IB4-SAP [IT-10]

a tool for eliminating cells that express alpha-D-galactopyranoside residues in cells; targeted via recombinant Isolectin B4 (IB4), eliminated via saporin

SKU: IT-10 Category: Product Size: 25 ug, 100 ug, 250 ug, 1 mg | Host: E. coli | Conjugate: saporin | Usage: eliminates cells |

Isolectin B4 (IB4) is one of a family of five alpha-D-galactose-binding lectins from Griffonia (Bandeiraea) simplicifolia. Recombinant IB4* was expressed in E. coli and purified using affinity chromatography. In one important application rIB4-SAP specifically eliminates the IB4-positive c-fiber nociceptor neurons, while sparing the peptidergic neurons. Upon binding the alpha-D-galactopyranoside residues expressed on the cell surface, rIB4-SAP becomes internalized and saporin inhibits protein synthesis, resulting in elimination of the neurons. The cytotoxin is extremely potent, with an ED50 in the low picomolar range for some alpha-D-galactopyranoside-expressing cells in vitro. rIB4-SAP is an excellent tool for the study of pain transmission and the biological roles of IB4+ cells in vivo.

*patent pending

IB4-SAP eliminates a-D-galactosyl-positive cells. All other cells are left untouched.

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