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Inflammatory macrophages facilitate mechanical stress-induced osteogenesis.
Zhang F, Huan L, Xu T, Li G, Zheng B, Zhao H, Guo Y, Shi J, Sun J, Chen A (2020) Inflammatory macrophages facilitate mechanical stress-induced osteogenesis. Aging (Albany NY) 12(4):3617-3625. doi: 10.18632/aging.102833
Summary: In a mouse model of distraction osteogenesis (DO), there was significant increase in macrophages in the regeneration area. This suggests that targeting inflammatory macrophages may help to improve clinical bone repair.
Usage: For saporin-mediated depletion of macrophages, DO-surgery-treated mice received an intraventricular (iv) injection of either Mac-1-SAP or Rat IgG-SAP (20µg) once every 3 days.
Related Products: Mac-1-SAP mouse/human (Cat. #IT-06), Rat IgG-SAP (Cat. #IT-17)
Fugan Wan alleviates hepatic fibrosis by inhibiting ACE/Ang II/AT-1R signaling pathway and enhancing ACE2/Ang 1-7/Mas signaling pathway in hepatic fibrosis rat models
Li S, Zhao W, Tao Y, Liu C (2020) Fugan Wan alleviates hepatic fibrosis by inhibiting ACE/Ang II/AT-1R signaling pathway and enhancing ACE2/Ang 1-7/Mas signaling pathway in hepatic fibrosis rat models. Am J Transl Res 12(2):592-601. PMID: 32194907
Objective: To investigate protective effects of Fugan Wan (FGW) on hepatic fibrosis and clarify associated mechanisms.
Summary: FGW alleviated hepatic fibrosis by inhibiting ACE/Ang II/AT-1R signaling and enhancing ACE2/Ang 1-7/Mas signaling pathway in hepatic fibrosis rat models.
Usage: Western blot
Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP)
Intervening B-type natriuretic peptide signaling for controlling chronic itch.
Meng J, Chen W, Wang J (2020) Intervening B-type natriuretic peptide signaling for controlling chronic itch. Brit J Pharmacol 177(5):1025-1040. doi: 10.1111/bph.14952
Objective: Review of recent findings used to examine the role of B-type natriuretic peptide (BNP) in itch transduction and the modulation of other pururitic proteins.
Summary: Mice treated with Nppb-SAP ablated 70% of the BNP receptor-positive neurons in the spinal cord.
Related Products: Nppb-SAP (Cat. #IT-69)
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Voltage-gated calcium channel subunit α2δ-3 shapes light responses of mouse retinal ganglion cells mainly in low and moderate light levels
Seitter H, Sothilingam V, Benkner B, Garrido MG, Kling A, Pirone A, Seeliger M, Münch TA (2020) Voltage-gated calcium channel subunit α2δ-3 shapes light responses of mouse retinal ganglion cells mainly in low and moderate light levels. bioRxiv 2020.02.10.941807. doi: 10.1101/2020.02.10.941807
Related Products: Melanopsin Rabbit Polyclonal, affinity-purified (Cat. #AB-N39)
nGnG amacrine cells and Brn3b-negative M1 ipRGCs are specifically labeled in the ChAT-ChR2-EYFP mouse.
Cui LJ, Chen WH, Liu AL, Han X, Jiang SX, Yuan F, Zhong YM, Yang XL, Weng SJ (2020) nGnG amacrine cells and Brn3b-negative M1 ipRGCs are specifically labeled in the ChAT-ChR2-EYFP mouse. Invest Ophthalmol Vis Sci 61(2):14. doi: 10.1167/iovs.61.2.14 PMID: 32049344
Summary: The authors speculated that type II cells might be ipRGCs. This was later verified by the strong immunostaining of type II cells in response to the melanopsin antibody UF006 (100%, 141 of 141 cells collected from 5 retinas, Figs. 6B1–B3), which probes multiple ipRGC subtypes.
Usage: Immunostaining 1:10000
Related Products: Melanopsin Rabbit Polyclonal (Cat. #AB-N38)
Cell lineage tracing identifies hormone-regulated and wnt-responsive vaginal epithelial stem cells
Ali A, Syed SM, Jamaluddin MFB, Colino-Sanguino Y, Gallego-Ortega D, Tanwar PS (2020) Cell lineage tracing identifies hormone-regulated and wnt-responsive vaginal epithelial stem cells. Cell Rep 30(5):1463-1477.e7. doi: 10.1016/j.celrep.2020.01.003 PMID: 32023462
Objective: To learn more about the role of Wnt signaling in vaginal epithelium (VE) homeostasis.
