- Home
- Knowledge Base
- References
References
Reciprocal interactions between osteoclasts and nociceptive sensory neurons in bone cancer pain
Andriessen AS, Donnelly CR, Ji RR (2021) Reciprocal interactions between osteoclasts and nociceptive sensory neurons in bone cancer pain. Pain Rep 6(1):e867. doi: 10.1097/PR9.0000000000000867
Summary: Current pharmacotherapies available for bone cancer pain are insufficient to provide safe and efficacious pain relief. The authors discuss the mechanisms used by cancer cells within the bone tumor microenvironment (TME) to drive bone cancer pain.
Usage: Microglial ablation using Mac-1-SAP (15 μg in 8.8 μl i.t.) and Saporin control (Cat. #PR-01, 8.8 μg in 8.8 μl), is sufficient to attenuate nerve injury-induced pain in male, but not female mice.
Related Products: Mac-1-SAP mouse/human (Cat. #IT-06), Saporin (Cat. #PR-01)
Integrin α10-antibodies reduce glioblastoma tumor growth and cell migration
Masoumi KC, Huang X, Sime W, Mirkov A, Munksgaard Thorén M, Massoumi R, Lundgren-Åkerlund E (2021) Integrin α10-antibodies reduce glioblastoma tumor growth and cell migration. Cancers (Basel) 13(5):1184. doi: 10.3390/cancers13051184
Summary: The authors investigated the treatment effect of two antibodies that have been developed to target the protein integrin 10, which is present on the surface of Glioblastoma (GB) cells. Function-blocking integrin alpha10, beta1-antibodies inhibit GB tumor growth as well as the migration of GB cells. This further validates integrin alpha10, beta1 as a promising target in GB and suggests a novel therapeutic strategy for the treatment of GB and other high-grade gliomas.
Usage: Infusions of anti-integrin α10β1-SAP or Anti-ctrl-SAP were made icv (1 µg/2 L per infusion).
Related Products: Custom Conjugates
Fndc5 knockdown significantly decreased the expression of neurotrophins and their respective receptors during neural differentiation of mouse embryonic stem cells
Ebadi R, Rabiee F, Kordi-Tamandani D, Nasr-Esfahani MH, Ghaedi K (2021) Fndc5 knockdown significantly decreased the expression of neurotrophins and their respective receptors during neural differentiation of mouse embryonic stem cells. Hum Cell 34(3):847-861. doi: 10.1007/s13577-021-00517-z PMID: 33683654
Objective: To evaluate whether knockdown of Fibronectin type III domain-containing-5 (Fndc5) decreases neural differentiation of mouse embryonic stem cells (mESCs) by affecting the neurotrophins and their receptor expression.
Summary: Results suggest that decreased efficiency of neural differentiation following the reduction of Fndc5 expression could be attributed to decreased levels of NGF and BDNF proteins in addition to their cognate receptors.
Usage: Western blot (1:4000)
Related Products: NGFr (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)
Minocycline in neurodegenerative and psychiatric diseases: An update.
Romero-Miguel D, Lamanna-Rama N, Casquero-Veiga M, Gómez-Rangel V, Desco M, Soto-Montenegro ML (2021) Minocycline in neurodegenerative and psychiatric diseases: An update. Eur J Neurol 28(3):1056-1081. doi: 10.1111/ene.14642
Summary: Review includes the mouse animal model of neurodegenerative disease using mu p75-SAP. Biological Effects: Attenuation of cholinergic neurons loss, glial activation and transcription of pro-inflammatory mediators.
Usage: 45 mg/Kg, i.p.
Related Products: mu p75-SAP (Cat. #IT-16)
Tongue and hypoglossal morphology after intralingual cholera toxin B-saporin injection
Lind LA, Lever TE, Nichols NL (2021) Tongue and hypoglossal morphology after intralingual cholera toxin B-saporin injection. Muscle Nerve 63(3):413-420. doi: 10.1002/mus.27131
Objective: To evaluate tongue morphology and ultrastructural changes in hypoglossal neurons and nerve fibers in an inducible rat model of dysphagia.
Summary: Preliminary results indicate this model may have translational application to a variety of neurodegenerative diseases resulting in tongue dysfunction and associated dysphagia.
Usage: Rats assigned to the CTB-SAP group (n = 10) received 25 μg CTB-SAP to produce hypoglossal motor neuron death.
Related Products: CTB-SAP (Cat. #IT-14)
Intact vagal gut-brain signalling prevents hyperphagia and excessive weight gain in response to high-fat high-sugar diet.
