References

Heck AJ (2023) Chemical design of a supramolecular protein nanoplatform for therapeutic applications. Univ Johannes Gutenberg Thesis.

Objective: Investigate the potential of using multivalent proteins as an integrative nanoplatform to target protein hallmarks in various diseases by forming supramolecular protein conjugates (SPC).

Summary: The author employs an integrative and adaptable protein-based nanoplatform to combine bioactive entities at the macromolecular level to design multivalent protein-conjugates for versatile therapeutic purposes. Multivalent protein-conjugates, a tetrameric protein with up to four ligand binding sites functions is used as a supramolecular “glue” utilizing avidin-biotin technology. The study investigates the assembly of diverse bioactive components into well organized multifunctional protein-conjugates, ultimately leading to the formation of supramolecular protein conjugates (SPC) that target protein signatures in a variety of diseases.

Usage: Streptavidin-ZAP with Anti-PSMA Antibody

Related Products: Streptavidin-ZAP (Cat. #IT-27)

See Also:

• Kuroda K et al. Saporin toxin-conjugated monoclonal antibody targeting prostate-specific membrane antigen has potent anticancer activity. Prostate 70(12):1286-1294, 2010.

Loftén A, Adermark L, Ericson M, Söderpalm B (2023) Regulation of ethanol-mediated dopamine elevation by glycine receptors located on cholinergic interneurons in the nucleus accumbens. Addict Biol 28(12):e13349. doi: 10.1111/adb.13349 PMID: 38017639

Objective: The aim of this study was to explore the role of glycine receptors (GlyRs) on cholinergic interneurons (CIN) in sustaining extracellular dopamine levels and in ethanol-induced dopamine release.

Summary: Alcohol use disorder is one of the major psychiatric disorders worldwide. Ethanol reward is one of the many factors contributing to the disorder. The rewarding and reinforcing properties of ethanol have been linked to activation of the mesolimbic dopamine system, an effect that involves glycine receptors (GlyRs) in the nucleus accumbens. The study suggests that CIN are not important for GlyR-mediated regulation of basal dopamine output, but that CIN ablation blunts the ethanol-induced dopamine release by reducing the release of GlyR agonists.

Usage: CIN were ablated by Anti-ChAT-SAP administered locally in the nucleus accumbens of male Wistar rats. Rabbit-IgG-SAP was used as a control. Microinfusion was performed unilaterally into the nAc at a concentration of 0.5 ug/ul at 0.05 ul/min for 10 min for a total of 0.5 ul.

Related Products: Anti-ChAT-SAP (Cat. #IT-42), Rabbit IgG-SAP (Cat. #IT-35)

Takanami K, Kuroiwa M, Ishikawa R, Imai Y, Oishi A, Hashino M, Shimoda Y, Sakamoto H, Koide T (2023) Function of gastrin-releasing peptide receptors in ocular itch transmission in the mouse trigeminal sensory system. Front Mol Neurosci 16:1280024. doi: 10.3389/fnmol.2023.1280024 PMID: 38098939

Objective: To investigate the role of gastrin-releasing peptide (GRP) and GRP receptor (GRPR) in itch transmission in the spinal somatosensory system, and to determine whether the GRP system is involved in itch neurotransmission of the eyes in the trigeminal sensory system

Summary: Administering itch mediators like histamine (His) and chloroquine (CQ) caused high levels of eye scratching in a concentration-dependent manner, with significant gender differences observed for His. Histological studies showed that His and CQ significantly activated GRPR-expressing neurons in a specific brain region of transgenic mice. Blocking these neurons with a GRPR antagonist or eliminating them reduced CQ-induced scratching. Injecting a GRPR agonist without an itch stimulus led to excessive facial scratching, indicating the central role of GRPR neurons in mediating itch responses.

Usage: 500 ng Blank-SAP (IT-21) or 500 ng Bombesin-SAP (IT-40) were intracisternally administered (5-uL volume) 2 weeks prior to behavioral experiments.

Related Products: Bombesin-SAP (Cat. #IT-40), Blank-SAP (Cat. #IT-21)

Singh RR, Mondal I, Janjua T, Popat A, Kulshreshtha R (2023) Engineered smart materials for RNA based molecular therapy to treat Glioblastoma. Bioact Mater 33:396-426. doi: 10.1016/j.bioactmat.2023.11.007 PMID: 38059120

Objective: A review of non-coding RNA therapy and its targeted delivery of nucleic acids to treat Glioblastoma, emphasizing smart nano-materials.

Summary: Nano-carriers of ncRNA can offer unique advantages in fighting, such as low cytotoxicity, ability to cross the blood-brain barrier, stealth to bypass immune detection, prolonged release of the cargo, improved circulatory time, and also targeted therapy.

Usage: Saporin as a payload to the nano-carriers angiopep-2 peptide and RAP12.

Related Products: Saporin (Cat. #PR-01)

Chamberlain AR, Huynh L, Huang W, Taylor DJ, Harris ME (2023) The specificity landscape of bacterial ribonuclease P. J Biol Chem 300(1):105498. doi: 10.1016/j.jbc.2023.105498 PMID: 38013087

Objective: Review of the specificity of ribonucleoprotein RNase P in binding different types of RNA.

