How can I target B-cells? Which secondary conjugate should I use? ATS makes secondary conjugates that use your primary antibody to target cells for elimination. Just mix your primary antibody with a secondary antibody conjugated to the ribosome-inactivating protein, Saporin, to screen your antibodies. The cells targeted by your primary antibody are eliminated. Recognizes whole […]
Ramos-Rodriguez JJ, Pacheco-Herrero M, Thyssen D, Murillo-Carretero MI, Berrocoso E, Spires-Jones TL, Bacskai BJ, Garcia-Alloza M (2013) Rapid beta-amyloid deposition and cognitive impairment after cholinergic denervation in app/ps1 mice. J Neuropathol Exp Neurol 72(4):272-285. doi: 10.1097/NEN.0b013e318288a8dd Summary: The authors investigated whether specific cholinergic neurodegeneration is responsible for the deposition of plaques. APPswe/PS1dE9 transgenic mice received
Talman WT, Lin LH (2013) Sudden death following selective neuronal lesions in the rat nucleus tractus solitarii. Auton Neurosci 175(1-2):9-16. doi: 10.1016/j.autneu.2012.11.008 Summary: The nucleus tracts solitarii (NTS) is the terminus of cardiovascular reflex nerves. Early work in this area sought to identify transmitters involved in the control of this region. The authors used SSP-SAP
Sudden death following selective neuronal lesions in the rat nucleus tractus solitarii. Read More »
Daniotti JL (2013) Featured Article: Antibodies to glycosphingolipids: An attractive tool for targeted delivery of cytotoxic agents to tumor cells. Targeting Trends 14(2) Related Products: Mab-ZAP (Cat. #IT-04) Read the featured article in Targeting Trends. See Also: Torres Demichelis V et al. Targeted Delivery of Immunotoxin by Antibody to Ganglioside GD3: A Novel Drug Delivery
Contributed by Jose Luis Daniotti, Centro de Investigaciones en Química Biológica de Córdoba (CIQUIBIC, UNC-CONICET), Departamento de Química Biológica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Córdoba, Argentina. Glycosphingolipids (GSLs) are amphipathic molecules consisting of a ceramide lipid moiety linked to a glycan chain of variable length and structure. Among these are found the
Matchynski JJ, Lowrance SA, Pappas C, Rossignol J, Puckett N, Sandstrom M, Dunbar GL (2013) Combinatorial treatment of tart cherry extract and essential fatty acids reduces cognitive impairments and inflammation in the mu-p75 saporin-induced mouse model of Alzheimer’s disease. J Med Food 16(4):288-295. doi: 10.1089/jmf.2012.0131 PMID: 23566055 Summary: The authors investigated the efficacy of a
Minces VH, Alexander AS, Datlow M, Alfonso SI, Chiba AA (2013) The role of visual cortex acetylcholine in learning to discriminate temporally modulated visual stimuli. Front Behav Neurosci 7:16. doi: 10.3389/fnbeh.2013.00016 Summary: In order to examine some of the minor differences in the temporal structure of stimuli, the authors bilaterally injected 37.5 ng of 192-IgG-SAP
Premer C, Lamondin C, Mitzey A, Speth R, Brownfield M (2013) Immunohistochemical localization of AT1a, AT1b, and AT2 Angiotensin II receptor subtypes in the rat adrenal, pituitary, and brain with a perspective commentary. Int J Hypertens 2013:175428. doi: 10.1155/2013/175428 PMID: 23573410 Objective: To immunohistochemically differentiate the angiotensin receptor subtypes: AT-1ARa, AT-1ARb, and AT-2R. Summary: Antibodies
Hernandez L, Flenker K, Hernandez F, Klingelhutz A, McNamara J, Giangrande P (2013) Methods for evaluating cell-specific, cell-internalizing RNA aptamers. Pharmaceuticals (Basel) 6:295-319. doi: 10.3390/ph6030295 Objective: Isolate aptamers that internalize upon binding to their cognate receptor on the cell surface Summary: Among the methods used to characterize aptamers that internalize is a way to monitor
Methods for evaluating cell-specific, cell-internalizing RNA aptamers. Read More »
Laursen B, Mork A, Plath N, Kristiansen U, Bastlund JF (2013) Cholinergic degeneration is associated with increased plaque deposition and cognitive impairment in APPswe/PS1dE9 mice. Behav Brain Res 240:146-152. doi: 10.1016/j.bbr.2012.11.012 Summary: Extracellular plaques containing amyloid β-peptides (Aβ) and cholinergic dysfunction are two of the main hallmarks of Alzheimer’s disease. Using a transgenic mouse line