References

Li Q, Syrovets T, Simmet T, Ding J, Xu J, Chen W, Zhu D, Gao P (2013) Plasmin induces intercellular adhesion molecule 1 expression in human endothelial cells via nuclear factor-κB/mitogen-activated protein kinases-dependent pathways. Exp Biol Med (Maywood) 238(2):176-186. doi: 10.1177/1535370212473700

Summary: Intracellular adhesion molecule 1 (ICAM-1) mediates inflammatory cell migration – an early step in atherosclerosis. The authors investigated an inflammatory cascade activated by plasmin using a variety of methods, including flow cytometry with anti-mouse IgG-FITC (Cat. #FL-07) and anti-rabbit IgG-FITC (Cat. #FL-04).

Related Products: Goat Anti-Rabbit IgG, FITC-labeled (Cat. #FL-04), Goat Anti-Mouse IgG, FITC-labeled (Cat. #FL-07)

Zheng H, Rinaman L (2013) Yohimbine anxiogenesis in the elevated plus maze requires hindbrain noradrenergic neurons that target the anterior ventrolateral bed nucleus of the stria terminalis. Eur J Neurosci 37(8):1340-1349. doi: 10.1111/ejn.12123

Summary: The anterior ventrolateral bed nucleus of the stria terminalis (vIBST) appears to be important for increased noradrenergic signaling to trigger anxiety-like behavior. 42.8 ng of anti-DBH-SAP (Cat. #IT-03) was administered to the vIBST of rats in bilateral injections. Elimination of noradrenergic neurons in the vIBST abolished yohimbine-induced anxiogenesis in an elevated plus maze, indicating that hindbrain noradrenergic neurons targeting the vIBST are involved in this mechanism.

Related Products: Anti-DBH-SAP (Cat. #IT-03)

Brayda-Bruno L, Mons N, Yee BK, Micheau J, Abrous DN, Nogues X, Marighetto A (2013) Partial loss in septo-hippocampal cholinergic neurons alters memory-dependent measures of brain connectivity without overt memory deficits. Neurobiol Dis 54:372-381. doi: 10.1016/j.nbd.2013.01.010

Summary: The authors examined whether partial degeneration of septo-hippocampal neurons alters brain activity patterns even without overt memory loss. Mice received 45 ng of mu p75-SAP (Cat. #IT-16) into the medial septal area. Lesioned animals had significantly altered functional activities in the brain, despite lack of an overt behavioral deficit. Some changes observed are also altered with the initial signs of Alzheimer’s disease.

Related Products: mu p75-SAP (Cat. #IT-16)

Torres Demichelis V, Vilcaes AA, Iglesias-Bartolome R, Ruggiero FM, Daniotti JL (2013) Targeted delivery of immunotoxin by antibody to ganglioside GD3: A novel drug delivery route for tumor cells. PLoS One 8(1):e55304. doi: 10.1371/journal.pone.0055304

Summary: The authors used the mouse monoclonal antibody R24 against ganglioside G3 with Mab-ZAP (Cat. #IT-04) to test the viability of ganglioside G3 as a cancer therapy target. Varying concentrations of R24 were used on various cell lines with either 0.95 nM or 9.5 nM Mab-ZAP depending on the cell line.

Related Products: Mab-ZAP (Cat. #IT-04)

Read the featured article in Targeting Trends.

Ren C, Luan L, Wui-Man Lau B, Huang X, Yang J, Zhou Y, Wu X, Gao J, Pickard GE, So KF, Pu M (2013) Direct retino-raphe projection alters serotonergic tone and affective behavior. Neuropsychopharmacology 38(7):1163-1175. doi: 10.1038/npp.2013.35

Summary: Although recent work has shown that some intrinsically photosensitive retinal ganglion cells (ipRGCs) are responsible for processing nonimage-forming visual functions, it is unclear whether the ipRGCs or conventional RGCs modulate affective behavior. The authors injected 2 μg of melanopsin-SAP (Cat. #IT-44) into each eye of gerbils, or biotinylated CTB monoclonal antibody coupled to Streptavidin-ZAP (Cat. #IT-27). The data suggest that retino-raphe signals modulate dorsal raphe nucleus serotonergic tone and affective behavior.

