antibodies

Anacker A, Esser KH, Lenarz T, Paasche G (2022) Purification of fibroblasts from the spiral ganglion. Front Neurol 13:877342. doi: 10.3389/fneur.2022.877342 PMID: 35493807

Objective: To provide a purified preparation of cochlear fibroblasts, which can be used in vitro for further investigations.

Summary: Subcultivation of the primary spiral ganglion cells culture removed the neurons from the culture and increased the fibroblast to glial cell ratio in the preparations. Direct immunolabeling for the Thy1-glycoprotein and the p75NGFR-enabled fluorescence-based cell sorting. This resulted in a cell culture of cochlear fibroblasts with a purity of more than 99%.

Usage: Cell Sorting (1:400)

Related Products: NGFr (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Lee DS, Kim JE (2022) P2X7 receptor augments LPS-induced nitrosative stress by regulating Nrf2 and GSH levels in the mouse hippocampus. Antioxidants (Basel) 11(4):778. doi: 10.3390/antiox11040778 PMID: 35453462

Objective: To elucidate whether P2X7R affects nuclear factor-erythroid 2-related factor 2 (Nrf2) activity/expression and glutathione (GSH) synthesis under nitrosative stress in response to lipopolysaccharide (LPS)-induced neuroinflammation.

Summary: The findings indicate that P2X7R may augment LPS-induced neuroinflammation by leading to Nrf2 degradation, aberrant glutamate-glutamine cycle, and impaired cystine/cysteine uptake, which would inhibit GSH biosynthesis.

Usage: Immunohistochemistry (1:1000)

Related Products: NO-L-Cysteine Mouse Monoclonal, Conjugated (Cat. #AB-T125)

Sabbah S, Papendorp C, Behrendt I, Rasras H, Cann J, Leyrer ML, Koplas E, Beltoja M, Etebari C, Gunesch AN, Carrete L, Kim MT, Manoff G, Bhatia-Lin A, Zhao T, Dowling H, Briggman KL, Berson DM (2022) Intrinsically photosensitive retinal ganglion cells evade temporal filtering to encode environmental light intensity. bioRxiv 2022.04.09.487733. doi: 10.1101/2022.04.09.487733

Related Products: Melanopsin Rabbit Polyclonal (Cat. #AB-N38)

Liu G, Chen Y, Wang Y, Deng X, Xiao Q, Zhang L, Xu H, Han X, Lei A, He J, Li X, Cao Y, Zhou P, He C, Wu P, Jiang W, Tan M, Chen C, Yang Q, Lu L, Deng K, Yao Z, Zhou J (2022) Angiotensin II enhances group 2 innate lymphoid cell responses via AT1a during airway inflammation. J Exp Med 219(3):e20211001. doi: 10.1084/jem.20211001 PMID: 35044462

Objective: To identify angiotensin (Ang) II as a positive regulator of Group 2 innate lymphoid cells (ILC2s).

Summary: Ang II plays a critical role in regulating ILC2 responses and airways inflammation.

Usage: Administration of Ang II (30ug) i.n. or i.p. daily for 5 days.

Related Products: Angiotensin II receptor (AT-1AR) Rabbit Polyclonal, affinity-purified (Cat. #AB-N25AP)

Aparicio Prieto MV, Rodríguez Gallego MV, Valdivia Palacín A, Franco Iriarte Y, Hervás Barbara G, Echevarría Orella E, Casis Saenz L (2022) Local renin angiotensin system and sperm DNA fragmentation. Asian J Androl 24(2):139-146. doi: 10.4103/aja202150 PMID: 34494558

Objective: The renin angiotensin system (RAS) appears to influence male fertility at multiple levels. The relationship between the RAS and DNA integrity was analyzed.

Summary: Fragmented DNA spermatozoa have a lower capacity to respond to bioactive RAS peptides.

Usage: Immunocytochemistry

Related Products: Angiotensin II receptor (AT-2R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N28AP)

Serapiglia V, Stephens CA, Joshi R, Aydin E, Oria M, Marotta M, Peiro JL, Varisco BM (2022) Fetal tracheal occlusion increases lung basal cells via increased yap signaling. Front Pediatr 9:780166. doi: 10.3389/fped.2021.780166 PMID: 35280447

Objective: To test if lung epithelial cell populations alter after tracheal occlusion (TO) with transcriptomic signatures implicating basal cells.

