Isolectin B4 (IB4) is one of a family of five alpha-D-galactose-binding lectins from Griffonia (Bandeiraea) simplicifolia. These isolectins are tetromeric structures, consisting of various combinations of A and B subunits. Recombinant IB4*, unlike the native form, can have no contamination from the A subunit. IB4 has been used to label primary sensory afferent terminals in the rat dorsal horn, inhibit ascites tumor cell growth, label small-diameter trigeminal ganglion neurons, and to agglutinate B-type red blood cells.
* patent pending
This recombinant protein binds terminal alpha-D-galactose groups. Recombinant IB4 was expressed in E. coli and purified using affinity chromatography. This product is routinely tested by coomassie stain of an SDS-PAGE gel and agglutination assay.
Applications include red blood cell (RBC) agglutination.
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