It’s routine for primary neuronal cultures to have “contamination” with naturally occurring microglia. But what happens when you don’t want these little creatures or the cytokines and cytotoxins that they release? Or you want to eliminate any effect that microglia have on neurons or astrocytes? ATS has the cheap, effective and very simple solution for […]
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Chronic pain conditions are often caused by ongoing disease states or tissue damage that create sensitization of both the primary afferents (nerves that convey impulses from the outer part of the body) and spinal cord neurons. This sensitization results in a persistent increased sensitivity to both noxious (hyperalgesia) and non-noxious (allodynia) stimuli. Many persistent pain
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Contributed by Dr. Fiorenzo Stirpe, Dipartimento di Patologia sperimentale, Università di Bologna, I-40125 Bologna, Italy Saporin from Saponaria officinalis (soapwort plant of the Caryophyllaceae family) was discovered as part of research on plant toxins we undertook around 1970, when we became interested in the study of the mechanism of action of ricin. Ricin, from Ricinus
Cover Article: The Discovery of Saporin Read More »
Advanced Targeting Systems has introduced important new toxins for investigation into cells that express the interleukin-2 (IL-2) receptor and especially the workings of the immune system. Anti-CD25-saporin (Anti-Tac-SAP) conjugates are directed to the human IL-2 receptor or to the mouse IL-2 receptor. This marker for activated T lymphocytes allows removal of this important group of
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We have re-designed the anti-murine p75-Saporin targeted toxin (mu p75-SAP, Cat. #IT-16) and have produced a conjugate that is much more potent in our in vitro cell cytotoxicity assays. Previously, we used a rat monoclonal antibody. This antibody had been outperformed by our rabbit polyclonal AB-N01, in several assays, especially FACS analysis of murine p75-expressing
Targeting Tools: mu p75-SAP Improved Read More »
Contributed by Dr. James Smith, Wake Forest University School of Medicine, Department of Physiology and Pharmacology, Medical Center Blvd, Winston-Salem NC 27157 Recent data implicate cholinergic neurons in the brain processes that underlie reinforcement. The involvement of cholinergic neurons in cocaine self-administration has been recently demonstrated using muscarinic and nicotinic agonists and antagonists, microdialysis, assessment
Advanced Targeting Systems is pleased to announce the availability of polyclonal antibodies to somatostatin receptor (SSTr) sub-types 1 and 4. SSTr1 antiserum, Cat. #AB-N19 SSTr1 affinity-purified, Cat. #AB-N20AP SSTr4 affinity-purified, Cat. #AB-N21AP Antibody to SSTr1 SSTr1 is a 391 amino acid G-protein-coupled receptor that contains three glycosylation sites and that is expressed in several areas
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Progress continues to be made in moving SP-SAP closer to clinical trials as a treatment for chronic pain. The latest development is the leasing of a 3700-sq ft facility adjacent to the current ATS facility in San Diego. This facility will be converted to GMP (Good Manufacturing Practice) space suitable for production of SP-SAP for
ATS Gets New Facility for SP-SAP Production Read More »
Contributed by ATS’s 2003 Society for Neuroscience Poster of the Year Award Winner:Dr. Jill McGaughy, Boston University, 64 Cummington St., Boston, MA 02215 Muscarinic cholinergic receptor activation in entorhinal cortex (EC) activates intrinsic depolarizing membrane currents, which cause self-sustained spiking activity in single neurons.[1] This effect may underlie delay activity and match-dependent activity changes in
Read the 2003 Newsletters and recent scientific references 2003, issue 1: Deep Lumbar Neurons Control Ejaculation / by Truitt W / featuring SSP-SAP, Cat. #IT-11 SFN Poster of the Year SP-SAP as a Chronic Pain Therapeutic: An Update Targeting Talk: Custom Peptide-Saporin Conjugates Targeting Tools: Dermorphin-SAP (Cat. #IT-12) 2003, issue 2: Biotinylated targeting: A viable
Targeting Trends 2003 Newsletter Read More »