References

Burd E, Attari M, Smith C, Bunyak F, Lever, T, Nichols N (2025) Genioglossus sarcomere and mitochondria morphology in a rodent model of hypoglossal motor neuron death. Am Physiol Soc 40(S1) doi: 10.1152/physiol.2025.40.S1.0879

Objective: To develop a novel rodent model using intralingual injections of cholera toxin B conjugated to saporin (CTB-SAP) to induce targeted loss of XII LMNs and XII motor output

Summary: Authors hypothesized that XII LMN loss leads to degenerative changes in genioglossus sarcomere and mitochondria morphology in CTB-SAP rats. Ultrastructural changes in genioglossus sarcomere and mitochondria morphology were studied. The preliminary data suggests a dysregulation of sarcomere ultrastructure, particularly Z-lines, and a significant increase in mitochondrial perimeter and maximum Feret’s diameter in CTB-SAP rats vs. controls, consistent with degenerative changes and a potential in flammatory response in genioglossus muscle cells following the loss of XII LMNs.

Usage: Intralingual injection of adult male rats with CTB-SAP (IT-14)

Related Products: CTB-SAP (Cat. #IT-14)

Lewis R, Smith C, Burd E, Nichols N (2025) Phrenic motor nucleus astrocytic density and morphology in a rodent model of respiratory motor neuron death. Am Physiol Soc 40(S1) doi: 10.1152/physiol.2025.40.S1.1115

Objective: Authors hypothesized that astrocytic density would be increased and astrocytic morphology would be altered in the phrenic motor nucleus over the course of motor neuron death in CTB-SAP rats vs. controls.

Summary: The number of astrocytes was significantly increased in CTB-SAP-treated rats. Furthermore, an astrocytic morphological analysis revealed that CTB-SAP rats have significantly increased branch number, number of endpoints, filament volume, and number of intersections, as well as significantly decreased branch volume and branch length inside the phrenic motor nucleus. Additionally, astrocytic number in the non-phrenic motor nucleus region immediately outside of the phrenic motor nucleus was significantly increased while branch length was significantly decreased in CTB-SAP treated rats. Collectively, these findings are indicative of a reactive morphological state in the phrenic motor nucleus in CTB-SAP rats and suggest that astrocytes may also be an origin of TNF-α to impact phrenic plasticity and ultimately breathing.

Usage: Intrapleural injection of CTB-SAP (IT-14) in rat

Related Products: CTB-SAP (Cat. #IT-14)

Keilholz A, Henry J, Osman K, Smith C, Haxton C, Ozden I, Lever T, Nichols N (2025) Impact of TrkB receptor modulation on tongue exercise-induced plasticity in an inducible rodent model of hypoglossal (xii) motor neuron death. Am Physiol Soc 40(S1) doi: 10.1152/physiol.2025.40.S1.0788

Objective: Authors hypothesized that the source of tongue exercise-induced plasticity in CTB-SAP rats arises from a BDNF- dependent pathway to preserve upper airway function.

Summary: Authors targeted tropomyosin receptor kinase B (TrkB) by delivery of either a TrkB receptor antagonist (ANA-12) or vehicle (veh; 50:50 DMSO+aCSF) into the XII nucleus of the brainstem in adult male rats for the following groups: 1) control+sham exercise+veh 2) CTB-SAP+exercise+veh 3)CTB-SAP+exercise+ANA-12. The effect of TrkB receptor inhibition on tongue exercise-induced plasticity was assessed by conducting behavioral assays. The findings suggest that CTB-SAP+exercise+ANA-12 rats have decreased lick force/rate vs. CTB-SAP+/-exercise+/-veh rats as well as control +/-exercise+/-veh rats.

Usage: Intralingual injection of CTB-SAP (IT-14) in rat

Related Products: CTB-SAP (Cat. #IT-14)

Oti GO, Basak A, Eason G, Kington S, Drago C, Smith L, Osman K, Smith CL, Keilholz A, Lever TE, Nichols NL (2025) Therapeutic potential of tongue exercise on tongue force/strength during swallowing in a rodent model of hypoglossal (xii) motor neuron loss. Am Physiol Soc 40(S1) doi: 10.1152/physiol.2025.40.S1.1188

Objective: To develop a rodent model of XII axis dysfunction by administering CTB-SAP to explore potential treatment for neuromuscular disorders.

Summary: The data demonstrate that the number of evoked swallows did not differ between groups, and threshold stimulation elicited fewer swallows compared to suprathreshold intensities.