Summary: Data define heterogeneity in VE and identify VE stem cells. It was shown that canonical Wnt/b-catenin signaling is required for the proliferation and differentiation of VE stem cells.
Usage: histology and immunostaining (1:250)
Related Products: NGFr (mu p75) Rabbit Polyclonal (Cat. #AB-N01)
Quantum dot conjugated saporin activates microglia and induces selective substantia nigra degeneration.
Landrigan J, Dwyer Z, Beauchamp S, Rodriguez R, Fortin T, Hayley S (2020) Quantum dot conjugated saporin activates microglia and induces selective substantia nigra degeneration. NeuroToxicology 76:153-161. doi: 10.1016/j.neuro.2019.11.007 PMID: 31738977
Objective: To assess the impact of Quantum Dots (QDs) alone and QDs conjugated to Saporin, on substantia nigra microglia and dopamine neurons.
Summary: QDs might be a viable route for toxicant delivery and also have an added advantage of being fluorescently visible. Ultimately, we found SNc neurons to be exceptionally vulnerable to QD-saporin and suggest that this could be a novel targeted approach to model Parkinson’s Disease-like inflammatory pathology.
Usage: Biotin-labeled saporin chicken polyclonal was mixed with QDs coated with streptavidin. 2 μl of QDs (1 μM) were mixed with 2 μL of biotinylated saporin (56 μM) and 76 μL of phosphate buffer solution was added.
Related Products: Saporin Chicken Polyclonal, affinity-purified biotin-labeled (Cat. #AB-17APBT)
More than scratching the surface: recent progress in brain mechanisms underlying itch and scratch.
Liu X, Miao XH, Liu T (2020) More than scratching the surface: recent progress in brain mechanisms underlying itch and scratch. Neurosci Bull 36(1):85-88. doi: 10.1007/s12264-019-00352-1
Summary: The discovery of descending neural circuitry to drive the itch-scratching cycle may provide potential therapeutic targets in the central nervous system for the management of chronic itch.
Usage: To ablate the spinal GRPR+ neurons, mice were intrathecally injected with Bombesin-SAP or Blank-SAP (400 ng/5 mL).
Related Products: Bombesin-SAP (Cat. #IT-40)
Time and region-dependent manner of increased brain derived neurotrophic factor and TrkB in rat brain after binge-like methamphetamine exposure
Moreira da Silva Santos A, Gorman AM, Kelly JP, Doyle KM (2020) Time and region-dependent manner of increased brain derived neurotrophic factor and TrkB in rat brain after binge-like methamphetamine exposure. Neurosci Lett 715:134606. doi: 10.1016/j.neulet.2019.134606 PMID: 31693929
Objective: To investigate the effect of binge-like methamphetamine (MA) dosing on brain-derived neurotrophic factor (BDNF) levels and its receptors, trkB and p75NTR.
Summary: The binge-like regimen of MA affects expression of BDNF and its receptors, particularly the trkB receptor, in a time and region dependent manner, and highlights the importance of the frontal cortex and the striatum in the response following MA binge-like dosing.
Usage: Western blot (1:1000) NGFr (mu p75) Rabbit Polyclonal, affinity-purified
Related Products: NGFr (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)
ALPPL2 is a highly specific and targetable tumor cell surface antigen
Su Y, Zhang X, Bidlingmaier S, Behrens CR, Lee NK, Liu B (2020) ALPPL2 is a highly specific and targetable tumor cell surface antigen. Cancer Res 80(20):4552-4564. doi: 10.1158/0008-5472.CAN-20-1418 PMID: 32868383
Objective: To evaluate therapeutic potential of ALPPL2 targeting.
Summary: Exquisite tissue specificity and broad tumor type coverage suggest that ALPPL2 could be an excellent cell surface target for therapeutic development against mesothelioma.
Usage: Biotinylated M25 IgG1 and Streptavidin-ZAP were mixed at a molar ratio of 1:1.
Related Products: Streptavidin-ZAP (Cat. #IT-27)