antibodies

Dyer B, Yu SO, Lane Brown R, Lang RA, D’Souza SP (2024) A new Opn4cre recombinase mouse line to target intrinsically photosensitive retinal ganglion cells (ipRGCs). bioRxiv 2024.04.16.589750. doi: 10.1101/2024.04.16.589750 PMID: 38659888

Objective: To generate a new Opn4cre knock-in allele [Opn4cre(DSO)], in which cre is placed immediately downstream of the Opn4 start codon.

Summary: The Opn4cre(DSO) mouse line improves the specificity of ipRGC labeling, enabling the targeted study of these cells in relation to light-regulated behaviors and physiology.

Usage: Histology and immunofluorescence.

Related Products: Melanopsin Rabbit Polyclonal, affinity-purified (Cat. #AB-N39)

Zuppone S, Zarovni N, Noguchi K, Loria F, Morasso C, Lõhmus A, Nakase I, Vago R (2024) Novel loading protocol combines highly efficient encapsulation of exogenous therapeutic toxin with preservation of extracellular vesicles properties, uptake and cargo activity. Discov Nano 19(1):76. doi: 10.1186/s11671-024-04022-8 PMID: 38691254

Objective: Extracellular vesicles (EVs) have been investigated as carriers of biological therapeutics such as proteins and RNA as well as small-molecule drugs. The objective was to test a strategy of EV loading based on temporary pH alteration through incubation of EVs with alkaline sodium carbonate, which results in conspicuous exogenous molecule incorporation.

Summary: The encapsulated saporin resulted protected from degradation and was efficiently conveyed to receiving cancer cells and triggered cell death. EV-delivered saporin was more cytotoxic compared to the free toxin. This approach allows both the structural preservation of vesicle properties and the transfer of protected cargo in the context of drug delivery.

Usage: Authors used fluorescently labeled saporin, SAP-FITC, and a nano-sized EV-to-cargo ratio of 1:1.5 (w:w).

Related Products: Saporin Goat Polyclonal, affinity-purified FITC-labeled (Cat. #AB-15AP-FL)

Nord C, Jones I, Garcia-Maestre M, Hägglund AC, Carlsson L (2024) Reduced mTORC1-signaling in progenitor cells leads to retinal lamination deficits. Dev Dyn 253(10):922-939. doi: 10.1002/dvdy.707 PMID: 38546215

Objective: To demonstrate that mTORC1 mediates critical roles during neuronal lamination using the mouse retina as a model system.

Summary: This study establishes a critical role for mTORC1-signaling during retinal lamination and demonstrates that this pathway regulates diverse developmental mechanisms involved in driving the stratified arrangement of neurons during CNS development.

Usage: Immunohistochemistry (AB-N38) (1:1000).

Related Products: Melanopsin Rabbit Polyclonal (Cat. #AB-N38)

Huang M, Fang W, Farrel A, Li L, Chronopoulos A, Nasholm N, Cheng B, Zheng T, Yoda H, Barata MJ, Porras T, Miller ML, Zhen Q, Ghiglieri L, McHenry L, Wang L, Asgharzadeh S, Park J, Gustafson WC, Matthay KK, Maris JM, Weiss WA (2024) ALK upregulates POSTN and WNT signaling to drive neuroblastoma. Cell Rep 43(3):113927. doi: 10.1016/j.celrep.2024.113927 PMID: 38451815

Objective: To determine how anaplastic lymphoma kinase (ALK) contributes to tumor formation.

Summary: ALK partially drives neuroblastoma through a feedforward loop between POSTN and WNT signaling.

Usage: AB-N07 Anti-NGFR Immunofluorescence (1:250).

Related Products: NGFR (ME20.4, p75) Mouse Monoclonal (Cat. #AB-N07)

Qian C, Xin Y, Qi C, Wang H, Dong BC, Zack DJ, Blackshaw S, Hattar S, Zhou FQ, Qian J (2024) Intercellular communication atlas reveals Oprm1 as a neuroprotective factor for retinal ganglion cells. Nat Commun 15(1):2206. doi: 10.1038/s41467-024-46428-z PMID: 38467611

Objective: To explore how intercellular communication contributes to retinal ganglion cell (RGC) survival following optic nerve crush based on single-cell RNA-seq analysis.

Summary: The overall scores of the responsive interactions among the retinal cell types correlated with the distress interactions sent from RGCs.

Usage: Immunohistochemistry (1:500; AB-N38).

Related Products: Melanopsin Rabbit Polyclonal (Cat. #AB-N38)

Hernández-Barranco A, Santos V, Mazariegos MS, Caleiras E, Nogués L, Mourcin F, Léonard S, Oblet C, Genebrier S, Rossille D, Benguría A, Sanz A, Vázquez E, Dopazo A, Efeyan A, Ortega-Molina A, Cogne M, Tarte K, Peinado H (2024) NGFR regulates stromal cell activation in germinal centers. Cell Rep 43(2):113705. doi: 10.1016/j.celrep.2024.113705 PMID: 38307025

Objective: To study the effect of NGFR loss on lymph node organization and function, demonstrating that NGFR depletion leads to spontaneous germinal center (GC) formation and an expansion of the GC B cell compartment.