McDougle M, Quinn D, Diepenbroek C, Singh A, de la Serre C, de Lartigue G (2021) Intact vagal gut-brain signalling prevents hyperphagia and excessive weight gain in response to high-fat high-sugar diet. Acta Physiol (Oxf) 231(3):e13530. doi: 10.1111/apha.13530
Objective: To assess the function of the vagus nerve lack specificity.
Summary: Intact sensory vagal neurons prevent hyperphagia and exacerbation of weight gain in response to a HFHS diet by promoting lipid-mediated satiation.
Usage: Rat nodose ganglia were injected bilaterally with either CCK-SAP or unconjugated saporin as a control.
Related Products: CCK-SAP (Cat. #IT-31)
Activated factor X targeted stored in platelets as an effective gene therapy strategy for both hemophilia A and B
Wang D, Shao X, Wang Q, Pan X, Dai Y, Yao S, Yin T, Wang Z, Zhu J, Xi X, Chen Z, Chen S, Zhang G (2021) Activated factor X targeted stored in platelets as an effective gene therapy strategy for both hemophilia A and B. Clin Transl Med 11(3):e375. doi: 10.1002/ctm2.375
Summary: Treatment of hemophiliacs with inhibitors remains challenging, and new treatments are in urgent need. Coagulation factor X plays a critical role in downstream blood coagulation cascade, which could serve as a bypassing agent for hemophilia therapy. Target expression of the FXa precursor to platelets can generate a storage pool of FXa in platelet α-granules, the platelet-stored FXa is effective in treating HA and HB with inhibitors, suggesting that this could be a novel choice for hemophilia patients with inhibitors.
Usage: A single dose of CD45.2-SAP (biotinylated Anti-CD45 mixed with Streptavidin-ZAP) enabled efficient engraftment of donor cells (> 90%) and full correction of sickle-cell anemia. (3 mg/kg iv; Palchaudhuri et al.).
Related Products: Streptavidin-ZAP (Cat. #IT-27)
P75 and S100 gene expression induced by cell-imprinted substrate and beta-carotene to nerve tissue engineering
Dadashkhan S, Irani S, Bonakdar S, Ghalandari B (2021) P75 and S100 gene expression induced by cell-imprinted substrate and beta-carotene to nerve tissue engineering. Journal of Applied Polymer Science 138(26):50624. doi: 10.1002/app.50624
Objective: Aim to differentiate adipose-derived stem cells (ADSCs) to Schwann cells (SCs), as one of the major cell sources in nerve regeneration.
Summary: Use of synergistic application of imprinting method and beta-carotene BC. Schwann cell imprinted substrates can mimic the morphology and topography of SCs and induce differentiation signals in mesenchymal stem cells. BC stimulates neural differentiation.
Usage: Immunocytochemistry
Related Products: NGFr (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)
Central opioid receptors mediate morphine-induced itch and chronic itch via disinhibition
Wang Z, Jiang C, Yao H, Chen O, Rahman S, Gu Y, Zhao J, Huh Y, Ji RR (2021) Central opioid receptors mediate morphine-induced itch and chronic itch via disinhibition. Brain 144(2):665-681. doi: 10.1093/brain/awaa430
Summary: Itch is a common side effect of opioids, particularly as a result of epidural or intrathecal administration. Notably, morphine-elicited itch was suppressed by intrathecal administration of NPY and abolished by spinal ablation of GRPR+ neurons with intrathecal injection of Bombesin-SAP.
Usage: For ablation of GRPR+ neurons, mice were given an intrathecal injection of 400 ng Bombesin-SAP or Blank-SAP (control) 10 days before behavioral testing.
Related Products: Bombesin-SAP (Cat. #IT-40), Blank-SAP (Cat. #IT-21)
The HIF-1α antisense long non-coding RNA drives a positive feedback loop of HIF-1α mediated transactivation and glycolysis.
Zheng F, Chen J, Zhang X, Wang Z, Chen J, Lin X, Huang H, Fu W, Liang J, Wu W, Li B, Yao H, Hu H, Song E (2021) The HIF-1α antisense long non-coding RNA drives a positive feedback loop of HIF-1α mediated transactivation and glycolysis. Nat Commun 12(1):1341. doi: 10.1038/s41467-021-21535-3 PMID: 33637716
Objective: To investigate the contributions of HIF-1 in regulating glycolysis of cancer cells under hypoxia
Summary: HIF Antisense LncRNA (HIFAL) has a critical regulatory role in HIF-1-driven transactivation and glycolysis, identifying HIFAL as a therapeutic target of cancer treatment.
Usage: Western blot (1:500)
Related Products: Trans-4-Hydroxy-L-Proline Rabbit Polyclonal, Conjugated (Cat. #AB-T044)