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Selective lesion of GABA-ergic neurons in the medial septum by GAT1-saporin impairs spatial learning in a water-maze.
Burjanadze M, Mataradze S, Rusadze K, Chkhikvishvili N, Dashniani M (2015) Selective lesion of GABA-ergic neurons in the medial septum by GAT1-saporin impairs spatial learning in a water-maze. Georgian Med News 240:59-64.
Summary: The authors investigated the role of GABAergic neurons in the medial septum on spatial learning using a Morris water maze test. Rats received bilateral injections totaling 162 ng of GAT-1-SAP (Cat. #IT-32) into the medial septum. Saporin (Cat. #PR-01) was used as a control. The lesioned animals displayed significant deficits during the water maze task, indicating that GABAergic neurons in the medial septum are intrinsic to organization of spatial map-driven behavior.
Related Products: GAT1-SAP (Cat. #IT-32), Saporin (Cat. #PR-01)
Characteristic patterns of dendritic remodeling in early-stage glaucoma: evidence from genetically identified retinal ganglion cell types.
El-Danaf R, Huberman A (2015) Characteristic patterns of dendritic remodeling in early-stage glaucoma: evidence from genetically identified retinal ganglion cell types. J Neurosci 35:2329-2343. doi: 10.1523/JNEUROSCI.1419-14.2015 PMID: 25673829
Summary: The loss of retinal ganglion cells (RGC) is the second-most common cause of blindness worldwide. Using several mouse transgenic cell lines, the authors investigated the changes that occur on the establishment of elevated ocular pressure. Anti-melanopsin (Cat. #AB-N39) at 1:1000 was used to illuminate the morphology of the M1 intrinsically photosensitive RGC.
Related Products: Melanopsin Rabbit Polyclonal, affinity-purified (Cat. #AB-N39)
Activation of the mouse primary visual cortex by medial prefrontal subregion stimulation is not mediated by cholinergic basalo-cortical projections.
Nguyen H, Huppé-Gourgues F, Vaucher E (2015) Activation of the mouse primary visual cortex by medial prefrontal subregion stimulation is not mediated by cholinergic basalo-cortical projections. Front Syst Neurosci 9:1. doi: 10.3389/fnsys.2015.00001
Summary: Mice received 1 μg icv injections of mu p75-SAP (Cat. #IT-16) to eliminate NGFr-positive cells. The results indicate a link between the prelimbic and infralimbic cortices and the primary visual cortex.
Related Products: mu p75-SAP (Cat. #IT-16)
Human embryonic stem cells form functional thyroid follicles
Ma R, Latif R, Davies TF (2015) Human embryonic stem cells form functional thyroid follicles. Thyroid 25(4):455-461. doi: 10.1089/thy.2014.0537 PMID: 25585054
Summary: Lysates were prepared from single- and double-transfected cells by treating with 1 ·RIPA buffer for 20 min on ice. The samples were then centrifuged and treated with 5 · sample buffer at 100°C for 5 min and loaded on a 4–15% SDS-PAGE gel. Western blotting was performed according to a standard protocol.
Related Products: TSH Receptor (A10) Mouse Monoclonal (Cat. #AB-N16)
Triterpenoid saponin augmention of saporin-based immunotoxin cytotoxicity for human leukaemia and lymphoma cells is partially immunospecific and target molecule dependent
Holmes SE, Bachran C, Fuchs H, Weng A, Melzig MF, Flavell SU, Flavell DJ. (2015) Triterpenoid saponin augmention of saporin-based immunotoxin cytotoxicity for human leukaemia and lymphoma cells is partially immunospecific and target molecule dependent. Immunopharmacol Immunotoxicol 37(1):42-55. doi: 10.3109/08923973.2014.971964 PMID: 25347443
Effects of immunotoxic and electrolytic lesions of medial septal area on spatial short-term memory in rats.
Dashniani M, Kruashvili L, Rusadze K, Matatradze S, Beselia G (2015) Effects of immunotoxic and electrolytic lesions of medial septal area on spatial short-term memory in rats. Georgian Med News 239:98-103.
