antibodies

Lindborg JA, Mack M, Zigmond RE (2017) Neutrophils are critical for myelin removal in a peripheral nerve injury model of Wallerian degeneration. J Neurosci 37(43):10258-10277. doi: 10.1523/JNEUROSCI.2085-17.2017 PMID: 28912156

Objective: To characterize Wallerian degeneration (WD) in male Ccr2−/−mice and identify a compensatory mechanism of WD that is facilitated primarily by neutrophils.

Summary: The data have shown that neutrophils play an impressive role in myelin removal during WD. Neutrophil-specific depletion severely abates nerve debris clearance not only in Ccr2−/− mice, but in WT mice as well. The findings show a novel role for neutrophils that better elucidates the process of WD in the peripheral nervous system.

Usage: immunohistochemistry (1:400)

Related Products: NGFR (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Cai S, Tsui YP, Tam KW, Shea GK, Chang RS, Ao Q, Shum DK, Chan YS (2017) Directed differentiation of human bone marrow stromal cells to fate-committed schwann cells. Stem Cell Reports 9(4):1097-1108. doi: 10.1016/j.stemcr.2017.08.004 PMID: 28890164

Objective: To create a protocol for in vitro derivation of fate-committed Schwann cells (SCs) from human bone marrow stromal cells (BMSCs).

Summary: This study demonstrates that the human bone marrow harbors neuro-ectodermal progenitors that can be enriched, expanded, and directed to differentiate into functionally mature, fate-committed SCs.

Usage: IF (1:500) and WB (1:500)

Related Products: NGFR (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Li G, Liu J, Wang Y, Yang K, Zhao M, Xiao Y, Wen X, Liu L (2017) LNGFR targets the Wnt/β-catenin pathway and promotes the osteogenic differentiation in rat ectomesenchymal stem cells. Sci Rep 7(1):11021. doi: 10.1038/s41598-017-11555-9 PMID: 28887537

Objective: To demonstrate that nuclear β-catenin was upregulated during ectomesenchymal stem cells (EMSC) osteogenic differentiation.

Summary: The data reveal that the low affinity nerve growth factor receptor (LNGFR) targets the Wnt/β-catenin pathway and positively regulates EMSC osteogenic differentiation, suggesting that Wnt/β-catenin pathway may be involved in the development of teeth and that the targeting Wnt/β-catenin pathway may have great potential for applications in dental tissue engineering regeneration.

Usage: Western blot

Related Products: NGFR (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Yu X, Liu Z, Hou R, Nie Y, Chen R (2017) Nerve growth factor and its receptors on onset and diagnosis of ovarian cancer. Oncol Lett 14(3):2864-2868. doi: 10.3892/ol.2017.6527 PMID: 28928825

Objective: To investigate the effect of nerve growth factor (NGF) and its receptors on the onset and diagnosis of ovarian cancer.

Summary: NGF and its receptor can contribute to the occurrence of ovarian cancer, and the onset condition of ovarian cancer can be diagnosed through the detection of high or low expression of NGF and its receptors.

Usage: ELISA, WB, IHC (1:500)

Related Products: NGFR (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Wang G, Ma Z, Cao L, Yan G, Wang Y, Jin Y, Shen H, Zhang Y, Xu X, Chen X, Shen Z (2017) A novel method for obtaining highly enriched Schwann cell populations from mature monkey nerves based on in vitro pre‑degeneration. Mol Med Rep 16(5):6600-6607. doi: 10.3892/mmr.2017.7427 PMID: 28901508

Objective: To identify a novel technique that can efficiently isolate and purify Schwann cells (SCs) from mature monkey nerves based on in vitro pre-degeneration.

Summary: Based on their observations, the migration rate of SCs out of the nerve explants appeared to reach its maximum after 14 days. However, the level of fibroblast proliferation was also significantly increased, which reduced the purity of the SCs. Therefore, the shorter 7-day pre-degeneration period may be optimal to maximize SC purity. These Schwann cells may be used to provide support for regenerating peripheral or central nerves.

