Blank-Streptavidin-SAP [IT-27B]

a tool for use as control with streptavidin-saporin conjugates; non-targeted via non-specific biotin-tagged peptide conjugated to streptavidin-saporin

SKU: IT-27B Category: Quantity: 25 ug, 100 ug, 250 ug | Reactivity: no known specificity | Conjugate: streptavidin-saporin | Usage: control |

Blank-Strep-SAP is the perfect control for use with peptide conjugates utilizing biotin linkage to Streptavidin-ZAP (IT-27). It consists of a non-targeted peptide that has been biotinylated and mixed with Streptavidin-ZAP. The sequence of the non-targeted peptide of this molecule is an 11-amino acid, randomly mixed version of the sequence of melanocyte-stimulating hormone, with amino acid residues that are typical of peptides that bind to G-protein-coupled receptors. Examination of the peptide sequence using NCBI/BLAST reveals no homologous sequences. Thus, Blank-Strep-SAP can be used as control for any non-specific effects of the toxin and provides a definitive baseline for determining the effects of a targeted peptide conjugate.

Blank-Strep-SAP serves as a control for peptide-targeted toxins utilizing biotin-streptavidin linkage.

keywords: saporin, control, peptide, blank, streptavidin, biotin, Blank-Streptavidin-SAP, Blank-Strep-SAP


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Cytotoxicity Assay Protocol for Control Conjugates

Online Calculator: Preparing Control Conjugate Samples

Concentration Calculations: Convert molarity to mg/ml and mg/ml to molarity (PDF worksheet)

Preparing and Interpreting Cytotoxicity Data in vitro

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