Usage: Adult male rats were intralingually injected with either 25 ug CTB-SAP (IT-14) or control (CTB unconjugated to SAP)

Related Products: CTB-SAP (Cat. #IT-14)

Kozlova E, Lam A, Alam S, Shum J, Denys M, Berdasco C, de-Lartigue G, Curras-Collazo M (2025) Do CCKA receptor containing vagal afferent neurons mediate gut-brain inflammatory signals?. Am Physiol Soc 40(S1) doi: 10.1152/physiol.2025.40.S1.2035

Objective: To investigate whether CCKA receptor, containing vagal afferent neurons (VANs), mediate gut-brain inflammatory signaling and contribute to the systemic immune response following an LPS challenge.

Summary: This abstract reports that ablation of CCKAR+ VANs via CCK-SAP reduced LPS-induced IL-6 levels and brain c-Fos expression, indicating a blunted inflammatory response. These findings support the role of CCKAR+ VANs in gut-brain immune signaling and their potential as therapeutic targets.

Usage: CCK-SAP (IT-31) was bilaterally injected into the nodose ganglia (250 nL, 250 ng/μL) to ablate CCKA receptor–expressing vagal afferents.

Related Products: CCK-SAP (Cat. #IT-31)

Janes TA, Parra Sanchez AS, Cardani S, Pagliardini S (2025) Progestin infusion into the hypothalamus of CO2-chemoreflex impaired female rats enhances the hypercapnic ventilatory response. Am Physiol Soc 40(S1) doi: 10.1152/physiol.2025.40.S1.1430

Objective: Authors hypothesized that etonogestrel (ETO) infusion into the dorsomedial hypothalamus (DMH) would elicit CO2-chemo reflex recovery similar to that observed in systemically treated female rats.

Summary: The hypothesis was tested by impairing the CO2-chemoreflex in adult female rats by lesioning retrotrapezoid nucleus (RTN) neurons with substance P-conjugated saporin toxin. Preliminary data suggest that ETO induced modest CO2-chemoreflex recovery in rats with moderate-sized RTN lesion by increasing tidal volume and breathing frequency, while hypoxic ventilation was unaffected. In rats with large RTN lesion, no restorative effect was observed. These data suggest a potential ETO-induced compensatory role for DMH neurons following central CO2-chemoreflex impairment but do not rule out contributions from other brainstem nuclei based on previous systemic ETO administration.

Usage: The CO2-chemoreflex was impaired in adult female Sprague-Dawley rats by lesioning RTN neurons with substance P-saporin toxin (SP-SAP).

Related Products: SP-SAP (Cat. #IT-07)

Shibata Y, Matsumoto Y, Kohno K, Nakashima Y, Tsuda M (2025) Microgliosis in the spinal dorsal horn early after peripheral nerve injury is associated with damage to primary afferent aβ-fibers. Cells 14(9):666. doi: 10.3390/cells14090666 PMID: 40358190

Objective: To investigate the spatial relationship between microgliosis and the projections of injured nerves by generating mice that had expressed tdTomato in the fourth lumbar dorsal root ganglion (L4-DRG) neurons via intra-L4-spinal nerve (SpN) injection of adeno-associated viral vectors.

Summary: After transection of the L4-SpN, authors found that microgliosis in the SDH selectively occurred in the innervation territories of the injured primary afferent fibers. However, denervating transient receptor potential vanilloid 1 (TRPV1)-expressing primary afferent fibers in theSDH through intrathecal injection of capsaicin did not trigger microgliosis, nor did it influence the microgliosis induced by L4-SpN injury. Conversely, pharmacological damage to myelinated DRG neurons, including Aβ-fibers, was sufficient to induce microgliosis. Furthermore, L4-SpN injury also induced microgliosis in the gracile nucleus, which primarily receives innervation from Aβ-fibers. These findings suggest that microgliosis in the SDH shortly after peripheral nerve injury is predominantly associated with damage to primary afferent Aβ-fibers.

