Q: How do I know my antibody will work in an immunotoxin?
A: We recommend that you use one of our second immunotoxins (secondary conjugates) to test for specificity and internalization of your antibody.
Q: How much antibody do I need to provide for a custom conjugation?
A: The required quantity is 8-15 mg of purified monoclonal antibody.
Q: How much of the saporin conjugate will that give me?
A: The yield is 15-30% in mg of immunotoxin from the number of mg of original antibody.
Q: What is the ratio of saporin to antibody?
A: The amount of saporin can vary between 1.5-3 moles saporin per mole of antibody.
Q: What quality control is involved? Will you provide product specifications such as average saporin to antibody ratio?
A: We monitor the reactions and purification by several means. The product is confirmed by gel electrophoresis. A data sheet will be provided when we ship the immunotoxin to inform you of final average molecular weight.
Q: What is the cost of a custom immunotoxin preparation? How long will it take to complete?
A: The standard cost of an antibody-saporin conjugation is US$4500.00 (as of Dec 2007). From the time we receive the purified antibody to the time we ship out the finished conjugate is 2-3 weeks.
See: Custom Conjugates , ZAP Conjugates