Kristen Hartman – Page 202 – Advanced Targeting Systems

Participation of hindbrain AMP-activated protein kinase in glucoprivic feeding.

Li AJ, Wang Q, Ritter S (2011) Participation of hindbrain AMP-activated protein kinase in glucoprivic feeding. Diabetes 60(2):436-442. doi: 10.2337/db10-0352 Summary: Catecholamine neurons innervating the medial hypothalamus are involved in the control of glucoprivic feeding as well as other responses to glucose deficit. Rats received bilateral 82-ng injections of anti-DBH-SAP (Cat. #IT-03) into the paraventricular […]

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Donepezil plus estradiol treatment enhances learning and delay-dependent memory performance by young ovariectomized rats with partial loss of septal cholinergic neurons.

Kristen Hartman

Gibbs RB, Chipman AM, Nelson D (2011) Donepezil plus estradiol treatment enhances learning and delay-dependent memory performance by young ovariectomized rats with partial loss of septal cholinergic neurons. Horm Behav 59(4):503-511. doi: 10.1016/j.yhbeh.2011.01.011 Summary: Among the beneficial effects of estrogen on the brain are improved cognitive performance and prevention of age-related cognitive decline. These positive

Donepezil plus estradiol treatment enhances learning and delay-dependent memory performance by young ovariectomized rats with partial loss of septal cholinergic neurons. Read More »

Lesion of cholinergic neurons in nucleus basalis enhances response to general anesthetics.

Kristen Hartman

Leung LS, Petropoulos S, Shen B, Luo T, Herrick I, Rajakumar N, Ma J (2011) Lesion of cholinergic neurons in nucleus basalis enhances response to general anesthetics. Exp Neurol 228(2):259-269. doi: 10.1016/j.expneurol.2011.01.019 Summary: Consciousness and response to general anesthesia have been linked to acetylcholine in the brain. The authors treated rats with 150-ng bilateral injections

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PreBotzinger complex neurokinin-1 receptor-expressing neurons mediate opioid-induced respiratory depression.

Kristen Hartman

Montandon G, Qin W, Liu H, Ren J, Greer JJ, Horner RL (2011) PreBotzinger complex neurokinin-1 receptor-expressing neurons mediate opioid-induced respiratory depression. J Neurosci 31(4):1292-1301. doi: 10.1523/JNEUROSCI.4611-10.2011 PMID: 21273414 Summary: In order to identify the neurons involved with respiratory depression due to administration of opioids, some neuro- transmission networks in the preBotzinger complex were locally

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Generation of human melanocytes from induced pluripotent stem cells

Kristen Hartman

Ohta S, Imaizumi Y, Okada Y, Akamatsu W, Kuwahara R, Ohyama M, Amagai M, Matsuzaki Y, Yamanaka S, Okano H, Kawakami Y (2011) Generation of human melanocytes from induced pluripotent stem cells. PLoS One 6(1):e16182. doi: 10.1371/journal.pone.0016182 PMID: 21249204 Summary: For immunocytochemistry, cells were fixed with PBS containing 4% PFA for 20 min at room

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Targeting Tools: Anti-DBH-SAP

News Tools / Kristen Hartman

Anti-dopamine beta-hydroxylase-saporin (anti-DBH-SAP, Cat. #IT-03) is a highly specific noradrenergic lesioning agent. It specifically targets rat cells that express dopamine beta-hydroxylase and is also reported to react with mouse protein.1 This vesicular enzyme is exposed to the exterior milieu upon release of noradrenaline and thus allows these cells to be targeted with saporin. The specificity

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Destroy Hippocampal Neurons

Kristen Hartman

Q: Is there a saporin derivative available that selectively destroys all or some hippocampal neurons? A: Assuming that you mean to eliminate cell bodies from the hippocampus, rather than just projections, a neat paper by Martin et al. describes the use of SSP-SAP (Cat #IT-11) to do this. NPY-SAP (Cat. #IT-28) could also be interesting.

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Custom Mouse IgM Conjugate and Controls

Kristen Hartman

Q: When we contacted you to find out more about having a custom saporin conjugation performed with our primary antibody, you recommended that we use the ATS secondary conjugate system to determine that our antibody was specific to the population we want to eliminate. We looked more at the website, and it seems that we

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Time Course for Toxins

Kristen Hartman

Q: I am interested in your product GAT1-SAP (Cat. #IT-32). Before ordering, I would like to know how long it takes until the toxin produces a lesion; Is seven days enough? A: The usual time-course for saporin toxins is that behavioral effects start appearing at four days and usually plateau at seven days. However, keep

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Corticotropin-releasing factor critical for zebrafish camouflage behavior is regulated by light and sensitive to ethanol.

Kristen Hartman

Wagle M, Mathur P, Guo S (2011) Corticotropin-releasing factor critical for zebrafish camouflage behavior is regulated by light and sensitive to ethanol. J Neurosci 31(1):214-224. doi: 10.1523/JNEUROSCI.3339-10.2011 PMID: 21209207 Objective: To explore neural circuit assembly and function using the hardwired camouflage response of zebrafish. Summary: Both light exposure and ethanol affect the camouflage response. Understanding

Corticotropin-releasing factor critical for zebrafish camouflage behavior is regulated by light and sensitive to ethanol. Read More »

Author name: Kristen Hartman