Summary: TO increases lung epithelial cell nuclear Yap, leading to basal cell expansion.

Usage: Immunohistochemistry (1:1000)

Related Products: NGFr (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Cleverdon REG, Whitley KC, Marko DM, Hamstra SI, Braun JL, Roy BD, MacPherson REK, Fajardo VA (2021) Sarcoplasmic reticulum Ca2+ handling in young DBA/2J vs C57BL/10 mdx models of Duchenne muscular dystrophy. bioRxiv 2021.10.25.465805. doi: 10.1101/2021.10.25.465805

Objective: To investigate whether sarco(endo)plasmic reticulum Ca2+-ATPase (SERCA) function would differ in muscles from young D2 and C57 mdx mice.

Summary: The study demonstrates that SERCA function is drastically impaired in young D2 mdx mice.

Usage: Western blot

Related Products: NO-L-Cysteine Mouse Monoclonal, Conjugated (Cat. #AB-T125)

Lin HJ, Mahendran R, Huang HY, Chiu PL, Chang YM, Day CH, Chen RJ, Padma VV, Liang-Yo Y, Kuo WW, Huang CY (2021) Aqueous extract of Solanum nigrum attenuates Angiotensin-II induced cardiac hypertrophy and improves cardiac function by repressing protein kinase C-ζ to restore HSF2 deSUMOlyation and Mel-18-IGF-IIR signaling suppression. J Ethnopharmacol 8:114728. doi: 10.1016/j.jep.2021.114728 PMID: 34634367

Objective: To investigate the ability of Solanum nigrum to attenuate Angiotensin II – induced cardiac hypertrophy and improve cardiac function through the suppression of protein kinase PKC-ζ and Mel-18-IGF-IIR signaling leading to the restoration of HSF2 desumolyation.

Summary: The data reveals the cardioprotective effect of S. nigrum inhibiting PKC-ζ with alleviated IGF IIR level in the heart that profoundly remits cardiac hypertrophy for hypertension-induced heart failure.

Usage: Western Blot

Related Products: Angiotensin II receptor (AT-2R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N28AP)

Amano M, Amiya N, Fukushima K, Hagio H, Yamamoto N, Sakakura Y (2022) Effects of crowding stress on the hypothalamo-pituitary-interrenal axis of the self-fertilizing fish, Kryptolebias marmoratus. Comp Biochem Physiol A Mol Integr Physiol 264:111110. doi: 10.1016/j.cbpa.2021.111110 PMID: 34737084

Objective: To test whether crowding stress affects the hypothalamo-pituitary-interrenal (HPI) axis of the self-fertilizing fish, Kryptolebias marmoratus.

Summary: The number of CRH-ir cell bodies and cortisol levels were significantly higher in paired fish. The results indicate that crowding stress affects the HPI axis in K. marmoratus which thrive in small burrows with limited water volume.

Usage: Immunohistochemistry (1:2000)

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Napoli M, Wu SJ, Gore BL, Abbas HA, Lee K, Checker R, Dhar S, Rajapakshe K, Tan AC, Lee MG, Coarfa C, Flores ER (2022) ΔNp63 regulates a common landscape of enhancer associated genes in non-small cell lung cancer. Nat Commun 13(1):614. doi: 10.1038/s41467-022-28202-1 PMID: 35105868

Objective: To investigate the underlying mechanistic role regulated by ΔNp63 in lung cancer development.

Summary: Use of a ΔNp63-specific conditional knockout mouse model and xenograft models of lung adenocarcinoma (LUAD) and squamous cell carcinoma (LUSC). Results show that ΔNp63 promotes non-small cell lung cancer by maintaining the lung stem cells necessary for lung cancer cell initiation and progression in quiescence. ΔNp63 regulates enhancers of cell identity genes.

Usage: Immunofluorescence and IHC (1:100)

Related Products: NGFr (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)