Q: We will be using your chick-ZAP secondary conjugate (Cat. #IT-62) and noticed that in your protocol you mention not to use a reducing agent in your media. Our normal growth media contains beta mercaptoethanol at 100 μM. Will this be a problem?
A: Officially, we would recommend allowing the cells to acclimate to media that contains NO BMe, and then proceed with your experiments. However, some of our in-house experiments use cells that are cultured in media containing 50 μM BMe, and we have not seen that concentration affect the toxin’s effectiveness, but we have not tried a concentration as high as 100 μM.
Related Product: Chick-ZAP (Cat. #IT-62)