06q4

Targeting Trends 2006 Newsletter

Read the 2006 Newsletters and recent scientific references 2006, issue 1: The Biologically Active Cholecystokinin (26-33) peptide, [Tyr2-SO3]CCK-8, Retains High Affinity for CCK2 Receptors after Covalent Conjugation to Saporin / by Lai J, Zhang W, Badghisi H, Hruby VJ, Porreca F / featuring CCK-SAP (Cat. #IT-31) SFN Poster of the Year Targeting Talk: LPS Content …

Targeting Trends 2006 Newsletter Read More »

Targeting Tools: Anti-6-His

Anti-6-His Mouse Monoclonal, Cat. #AB-20 [see also Cat. #AB-213] The use of polyhistidine tags has become a popular method for protein purification, commonly used in the screening process as a tag for your protein or peptide of interest. Whether the material you are screening for is affinity purified or in crude bacterial extract, you will …

Targeting Tools: Anti-6-His Read More »

Targeting Tools: HRP-labeled Saporin

HRP-labeled Saporin Goat Polyclonal, Cat. #AB-15HRP HRP-labeled Anti-SAP can be used to verify binding specificity of a targeted toxin to a cell line expressing the target molecule. By first binding the targeted toxin to protein extract or plate-bound antigen, then binding HRP-labeled Anti-SAP to the targeted toxin, specificity can be confirmed through the use of …

Targeting Tools: HRP-labeled Saporin Read More »

Cover Article: Basomedial Hypothalamic Injections of Neuropeptide Y Conjugated to Saporin Selectively Disrupt Hypothalamic Controls of Food Intake

This article is a summary of data presented in reference #1. Figures 1-4 are taken from that article. This work was funded by NS045520 and DK40498 to S. Ritter. Neuropeptide Y (NPY) conjugated to saporin (SAP), a ribosomal toxin, is a compound designed to selectively target and lesion NPY receptor-expressing cells. We conducted competitive binding …

Cover Article: Basomedial Hypothalamic Injections of Neuropeptide Y Conjugated to Saporin Selectively Disrupt Hypothalamic Controls of Food Intake Read More »