Summary: In this work the authors investigated how essential septohippocampal projections are in a spatial working memory model. Rats received bilateral injections of 192-IgG-SAP (Cat. #IT-01, 600 ng total) or GAT-1-SAP (Cat. #IT-32, 195 ng total) into the medial septum. Saporin (Cat. #PR-01) was used as a control.
Related Products: 192-IgG-SAP (Cat. #IT-01), GAT1-SAP (Cat. #IT-32), Saporin (Cat. #PR-01)
Mesenchymal stem cells recruit macrophages to alleviate experimental colitis through TGFβ1
Liu W, Zhang S, Gu S, Sang L, Dai C (2015) Mesenchymal stem cells recruit macrophages to alleviate experimental colitis through TGFβ1. Cell Physiol Biochem 35:858-865. doi: 10.1159/000369743
Usage: For in vivo depletion of macrophages, mice received i.v. injection of Mac-1-SAP 20 µg, twice per week.
Related Products: Mac-1-SAP mouse/human (Cat. #IT-06)
Distinct behavioral consequences of short-term and prolonged GABAergic depletion in prefrontal cortex and dorsal hippocampus
Reichel JM, Nissel S, Rogel-Salazar G, Mederer A, Käfer K, Bedenk BT, Martens H, Anders R, Grosche J, Michalski D, Härtig W, Wotjak CT (2015) Distinct behavioral consequences of short-term and prolonged GABAergic depletion in prefrontal cortex and dorsal hippocampus. Front Behav Neurosci 8:452. doi: 10.3389/fnbeh.2014.00452 PMID: 25628548
Objective: To study the effects of GABAergic interneurons on exploratory and cognitive behavior and their involvement in schizophrenia-related symptoms.
Summary: The authors demonstrated that GABAergic lesions result in distinct behavioral phenotypes specific to the target site and modulated by incubation time. They were able to simulate a pathological disease state and the consequences of prolonged GABAergic depletion.
Related Products: Anti-vGAT-SAP (Cat. #IT-71)
See Also:
Dual targeting NG2 and GD3A using Mab-Zap immunotoxin results in reduced glioma cell viability in vitro.
Higgins S, Fillmore H, Ashkan K, Butt A, Pilkington G (2015) Dual targeting NG2 and GD3A using Mab-Zap immunotoxin results in reduced glioma cell viability in vitro. Anticancer Res 35:77-84.
Summary: Human glioma-derived cell lines were sequentially incubated with anti-NG2 and anti-GD3A coupled to Mab-ZAP (Cat. #IT-04) at 1 μg/ml and 5 μg/ml for 72 hours each. The combination therapy was significantly more effective than single therapy in eliminating the glioma cells.
Related Products: Mab-ZAP (Cat. #IT-04)
Targeted ablation of cholinergic interneurons in the dorsolateral striatum produces behavioral manifestations of Tourette syndrome.
Xu M, Kobets A, Du J, Lennington J, Li L, Banasr M, Duman R, Vaccarino F, DiLeone R, Pittenger C (2015) Targeted ablation of cholinergic interneurons in the dorsolateral striatum produces behavioral manifestations of Tourette syndrome. Proc Natl Acad Sci U S A 112:893-898. doi: 10.1073/pnas.1419533112
Summary: Postmortem studies of Tourette syndrome patients has revealed a reduction in the number of specific striatal interneurons. The authors explored the hypothesis that this neuronal deficit is enough to produce the symptoms of Tourette syndrome in mice. Animals received 90-ng injections of Anti-ChAT-SAP (Cat. #IT-42) into the striatum. Rabbit IgG-SAP (Cat. #IT-35) was used as a control. The data suggest that loss of the striatal interneurons is enough to produce some, but not all, of the symptoms caused by Tourette syndrome.
Related Products: Anti-ChAT-SAP (Cat. #IT-42), Rabbit IgG-SAP (Cat. #IT-35)