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Custom Antibody-Saporin Conjugates

Q: How do I know my antibody will work in an immunotoxin?

A: We recommend that you use one of our second immunotoxins (secondary conjugates) to test for specificity and internalization of your antibody.

Q: How much antibody do I need to provide for a custom conjugation?

A: The required quantity is 8-15 mg of purified monoclonal antibody.

Q: How much of the saporin conjugate will that give me?

A: The yield is 15-30% in mg of immunotoxin from the number of mg of original antibody.

Q: What is the ratio of saporin to antibody?

A: The amount of saporin can vary between 1.5-3 moles saporin per mole of antibody.

Q: What quality control is involved?  Will you provide product specifications such as average saporin to antibody ratio?

A: We monitor the reactions and purification by several means.  The product is confirmed by gel electrophoresis. A data sheet will be provided when we ship the immunotoxin to inform you of final average molecular weight.

Q: What is the cost of a custom immunotoxin preparation? How long will it take to complete?

A: The standard cost of an antibody-saporin conjugation is US$4500.00 (as of Dec 2007).  From the time we receive the purified antibody to the time we ship out the finished conjugate is 2-3 weeks.

See: Custom Conjugates , ZAP Conjugates

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