Liu AL, Liu YF, Wang G, Shao YQ, Yu CX, Yang Z, Zhou ZR, Han X, Gong X, Qian KW, Wang LQ, Ma YY, Zhong YM, Weng SJ, Yang XL (2022) The role of ipRGCs in ocular growth and myopia development. Sci Adv 8(23):eabm9027. doi: 10.1126/sciadv.abm9027 PMID: 35675393
Objective: To provide evidence in a mouse model to demonstrate that intrinsically photosensitive retinal ganglion cells (ipRGCs) make a considerable contribution to the establishment of the ocular refractive system.
Summary: The involvement of ipRGCs in mouse refractive development was investigated with selective ipRGC ablation using Melanopsin-SAP. The authors found that ipRGCs are substantially involved in ocular refractive development in mice, which is crucial for image-forming visual functions. This conclusion is derived from the considerable myopic refractive shifts induced in the eyes when most ipRGCs were ablated, yet they exhibited hyperopic shifts when ipRGCs were selectively activated.
Usage: C57BL/6 mice received intravitreal injection of Melanopsin-SAP (IT-44) at three concentrations [200, 400, and 800 ng, all suspended in 0.75 µl of 0.1 M PBS (pH 7.4)] at postnatal day 18; control eyes received the same volume of PBS or 400 ng of nonspecific antibody–conjugated saporin (IgG-SAP, also in 0.75 µl of PBS).
Related Products: Melanopsin-SAP (Cat. #IT-44), Rabbit IgG-SAP (Cat. #IT-35), Melanopsin Rabbit Polyclonal, affinity-purified (Cat. #AB-N39)