Related Products: Melanopsin-SAP (Cat. #IT-44), Streptavidin-ZAP (Cat. #IT-27)

Szigeti C, Bencsik N, Simonka AJ, Legradi A, Kasa P, Gulya K (2013) Long-term effects of selective immunolesions of cholinergic neurons of the nucleus basalis magnocellularis on the ascending cholinergic pathways in the rat: A model for Alzheimer’s disease. Brain Res Bull 94C:9-16. doi: 10.1016/j.brainresbull.2013.01.007

Summary: 192-IgG-SAP (Cat. #IT-01) has been used extensively to generate models of Alzheimer’s disease in rats. In this work, the authors detailed the time course of neuronal loss with an eye on potential recovery from the lesion. The nucleus basalis magnocellularis of rats was injected with 75 ng of 192-IgG-SAP (Cat. #IT-01) and long-term changes were tracked by immunohistochemistry. While some acetylcholinesterase neurons, considered cholinoceptive, were lost, choline acetyltransferase (cholinergic) neurons sustained a massive irreversible reduction in number.

Related Products: 192-IgG-SAP (Cat. #IT-01)

Shia WW, Bailey RC (2013) Single domain antibodies for the detection of ricin using silicon photonic microring resonator arrays. Anal Chem 85(2):805-810. doi: 10.1021/ac3030416 PMID: 23268548

Summary: A major hurdle to clear in the fight against bioterrorism is the ability to identify various biowarfare agents. One of the more difficult substances to identify is ricin. This work describes the use of single domain antibodies to identify ricin in a microring resonator array assay. Saporin (Cat. #PR-01) along with affinity purified chicken anti-saporin (Cat. #AB-17AP) were used as controls when constructing the assay. The results demonstrate the feasibility of using microring resonator arrays for the detection of biowarfare agents.

Related Products: Saporin Chicken Polyclonal, affinity-purified (Cat. #AB-17AP), Saporin (Cat. #PR-01)

Hamlin AS, Windels F, Boskovic Z, Sah P, Coulson EJ (2013) Lesions of the basal forebrain cholinergic system in mice disrupt idiothetic navigation. PLoS One 8(1):e53472. doi: 10.1371/journal.pone.0053472

Summary: Alzheimer’s disease patients perform poorly on spatial navigation tests requiring either distal cues (allothetic) or body-centered cues (idiothetic). The authors used 0.2 μg bilateral infusions of mu p75-SAP (Cat. #IT-16) into the lateral ventricles of mice to examine the hypothesis that the cholinergic medial septo-hippocampal circuit is important for idiothetic navigation. Rabbit IgG-SAP (Cat. #IT-35) was used as a control. Lesioned animals were similar to controls in contextual fear conditioning, spatial working memory, as well as several other parameters. But exploratory behavior requiring idiothetic signals was very disorganized, indicating that cholinergic cells are vital to idiothetic navigation.

Related Products: mu p75-SAP (Cat. #IT-16), Rabbit IgG-SAP (Cat. #IT-35)

Ferrini F, Trang T, Mattioli TA, Laffray S, Del’Guidice T, Lorenzo LE, Castonguay A, Doyon N, Zhang W, Godin AG, Mohr D, Beggs S, Vandal K, Beaulieu JM, Cahill CM, Salter MW, De Koninck Y (2013) Morphine hyperalgesia gated through microglia-mediated disruption of neuronal Cl(-) homeostasis. Nat Neurosci 16(2):183-192. doi: 10.1038/nn.3295

Summary: Although morphine is the drug of choice in dealing with chronic pain, it paradoxically can produce a hyperalgesic state. The authors examined the issue from several different angles. One method was to eliminate spinal microglia of rats through the intrathecal application of 16-32 μg of Mac-1-SAP (Cat. #IT-33). 20 μg of saporin (Cat. #PR-01) was used as a control. It was found that P2X4 receptors expressed by microglia were necessary for the development of morphine hyperalgesia, but not morphine tolerance.

Related Products: Mac-1-SAP rat (Cat. #IT-33), Saporin (Cat. #PR-01)

Read the featured article in Targeting Trends.

Menendez L, Kulik MJ, Page AT, Park SS, Lauderdale JD, Cunningham ML, Dalton S (2013) Directed differentiation of human pluripotent cells to neural crest stem cells. Nat Protoc 8(1):203-212. doi: 10.1038/nprot.2012.156 PMID: 23288320

Summary: This protocol describes in detail how to perform a highly efficient, lineage-specific differentiation of human pluripotent cells to a NCSC fate.

Usage: 0.2 ul per 10^6 cells

Related Products: NGFr (ME20.4, p75) Mouse Monoclonal (Cat. #AB-N07)