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Basic fibroblast growth factor (FGF-2) is a member of the fibroblast growth factor family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration […]

Leptin is a 16-kDa peptide hormone secreted from white adipocytes and implicated in the regulation of food intake and energy balance. It provides the key afferent signal from fat cells in the feedback system that controls body fat stores. Leptin Human Recombinant, Cat. #PRP-228CYTavailable in these sizes: 200 micrograms, 1 milligram, and 5 milligrams Related

Granulocyte Macrophage Colony Stimulating Factor (GM CSF) is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with

Growth Hormone (GH) is a member of the somatotropin/prolactin family of hormones which play an important role in growth control. The gene, along with four other related genes, is located at the growth hormone locus on chromosome 17 where they are interspersed in the same transcriptional orientation; an arrangement which is thought to have evolved

Anti-dopamine beta-hydroxylase-saporin (anti-DBH-SAP, Cat. #IT-03) is a highly specific noradrenergic lesioning agent. It specifically targets rat cells that express dopamine beta-hydroxylase and is also reported to react with mouse protein.1 This vesicular enzyme is exposed to the exterior milieu upon release of noradrenaline and thus allows these cells to be targeted with saporin. The specificity

GAT-1 is a sodium-coupled neurotransmitter transporter responsible for moving g-aminobutyric acid (GABA) across cell membranes. GABA is the predominant inhibitory neurotransmitter in the mammalian central nervous system. GAT-1 is widely distributed in both the central and peripheral nervous systems. GAT-1 and GABA are present in numerous neuronal pathways, some of which are implicated in epilepsy,

Targeting in mouse: mu p75-SAP (Cat. #IT-16) To create this targeted toxin, we affinity-purified the rabbit polyclonal with the immunogen bound to a solid support, and conjugated the affinity-purified antibody (Cat. #AB-N01AP) to saporin. As can be seen in the cytotoxicity assay below, mu p75-SAP has an EC50 in the picomolar range. This greater potency

CTB-SAP (Cat. #IT-14) is a conjugate between the cell-binding component of cholera toxin (the B chain) and saporin. CTB binds to GM1 (monosialotetrahexosylganglioside), which is present on the surface of different neurons. It has been suggested to be involved in many problems (besides the most famous in the gut: cholera) of neuronal systems: Parkinson’s, motor

New Secondary Conjugates – Fab-ZAPs – Use Monovalent Secondary Antibodies Linked to Saporin For the past decade scientists have extensively used secondary conjugates (e.g. Mab-ZAP; Cat. #IT-04, and Hum-ZAP; Cat. #IT-22) to make their own targeted toxins for in vitro use. The ability to combine: 1) a primary antibody to a cell-surface marker with 2)

Anti-6 His-SAP (Cat. #IT-52) Composed of an antibody to 6 His conjugated to saporin, this secondary conjugate should be used as a diagnostic tool for testing your primary, 6 His-tagged proteins for specific cell surface epitope binding and internalization. The 6 His tag is widely used because of its affinity to bind nickel or cobalt