Summary: Ribonucelase P is involved in the RNA metabolism pathways. By studying the rate at which it combines with different types of RNA under different conditions, like concentration and competition with different enzymes, a model describing its specificity to different RNA motifs can be developed.

Related Products: Saporin (Cat. #PR-01)

See Also:

• Hauf, S., Rotrattanadumrong, R., and Yokobayashi, Y. (2022) Analysis of the sequence preference of saporin by deep sequencing. ACS Chem. Biol.17, 2619–2630

Kirkey DC, Robinson L, Janssens D, Hines MG, Hylkema T, Manselle MK, Ries RE, Peplinski JH, Wallace LK, Otto D, Tarlock K, Henikoff S, Li W, Meshinchi S (2023) CLEC2A is a novel AML-restricted immunotherapeutic target enriched in KMT2A-rearranged acute myeloid leukemia. Blood 142(1):290. doi: 10.1182/blood-2023-188439

Objective: Targeting CLEC2A, a novel immunotherapeutic target, in the treatment of acute myeloid leukemia.

Summary: CLEC2A, a novel immunotherapeutic target expressed highly in acute myeloid leukemia, is a potential target in treating leukemia. By conjugating an antibody targeting this protein to saporin, an ADC for the treatment of leukemia can be created.

Usage: A CLEC2A-antibody was conjugated to Hum-ZAP [IT-22] and treated against CLEC2A+ and CLEC2A- cells and target-specific cytotoxicity was observed at 10nM levels.

Related Products: Hum-ZAP (Cat. #IT-22)

Konturek-Ciesla A (2023) Aging hematopoiesis: Functional insights and prospects for rejuvenation. Lund University Thesis.

Objective: To examine the function of hematopoietic stem cells (HSCs), the source of all mature blood cells, during adulthood and upon aging.

Summary: A gradual decrease in HSCs multi-lineage differentiation capacity, with the most significant reduction in their lymphoid output. The decreased lymphopoiesis could be attributed to the lymphoid progenitor subset, MPP Ly-I, revealing defects in the HSCs transition to these cells. Hematopoietic rejuvenation techniques studied through HSCs and their aging processes.

Usage: Anti-CD45-SAP (IT-91) was delivered to ex vivo HSCs cells.

Related Products: Anti-CD45.2-SAP (Cat. #IT-91)

Zlatkovic J, Dalmau Gasull A, Hägg D, Font-Gironès F, Bellman J, Meister B, Palsdottir V, Ruud J, Ohlsson C, Dickson SL, Anesten F, Jansson JO (2023) Reduction of body weight by increased loading is associated with activation of norepinephrine neurones in the medial nucleus of the solitary tract. J Neuroendocrinol 35(12):e13352. doi: 10.1111/jne.13352 PMID: 37885347

Objective: To show that the feed-back regulation of body weight, initiated by a novel leptin-independent body weight homeostat, involves a CNS mechanism

Summary: In conclusion, increased load appears to reduce body weight and food intake via activation of norepinephrine neurones in the medial nucleus of the solitary tract.

Usage: 5 ng of Anti-DBH-SAP (IT-03) was bilaterally injected into the NTS in Male C57/B6J mice and Sprague–Dawley rats.

Related Products: Anti-DBH-SAP (Cat. #IT-03)

Makarova AO, Kostenk VV, Ovsyanikova OV, Svirshchevskaya EV, Lutsenko GV, Lutsenko AM (2023) Heat shock proteins on tumor cell surface as target for anti-tumor therapy (a review). Russian Journal of Bioorganic Chemistry 50(3):644-656. doi: 10.1134/S1068162024030038

Objective: To present the characteristics of the main proteins of the heat shock proteins (HSP) family, the features of their expression in tumor cells, and the possibility of using monoclonal antibodies against these proteins as a guiding vector for anti-tumor immunotherapy.

Summary: Apart from targeted delivery of NPs and different therapeutic drugs into cancer cells, another advantage of mAbs against HSP70 is their ability to activate anti-tumor antibody-dependent cellular cytotoxicity.

Usage: Certain antitumor drugs can be delivered by mAbs not only as part of NPs, but also as a drug–antibody conjugate. For instance, anti-HSP65 mouse mAb ML30 conjugated to Saporin (PR-01) almost fully inhibited cell proliferation of cell lines U937 and Daudi that express HSP65 on their surfaces.

Related Products: Saporin (Cat. #PR-01), Custom Conjugates

Gamage R, Rossetti I, Niedermayer G, Münch G, Buskila Y, Gyengesi E. (2023) Chronic neuroinflammation during aging leads to cholinergic neurodegeneration in the mouse medial septum. J Neuroinflammation 20(1):235. doi: 10.1186/s12974-023-02897-5 PMID: 37833764

Summary: This publication presents the findings of studies on age-related cholinergic decline. The authors mention a diffusion MRI study that documented a 25% reduction in the BFCN (Basal Forebrain Cholinergic Neurons) population and a significant loss of terminal cholinergic projections in the hippocampus after inducing selective BFCN degeneration in mice using mu p75-SAP.

Related Products: mu p75-SAP (Cat. #IT-16)

See Also:

• Kerbler GM et al. Diffusion-weighted magnetic resonance imaging detection of basal forebrain cholinergic degeneration in a mouse model. Neuroimage 66C:133-141, 2013.