Q: How do I know my antibody will work in an immunotoxin? A: We recommend that you use one of our second immunotoxins (secondary conjugates) to test for specificity and internalization of your antibody. Q: How much antibody do I need to provide for a custom conjugation? A: The required quantity is 8-15 mg of […]
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Cassel JC (2002) Featured Article: Does 192-IgG-Saporin or 5,7-DHT kill cognitive functions in the rat?. Targeting Trends 3(4) Related Products: 192-IgG-SAP (Cat. #IT-01) Read the featured article in Targeting Trends. See Also: Lehmann O et al. 5,7-DHT-induced hippocampal 5-HT depletion attenuates behavioural deficits produced by 192 IgG-saporin lesions of septal cholinergic neurons in the rat.
Featured Article: Does 192-IgG-Saporin or 5,7-DHT kill cognitive functions in the rat? Read More »
Zeitschel U, Schliebs R, Rossner S, Bigl V, Eschrich K, Bigl M (2002) Changes in activity and expression of phosphofructokinase in different rat brain regions after basal forebrain cholinergic lesion. J Neurochem 83(2):371-380. doi: 10.1046/j.1471-4159.2002.01127.x Summary: The authors used intraventricular injections of 4 µg of 192-Saporin (Cat. #IT-01) in rats to investigate whether impaired cholinergic
Contributed by Jean-Christophe Cassel, PhD, Laboratoire de Neurosciences Cognitives et Comportementales, UMR 7521 Université Louis Pasteur/CNRS, Strasbourg, France & Institut für Experimentelle und Klinische Pharmakologie und Toxikologie der Universität Freiburg, Neuropharmakologisches Labor, Freiburg, Germany. Dr. Cassel and colleagues are the authors of four publications released this quarter using 192-Saporin (Cat. #IT-01) Trying to understand how
Cover Article: Does 192-IgG-Saporin or 5,7-DHT kill cognitive functions in the rat? Read More »
Ballmaier M, Casamenti F, Scali C, Mazzoncini R, Zoli M, Pepeu G, Spano PF (2002) Rivastigmine antagonizes deficits in prepulse inhibition induced by selective immunolesioning of cholinergic neurons in nucleus basalis magnocellularis. Neuroscience 114(1):91-98. doi: 10.1016/s0306-4522(02)00234-8 Summary: The authors injected 300 nl of 400 ng/ml 192-Saporin (Cat. #IT-01) bilaterally into the nucleus basalis magnocellularis of
Cassel J, Gaurivaud M, Lazarus C, Bertrand F, Galani R, Jeltsch H (2002) Grafts of fetal septal cells after cholinergic immunotoxic denervation of the hippocampus: a functional dissociation between dorsal and ventral implantation sites. Neuroscience 113(4):871-882. doi: 10.1016/s0306-4522(02)00226-9 Summary: The authors lesioned rats with intraseptal infusions of 0.8 µg 192-Saporin (Cat. #IT-01), then implanted fetal
Vale-Martinez A, Baxter MG, Eichenbaum H (2002) Selective lesions of basal forebrain cholinergic neurons produce anterograde and retrograde deficits in a social transmission of food preference task in rats. Eur J Neurosci 16(6):983-998. doi: 10.1046/j.1460-9568.2002.02153.x Summary: Injections of 0.2 µg 192-Saporin (Cat. #IT-01) were made into either the medial septum/vertical limb of the diagonal band
Pizzo DP, Thal LJ, Winkler J (2002) Mnemonic deficits in animals depend upon the degree of cholinergic deficit and task complexity. Exp Neurol 177:292-305. doi: 10.1006/exnr.2002.7993 Summary: In this study, the authors compared icv and intraparenchymal injections of 192-Saporin (Cat. #IT-01, 3.3 µg and 450 ng, respectively). While a similar reduction in choline acetyltransferase activity
Nattie EE, Li A (2002) Substance P-saporin lesion of neurons with NK1 receptors in one chemoreceptor site in rats decreases ventilation and chemosensitivity. J Physiol 544(Pt 2):603-616. doi: 10.1113/jphysiol.2002.020032 Summary: The authors injected 0.1 pmol SP-SAP (Cat. #IT-07) into the retrotrapezoid nucleus/parapyramidal region of rats. The lesioned animals demonstrated hypoventilation while at rest, decreased response
Truitt WA, Coolen LM (2002) Identification of a potential ejaculation generator in the spinal cord. Science 297(5586):1566-1569. doi: 10.1126/science.1073885 Summary: The authors lesioned a specific population of rat spinothalamic neurons using 6-8 injections of 4 ng SSP-SAP (Cat. #IT-11). Whereas the treated rats exhibited no change in sexual behavior such as mounts and intromissions, ejaculatory
Identification of a potential ejaculation generator in the spinal cord. Read More »