Author name: Kristen Hartman

Sudden death and myocardial lesions after damage to catecholamine neurons of the nucleus tractus solitarii in rat.

Talman WT, Dragon DN, Jones SY, Moore SA, Lin LH (2012) Sudden death and myocardial lesions after damage to catecholamine neurons of the nucleus tractus solitarii in rat. Cell Mol Neurobiol 32(7):1119-1126. doi: 10.1007/s10571-012-9835-1 Summary: Previous work has shown that elimination of neurons expressing the neurokinin-1 receptor (NK1r) from the nucleus tractus solitarii (NTS) causes […]

Sudden death and myocardial lesions after damage to catecholamine neurons of the nucleus tractus solitarii in rat. Read More »

Targeting Tools: Anti-RFP

Red fluorescent protein (RFP) is derived from Discosoma sea anemone. This antibody (Anti-RFP, Cat. #AB-332) recognizes native and denatured forms of RFP. The antigen for this antibody is present in all of these fluorescent molecules: RFP, tag-RFP, turbo-RFP, DeRed, mCherry, and mOrange. The epitope for the antibody is part of the RFP N-terminal sequence. RFP

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Targeting Tools: NK3-SAP

An analog of the Neurokinin B peptide, [MePhe7]-NKB, is a selective agonist of the NK3 receptor. Conjugation of [MePhe7]-NKB to saporin (NK3-SAP) allows for the specific elimination of a subpopulation of neurons expressing kisspeptin, neurokinin B, and dynorphin (KNDy neurons) through their co-expression of the NK3 receptor. Although NK3 receptor expressing neurons reside in several

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Targeting Tools: Chick-ZAP

Chick-ZAP is the most recent in our line of Secondary Conjugates. It is a chemical conjugate of affinity-purified rabbit anti-chicken IgY and the ribosome-inactivating protein, saporin. The conjugate will target and eliminate cells that recognize your primary antibody. This reagent can be utilized for screening chicken IgY antibodies for internalization and/or their suitability to make

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Reducing Agent in Media

Q:  We will be using your chick-ZAP secondary conjugate (Cat. #IT-62) and noticed that in your protocol you mention not to use a reducing agent in your media. Our normal growth media contains beta mercaptoethanol at 100 μM. Will this be a problem? A: Officially, we would recommend allowing the cells to acclimate to media

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Analgesia targeting IB4-positive neurons in cancer-induced mechanical hypersensitivity.

Ye Y, Dang D, Viet CT, Dolan JC, Schmidt BL (2012) Analgesia targeting IB4-positive neurons in cancer-induced mechanical hypersensitivity. J Pain 13(6):524-531. doi: 10.1016/j.jpain.2012.01.006 Summary: DOR (δ opioid receptor) agonists produce minimal side effects and do not lead to tolerance, making them potential alternatives to the widely used μ opioid receptor agonists. Utilizing the fact

Analgesia targeting IB4-positive neurons in cancer-induced mechanical hypersensitivity. Read More »

Featured Article: Use of a novel saporin conjugate (NK3-SAP) to study the function of neurokinin 3 receptor (NK3r)-expressing kisspeptin/neurokinin B/dynorphin (KNDy) neurons in the rat arcuate nucleus

Rance NE, Mittelman-Smith MA, Krajewski-Hall SJ (2012) Featured Article: Use of a novel saporin conjugate (NK3-SAP) to study the function of neurokinin 3 receptor (NK3r)-expressing kisspeptin/neurokinin B/dynorphin (KNDy) neurons in the rat arcuate nucleus. Targeting Trends 13(2) Related Products: NKB-SAP (Cat. #IT-63), Blank-SAP (Cat. #IT-21) Read the featured article in Targeting Trends. See Also: Mittelman-Smith

Featured Article: Use of a novel saporin conjugate (NK3-SAP) to study the function of neurokinin 3 receptor (NK3r)-expressing kisspeptin/neurokinin B/dynorphin (KNDy) neurons in the rat arcuate nucleus Read More »

Neonatal cholinergic lesion alters the acoustic structure of infant rat vocalization but not the early cognitive development.

Kruger HS, Hanganu-Opatz IL (2013) Neonatal cholinergic lesion alters the acoustic structure of infant rat vocalization but not the early cognitive development. Dev Psychobiol 55(3):294-308. doi: 10.1002/dev.21029 Summary: The architecture of the cerebral cortex is dependent on cholinergic innervations for proper maturation and network assembly. The authors administered 0.1 μg of 192-IgG-SAP (Cat. #IT-01) to

Neonatal cholinergic lesion alters the acoustic structure of infant rat vocalization but not the early cognitive development. Read More »

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