Usage: WT mice were injected with saporin into the L4-SpN, administering 400 nL of saporin solution [saporin conjugated with cholera toxin B subunit (CTB-SAP), isolectin B4 (IB4-SAP),and unconjugated saporin (Ctrl-SAP)]

Related Products: CTB-SAP (Cat. #IT-14), IB4-SAP (Cat. #IT-10), Saporin (Cat. #PR-01)

Kiyama T, Chen CK, Altay HY, Chen YJ, Sigala L, Su D, Eliason S, Amendt BA, Mao CA (2025) Tbr2-dependent parallel pathways regulate the development of distinct ipRGC subtypes. bioRxiv 2025.04.29.651262. doi: 10.1101/2025.04.29.651262

Objective: To demonstrate that two Tbr2-dependent transcription factors, Iroquois‑related homeobox 1 (Irx1) and T-box containing factor 20 (Tbx20), are key downstream transcription factors guiding lineage segregations of Tbr2-expressing RGC into distinct adult intrinsically photo sensitive retinal ganglion cells (ipRGC) subtypes.

Summary: Both transcription factors, Irx1 and Tbx20, also control Opn4 expression. When Irx1 is ablated during retinal development, Opn4 expression is significantly reduced in the M3, M4, and M5 ipRGC groups; however, the formation of Irx1-expressing ipRGCs is not affected. In contrast, when Tbx20 is deleted, a significant number of Tbx20-expressing cells fail to develop while Opn4 expression is down-regulated. These findings reveal two parallel transcription cascades downstream of Tbr2 for controlling ipRGC subtype formation, fate divergence, and maintenance in the adult retina.

Usage: Retinal sections or flat-mounted retinas were fixed with 4% paraformaldehyde and then incubated with the Anti-Melanopsin (AB-N39).

Related Products: Melanopsin Rabbit Polyclonal, affinity-purified (Cat. #AB-N39)

Hung LY, Alves ND, Del Colle A, Talati A, Najjar SA, Bouchard V, Gillet V, Tong Y, Huang Z, Browning KN, Hua J, Liu Y, Woodruff JO, Juarez D, Medina M, Posner J, Tonello R, Yalcinkaya N, Israelyan N, Ringel R, Yang L, Leong KW, Yang M, Sze JY, Savidge T, Gingrich J, Shulman RJ, Gershon MD, Ouellet A, Takser L, Ansorge MS, Margolis KG (2025) Intestinal epithelial serotonin as a novel target for treating disorders of gut-brain interaction and mood. Gastroenterology 168(4):754-768. doi: 10.1053/j.gastro.2024.11.012 PMID: 39672518

Objective: To investigate how intestinal epithelial serotonin influences mood and gastrointestinal function, and to identify gut-targeted therapies for mood disorders and disorders of gut-brain interaction (DGBI).

Summary: Selective deletion of the serotonin transporter (SERT) from the intestinal epithelium reduced anxiety- and depression-like behaviors in mice without affecting gut motility or cognition. These effects were dependent on afferent vagal signaling. Conversely, depleting intestinal serotonin increased anxiety. A human birth cohort study linked in utero SSRI/SNRI exposure to a higher risk of functional constipation, supporting a gut-brain role in DGBI.

Usage: CCK-SAP (IT-31) was bilaterally injected into the nodose ganglia, and after one week, tests of anxiety and depression were performed.

Related Products: CCK-SAP (Cat. #IT-31)

Ren L, Zhao Z, Chao Y, Yu P, Mei Z, Du B, Cheng Y (2025) Optimization of lipid nanoparticles with robust efficiency for the delivery of protein therapeutics to augment cancer immunotherapy. Adv Sci (Weinh) e2500844. doi: 10.1002/advs.202500844 PMID: 40056044

Objective: To develop a family of Lipid Nanoparticles (LNPs) with robust high efficiency in addressing the multiple barriers in cytosolic protein delivery by incorporating clinically approved ionizable lipids into traditional cationic LNPs. The authors investigated the in vivo delivery efficacy of the LNPs using saporin as the model protein.

Summary: The combination of cationic and ionizable lipids enables efficient protein binding and endosomal escape. Optimized LNPs efficiently deliver various proteins, including antibodies, enzymes, toxins, and Cas9 into living cells with reserved functions. The optimized LNPs successfully deliver therapeutic proteins such as saporin and interleukin-10 (IL-10) to inhibit tumor growth in several animal models.

Usage: Cell Viability and Apoptosis Assay: For evaluating in vitro saporin delivery efficacy, 143B cells were incubated with free saporin or saporin/LNP at saporin concentrations ranging from 0 to 61 nm. In Vivo Saporin Delivery by LNP: mice were intravenously injected with 100μL saporin or saporin/LNP once every two days. The doses of saporin and LNP in in vivo experiments were fixed at 50μg kg−1 and 2.4 mg lipid/kg, respectively.

Related Products: Saporin (Cat. #PR-01)