Summary: Results show that NGFR is involved in maintaining GC structure and function, participating in GC activation, antibody production, and immune tolerance.

Usage: Anti-NGFR Alexa488-labeled used in Flow Cytometry (1:200) (Cat# AB-N01AP-FLA).

Related Products: NGFR (mu p75) Rabbit Polyclonal, affinity-purified Alexa488-labeled (Cat. #AB-N01AP-FLA)

Rodríguez-Ramírez KT, Norte-Muñoz M, Lucas-Ruiz F, Gallego-Ortega A, Calzaferri F, García-Bernal D, Martínez CM, Galindo-Romero C, de Los Ríos C, Vidal-Sanz M, Agudo-Barriuso M (2024) Retinal response to systemic inflammation differs between sexes and neurons. Front Immunol 15:1340013. doi: 10.3389/fimmu.2024.1340013 PMID: 38384465

Objective: To examine how systemic inflammation, induced by intraperitoneal administration of lipopolysaccharide (LPS), affects the retina of male and female mice. The study also evaluates whether blocking the NLRP3 inflammasome and the extrinsic apoptosis pathway provides retinal protection.

Summary: Systemic LPS exposure leads to neuronal and sex-specific adverse effects in the mouse retina. These effects are mitigated by inhibiting the NLRP3 inflammasome and the extrinsic apoptosis pathway, highlighting their protective roles.

Usage: Immunodetection (1:750; AB-N39)

Related Products: Melanopsin Rabbit Polyclonal, affinity-purified (Cat. #AB-N39)

Maloney R, Quattrochi L, Yoon J, Souza R, Berson D (2024) Efficacy and specificity of melanopsin reporters for retinal ganglion cells. J Comp Neurol 532(2):e25591. doi: 10.1002/cne.25591 PMID: 38375612

Objective: To evaluate the precision and comprehensiveness of various labeling methods for intrinsically photosensitive retinal ganglion cells (ipRGCs).

Summary: The authors provide a comparative analysis of the strengths and limitations of each approach and highlight the need for tailored methods based on specific research applications.

Usage: Immunohistochemistry (1:10,000) (AB-N38).

Related Products: Melanopsin Rabbit Polyclonal (Cat. #AB-N38)

Matcham AC, Toma K, Tsai NY, Sze CJ, Lin PY, Stewart IF, Duan X (2024) Cadherin-13 Maintains Retinotectal Synapses via Transneuronal Interactions. J Neurosci 44(5):e1310232023. doi: 10.1523/JNEUROSCI.1310-23.2023 PMID: 38123991

Objective: To explore the role of Type II cadherins, particularly Cdh13, in the formation of specific retinotectal synapses.

Summary: Cdh13 is highly expressed in wide-field neurons of the superficial superior colliculus (sSC), and its removal from presynaptic retinal ganglion cells (RGCs) leads to a significant reduction in dendritic spines on postsynaptic wide-field neurons. Unlike αRGCs and On–Off Direction-Selective Ganglion Cells (ooDSGCs), Cdh13-expressing RGCs utilize distinct mechanisms to establish precise retinotectal connections.

Usage: Immunohistochemistry (1:500).

Related Products: Melanopsin Rabbit Polyclonal (Cat. #AB-N38), Melanopsin Rabbit Polyclonal, affinity-purified (Cat. #AB-N39)

Matynia A, Recio BS, Myers Z, Parikh S, Goit RK, Brecha NC, Pérez de Sevilla Müller L (2024) Preservation of intrinsically photosensitive retinal ganglion cells (ipRGCs) in late adult mice: Implications as a potential biomarker for early onset ocular degenerative diseases. Invest Ophthalmol Vis Sci 65(1):28. doi: 10.1167/iovs.65.1.28 PMID: 38224335

Objective: To assess the preservation of intrinsically photosensitive retinal ganglion cells (ipRGCs) in late adult mice and evaluate their potential as biomarkers for early onset ocular degenerative diseases.

Summary: This study investigates the stability of ipRGC morphology and function in mice aged 6 to 12 months, revealing that ipRGCs maintain their dendritic complexity and associated behavioral functions, such as pupillary light reflex and contrast sensitivity, during this period. These findings suggest that the consistent preservation of ipRGCs in late adulthood may serve as a valuable biomarker for early detection of ocular degenerative diseases, including Alzheimer’s, Parkinson’s, and diabetes.

Usage: Whole mount retinas were incubated with Anti-Melanopsin (AB-N39) at 1:1000 for 7 days at 4°C.

Related Products: Melanopsin Rabbit Polyclonal, affinity-purified (Cat. #AB-N39)