Wang YQ, Liu WY, Li L, Qu WM, Huang ZL (2021) Neural circuitry underlying REM sleep: A review of the literature and current concepts. Prog Neurobiol 204:102106. doi: 10.1016/j.pneurobio.2021.102106
Summary: To investigate the role of the LC in sleep the authors injected 0.3 µl of 192-Saporin (Cat. IT-01) or anti-DBH-SAP (Cat. #IT-03) at 1 µg/µl. They also used 0.3 µl of orexin-SAP (Cat. #IT-20) at either 90 ng/µl or 60 ng/µl in a separate group of animals. The results indicate that orexin innervation to the pons plays a role in arousal from sleep.
See: Blanco-Centurion C et al. Effects of hypocretin2-saporin and antidopamine-beta-hydroxylase-saporin neurotoxic lesions of the dorsolateral pons on sleep and muscle tone. Eur J Neurosci 19(10):2741-2752, 2004.
Related Products: Orexin-B-SAP (Cat. #IT-20)