Gastfriend BD, Palecek SP, Shusta EV (2025) Differentiation of brain mural cells from human pluripotent stem cell-derived neural crest by activation of notch3 signaling. (eds. Barichello, T.). Blood-Brain Barrier 221:11-35. Humana, New York, NY. doi: 10.1007/978-1-0716-4474-4_2
Objective: To describe a serum-free method to differentiate brain mural cells from human pluripotent stem cells (hPSCs), using lentiviral overexpression of the Notch3 intracellular domain in a neural crest intermediate.
Summary: Protocols were given on how to perform Flow cytometry, Neural crest differentiation and Lentivirus production .
Usage: Anti-NGFR (ME20.4, p75) Mouse Monoclonal, Flow Cytometry Analysis of neural crest cells (1:500)
Related Products: NGFR (ME20.4, p75) Mouse Monoclonal (Cat. #AB-N07)