Streptavidin-ZAP [IT-27]
a tool to “piggyback” onto YOUR biotinylated material via streptavidin; targeting cells that recognize YOUR biotinylated material, eliminated via saporin
Streptavidin-ZAP [IT-27] Read More »
a tool to “piggyback” onto YOUR biotinylated material via streptavidin; targeting cells that recognize YOUR biotinylated material, eliminated via saporin
Streptavidin-ZAP [IT-27] Read More »
Streptavidin-ZAP[Streptavidin conjugated to Saporin](Cat. #IT-27) Objective: To determine if an immunotoxin cocktail targeted to multiple epitopes has synergistic effects on low expression level cells, which would expand the applicable range of immunotoxin therapy for cancer. Summary: The combination of immunotoxins with different mechanisms of action in an antibody cocktail will increase cytotoxic activities and decrease
Streptavidin-Saporin key ingredient in Immunotoxin Cocktail Read More »
Conservation of Oncofetal Antigens on Human Embryonic Stem Cells Enables Discovery of Monoclonal Antibodies against Cancer. Tan HL, Yong C, Tan BZ, Fong WJ, Padmanabhan J, Chin A, Ding V, Lau A, Zheng L, Bi X, Yang Y, & Choo A. (2018). Scientific Reports, 8 (1):11608. This study used both Hum-ZAP (Cat. #IT-22) and Streptavidin-ZAP (Cat. #IT-27)
Screening antibodies for cancer therapeutics Read More »
Top Five Targeted Toxins #1. 192-IgG-SAP (192-Saporin) (Cat. #IT-01) targets cells expressing rat p75NTR #2. Anti-DBH-SAP (Cat. #IT-03) targets cells expressing rat dopamine beta-hydroxylase (DBH) #3. mu p75-SAP (Cat. #IT-16) targets cells expressing mouse p75NTR #4. IB4-SAP (Cat. #IT-10) targets cells expressing α-D-galactopyranoside residues #5. Mac-1-SAP mouse/human (Cat. #IT-06) targets cells expressing mouse / human
Targeting Tools: Top Twenty Read More »
Characterization of the First Fully Human Anti-Tem1 Scfv in Models of Solid Tumor Imaging and Immunotoxin-Based Therapy. Yuan, X, Yang, M, Chen, X, et al. (2018). Cancer Immunol Immunother 2018/01/10. Streptavidin-ZAP (Cat. #IT-27) Objective: ScFv78 was conjugated with the ribosome-inactivating protein saporin (Streptavidin-ZAP) to evaluate whether scFv78 may be used as a vehicle for theTEM1-targeted
Streptavidin-ZAP evaluates antibodies for targeted tumor treatment Read More »
Q: I recently tried to order avidinylated-SAP (Cat. #IT-09) and was told that this product has been replaced with a new product, Streptavidin-ZAP (Cat. #IT-27). Why did you replace avidinylated-SAP? A: We initially had good results with avidinylated-SAP. It combined well with biotinylated antibody to produce extremely potent cytotoxic materials, and had low toxicity itself.
Streptavidin versus Avidin Read More »
Bostad M, Kausberg M, Weyergang A, Olsen C, Berg K, Høgset A, Selbo P (2014) Light-triggered, efficient cytosolic release of IM7-saporin targeting the putative cancer stem cell marker CD44 by photochemical internalization. Mol Pharm 11:2764-2776. doi: 10.1021/mp500129t Summary: CD44 is known as a common cancer stem cell (CSC) marker. Given that CSC’s seem to have
Ha K, Bidlingmaier S, Zhang Y, Su Y, Liu B (2014) High-content analysis of antibody phage-display library selection outputs identifies tumor selective macropinocytosis-dependent rapidly internalizing antibodies. Mol Cell Proteomics 13:3320-3331. doi: 10.1074/mcp.M114.039768 Summary: Macropinocytosis, the internalization of large endocytic vesicles called macropinosomes, is upregulated in Ras-transformed cancers. To date, large-scale antibody generation strategies have not
Elson-Schwab L, Garner OB, Schuksz M, Esko JD, Tor Y (2007) Guanidinylated-Neomycin delivers large, bioactive cargo into cells through a heparan sulfate dependent pathway. J Biol Chem 282(18):13585-13591. doi: 10.1074/jbc.M700463200 Summary: The uptake of high molecular weight drugs into cells is a stumbling block for some potential therapeutics. Using a neomycin derivative in which guanidinium
Yuan X, Yang M, Chen X, Zhang X, Sukhadia S, Musolino N, Bao H, Chen T, Xu C, Wang Q, Santoro S, Ricklin D, Hu J, Lin R, Yang W, Li Z, Qin W, Zhao A (2017) Characterization of the first fully macropinocytosing human antibodies human anti-TEM1 scFv in models of solid tumor imaging and