Ye Y, Bae S, Viet CT, Troob S, Bernabe D, Schmidt BL (2014) IB4(+) and TRPV1(+) sensory neurons mediate pain but not proliferation in a mouse model of squamous cell carcinoma. Behav Brain Funct 10(1):5. doi: 10.1186/1744-9081-10-5 Objective: To evaluate subtypes of sensory neurons involved in cancer pain and proliferation. Summary: IB4(+) neurons play an […]
Ljubojevic V, Luu P, De Rosa E (2014) Cholinergic contributions to supramodal attentional processes in rats. J Neurosci 34(6):2264-2275. doi: 10.1523/JNEUROSCI.1024-13.2014 Summary: Previous work has shown that cholinergic transmission is important for attentional processing of visual stimuli. It is not known whether the role of the cholinergic system is specifically in visual processing, or if
Cholinergic contributions to supramodal attentional processes in rats. Read More »
Hartig W, Saul A, Kacza J, Grosche J, Goldhammer S, Michalski D, Wirths O (2014) Immunolesion-induced loss of cholinergic projection neurones promotes beta-amyloidosis and tau hyperphosphorylation in the hippocampus of triple-transgenic mice. Neuropathol Appl Neurobiol 40(2):106-120. doi: 10.1111/nan.12050 Summary: 3xTg transgenic mice were treated with 2 μg of mu p75-SAP (Cat. #IT-16) into the right
Tai SK, Ma J, Leung LS (2014) Medial septal cholinergic neurons modulate isoflurane anesthesia. Anesthesiology 120(2):392-402. doi: 10.1097/ALN.0b013e3182a7cab6 Summary: General anesthesia is associated with a decrease in cholinergic function. This work examines the effect of volatile anesthetics such as isoflurane or ketamine in the context of cholinergic depletion. Rats received 105-ng bilateral injections of 192-IgG-SAP
Medial septal cholinergic neurons modulate isoflurane anesthesia. Read More »
Suto T, Severino AL, Eisenach JC, Hayashida KI (2014) Gabapentin increases extracellular glutamatergic level in the locus coeruleus via astroglial glutamate transporter-dependent mechanisms. Neuropharmacology 81C:95-100. doi: 10.1016/j.neuropharm.2014.01.040 Summary: Gabapentin is effective in reducing acute and chronic pain, but the mechanisms by which it works are not well understood. The authors assessed extracellular glutamate levels and
Laursen B, Mork A, Plath N, Kristiansen U, Frank Bastlund J (2014) Impaired hippocampal acetylcholine release parallels spatial memory deficits in Tg2576 mice subjected to basal forebrain cholinergic degeneration. Brain Res 1543:253-262. doi: 10.1016/j.brainres.2013.10.055 Summary: The Tg2576 mouse strain provides a limited model for Alzheimer’s disease because they do not display degeneration of cholinergic neurons
Lee LC, Rajkumar R, Dawe GS (2014) Selective lesioning of nucleus incertus with corticotropin releasing factor-saporin conjugate. Brain Res 1543:179-190. doi: 10.1016/j.brainres.2013.11.021 PMID: 24287211 Objective: To investigate the role of Nucleus Incertus (NI) neurons in behavior by lesioning these neurons in rats using CRF-SAP (IT-13). Summary: NI neurons are integral to CRF1, Relaxin-3, and GABA
Roland JJ, Stewart AL, Janke KL, Gielow MR, Kostek JA, Savage LM, Servatius RJ, Pang KC (2014) Medial septum-diagonal band of Broca (MSDB) GABAergic regulation of hippocampal acetylcholine efflux is dependent on cognitive demands. J Neurosci 34(2):506-514. doi: 10.1523/JNEUROSCI.2352-13.2014 Summary: GABAergic and cholinergic neurons in the medial septum-diagonal band of Broca (MSDB) are both involved
Bombesin-SAP (Cat. #IT-40) Bombesin is a 14-amino-acid peptide found in frog-skin. The human equivalent, gastrin releasing peptide (GRP) has been detected in itch pathways and plays a role in eating behaviors. GRP regulates numerous functions of the gastrointestinal and central nervous systems, including release of gastrointestinal hormones, smooth muscle cell contraction, and epithelial cell proliferation.
Targeting Tools: Investigating the Itch Pathway Read More »
Q: How long does it take to see the cell death occurring from the use of targeted toxins using saporin? Is there a time course of hours or days? A: The figure below illustrates the time course of cell death very effectively. Internalization and cytotoxicity of SP-SAP in primary cultures of neonatal spinal cord neurons.
Time Course of Cell Death Read More »