Usage: Immunofluorescence (1:500) and flow cytometry

Related Products: NGFR (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Falade TE, Olude MA, Mustapha OA, Mbajiorgu EF, Ihunwo AO, Olopade JO, Oke BO (2017) Connective tissue, glial and neuronal expressions in testis of the African giant rat (Cricetomys gambianus). J Morphological Sciences 34(03):186-193. doi: 10.4322/jms.109117

Objective: To investigate the expression of connective tissue (Collagens I and III), glia and neuronal markers in the testis of the African giant rat.

Summary: The cyto-architecture of the testis was typical in the African giant rat. The connective tissue expressed in the juvenile and adult group, signaling of glial-like cells were seen in the perivascular region across the experimental groups. Immuno-localization of neuronal cells was seen in the interstitial spaces across all the groups, but with more expressions in the juvenile.

Usage: IHC (1:700)

Related Products: NGFR (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Bray ER, Noga M, Thakor K, Wang Y, Lemmon VP, Park KK, Tsoulfas P (2017) 3D visualization of individual regenerating retinal ganglion cell axons reveals surprisingly complex growth paths. eNeuro 4:ENEURO.0093-0017.2017. doi: 10.1523/ENEURO.0093-17.2017 PMID: 28856242

Summary: “Our study demonstrates extensive and circuitous RGC axon elongation both in pre- and post-lesion regions, highlighting the need to better understand the factors that inhibit direct axon growth in the optic nerve.”

Usage: Immunohistochemistry: Rabbit-anti-melanopsin (OPN4; UF006) 1:2500 (Cat. #AB-N38)

Related Products: Melanopsin Rabbit Polyclonal (Cat. #AB-N38)

Brick, R (2017) Induced pluripotent stem cell-derived mesenchymal progenitor cells: Promotion of neuritogenesis and axon elongation through neurotrophin and cytokine production. University of Pittsburgh Thesis.

Objective: To test if mesenchymal progenitor cells (MiMPCs), originally derived from mesenchymal stem cells (MSCs), have the same ability to support neuronal regeneration as the parent MSC.

Summary: Results show that MiMPCs can secrete neurotrophic factors after neuroinductive treatment and can produce factors to improve neurite outgrowth in a chick embryonic dorsal root ganglion (DRG) model. These findings suggest that neurotrophically induced-MiMPCs (NI-MiMPCs) may be considered a suitable substitute cell type to support nerve growth.

Usage: immunofluorescence

Related Products: NGFR (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Lai FP, Lau ST, Wong JK, Gui H, Wang RX, Zhou T, Lai WH, Tse HF, Tam PK, Garcia-Barcelo MM, Ngan ES (2017) Correction of Hirschsprung-associated mutations in human induced pluripotent stem cells via clustered regularly interspaced short palindromic repeats/cas9, restores neural crest cell function. Gastroenterology 153(1):139-153.e8. doi: 10.1053/j.gastro.2017.03.014 PMID: 28342760

Objective: To identify mutations associated with short-segment Hirschsprung disease (S-HSCR), and use the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 gene editing system to determine how mutations affect enteric neural crest cells (ENCC) function.

Summary: Mutations in vinculin gene (VCL) associated with S-HSCR were identified. Correction of this mutation in iPSC using CRISPR/Cas9 editing, as well as the RET G731del mutation that causes Hirschsprung disease with total colonic aganglionosis, restored ENCC function. The study demonstrates how human iPSCs can be used to identify disease-associated mutations and determine how they affect cell functions and contribute to pathogenesis.

Usage: Immunofluorescence (1:500)

Related Products: NGFR (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Casciello F, Al-Ejeh F, Kelly G, Brennan D, Ngiow S, Young A, Stoll T, Windloch K, Hill M, Smyth M, Gannon F, Lee J (2017) G9a drives hypoxia-mediated gene repression for breast cancer cell survival and tumorigenesis. Proc Natl Acad Sci U S A 114:7077-7082. doi: 10.1073/pnas.1618706114 PMID: 28630300

Usage: Western blot

Related Products: Trans-4-Hydroxy-L-Proline Rabbit Polyclonal, Conjugated (Cat. #AB-T044)