF kinases. Proliferation of resistant cells emerging in the absence of BRAF and CRAF remains dependent on ARAF-mediated ERK activation.

Usage: Cell sorting by Flow Cytometry (1:500)

Related Products: NGFR (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Guo JR, Wang H, Jin XJ, Jia DL, Zhou X, Tao Q (2017) Effect and mechanism of inhibition of PI3K/Akt/mTOR signal pathway on chronic neuropathic pain and spinal microglia in a rat model of chronic constriction injury. Oncotarget 8(32):52923-52934. doi: 10.18632/oncotarget.17629 PMID: 28881783

Objective: To explore the effects of inhibition of PI3K/Akt/mTOR signal pathway on chronic neuropathic pain (CNP) and spinal microglia in a rat model of chronic constriction injury (CCI).

Summary: Inhibition of PI3K/Akt/mTOR signal pathway may alleviate CNP and reduce microglia and GFAP and NGF expressions in marrow in a rat model of CCI.

Usage: immunofluorescence assay (1:500)

Related Products: NGFR (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Kulkarni S, Micci MA, Leser J, Shin C, Tang SC, Fu YY, Liu L, Li Q, Saha M, Li C, Enikolopov G, Becker L, Rakhilin N, Anderson M, Shen X, Dong X, Butte MJ, Song H, Southard-Smith EM, Kapur RP, Bogunovic M, Pasricha PJ (2017) Adult enteric nervous system in health is maintained by a dynamic balance between neuronal apoptosis and neurogenesis. Proc Natl Acad Sci U S A 114(18):E3709-E3718. doi: 10.1073/pnas.1619406114 PMID: 28420791

Objective: To identify adult enteric neural precursor cell (ENPC) that would enable a new understanding of the pathogenesis of enteric neuromuscular diseases as well as the development of novel regenerative therapies.

Summary: The results show significant turnover and neurogenesis of adult enteric neurons and provide a paradigm for understanding the enteric nervous system in health and disease.

Usage: IHC (1:400)

Related Products: NGFR (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Chen PC, Hsieh MH, Kuo WS, Kao HF, Hsu CL, Wang JY (2017) Water-soluble chitosan inhibits nerve growth factor and attenuates allergic inflammation in mite allergen-induced allergic rhinitis. J Allergy Clin Immunol 140(4):1146-1149.e8. doi: 10.1016/j.jaci.2017.03.022 PMID: 28412394

Objective: To evaluate the effect of extensive water-soluble chitosan (EWSC) in Allergic rhinitis (AR).

Summary: The results suggest that NGF- and TH2-related cytokines created a positive feedback loop amplifying allergic inflammation in the upper-airway epithelial cells, and EWSC attenuated allergic inflammation and epithelial cell damage, by inhibiting NGF biosynthesis during allergic TH2 immune responses.

Usage: IHC

Related Products: NGFR (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Cesarini V, Guida E, Todaro F, Di Agostino S, Tassinari V, Nicolis S, Favaro R, Caporali S, Lacal PM, Botti E, Costanzo A, Rossi P, Jannini EA, Dolci S (2017) Sox2 is not required for melanomagenesis, melanoma growth and melanoma metastasis in vivo. Oncogene 36(31):4508-4515. doi: 10.1038/onc.2017.53 PMID: 28368402

Objective: To investigate whether Sox2 is required during the process of melanomagenesis, melanoma growth and metastasis and in the acquisition of resistance to BRAF inhibitors (BRAFi) treatments.

Summary: The findings exclude an oncogenic function for Sox2 during melanoma development and do not support a role for this transcription factor in the acquisition of resistance to BRAFi treatments.

Usage: Immunofluorescence on melanoma cell lines

Related Products: NGFR (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Huang L, Yuan T, Tan M, Xi Y, Hu Y, Tao Q, Zhao Z, Zheng J, Han Y, Xu F, Luo M, Sollars P, Pu M, Pickard G, So K, Ren C (2017) A retinoraphe projection regulates serotonergic activity and looming-evoked defensive behaviour. Nat Commun 8:14908. doi: 10.1038/ncomms14908 PMID: 28361990

Objective: To investigate how the dorsal raphe nucleus (DRN) and superior colliculus work in concert to extract and translate visual threats into defensive behavioural responses.

Summary: A dedicated population of RGCs signals rapidly approaching visual threats and their input to the DRN controls a serotonergic self-gating mechanism that regulates innate defensive responses.

Usage: Mice received bilateral intraocular injection (2 μg per eye) made between Streptavidin-Saporin and a biotinylated CTB antibody, or Anti-Melanopsin-SAP (2 μg per eye). For detection of melanopsin, retinas were incubated for 3 days at 4 °C with anti-melanopsin (1:600).

Related Products: Streptavidin-ZAP (Cat. #IT-27), Melanopsin-SAP (Cat. #IT-44), Melanopsin Rabbit Polyclonal (Cat. #AB-N38)

Kobayashi K, Maezawa T, Tanaka H, Onuki H, Horiguchi Y, Hirota H, Ishida T, Horiike K, Agata Y, Aoki M, Hoshi M, Matsumoto M (2017) The identification of ᴅ-tryptophan as a bioactive substance for postembryonic ovarian development in the planarian Dugesia ryukyuensis. Sci Rep 7:45175. doi: 10.1038/srep45175 PMID: 28338057

Usage: IHC 1:50

Ekman M, Zhu B, Swärd K, Uvelius B (2017) Neurite outgrowth in cultured mouse pelvic ganglia – Effects of neurotrophins and bladder tissue. Auton Neurosci 205:41-49. doi: 10.1016/j.autneu.2017.03.004 PMID: 28347639

Objective: To characterize the effects of the neurotrophins nerve growth factor (NGF), brain derived neurotrophic factor (BDNF) and neurotrophin 3 (NT-3) on the sprouting rate of sympathetic and parasympathetic neurites from the female mouse ganglion.

Summary: All three factors (BDNF, NT-3 and NGF) stimulated neurite outgrowth of both sympathetic and parasympathetic neurites although BDNF and NT-3 had a higher stimulatory effect on parasympathetic ganglion cells. Active forms of BDNF and NT-3 were detected in urinary bladder tissue, whereas tissue from the diaphragm expressed NGF.

Usage: in vitro culture (2 ul/ml)

Related Products: NGFR (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)