antibodies

Berson DM, Castrucci AM, Provencio I (2010) Morphology and mosaics of melanopsin-expressing retinal ganglion cell types in mice. J Comp Neurol 518(13):2405-2422. doi: 10.1002/cne.22381 PMID: 20503419

Objective: To provide a fuller description of murine cell types expressing melanopsin, their contribution to the plexuses of melanopsin dendrites, and mosaics formed by each type.

Summary: M1 cells, corresponding to the originally described ganglion-cell photoreceptors, occupy the ganglion cell or inner nuclear layers. M2 cells ramify in the inner third of the IPL. Rare bistratified cells deploy terminal dendrites within both melanopsin-immunoreactive plexuses within the ON sublayer.

Usage: Immunohistochemistry: The tissue was incubated for 24 hr at 4°C in unpurified primary antiserum diluted 1:2500. Immunoperoxidase labeling of flat mount tissue was incubated for 3 days in the unpurified primary antiserum diluted 1:2500.

Related Products: Melanopsin Rabbit Polyclonal (Cat. #AB-N38)

Schnitzler AC, Mellott TJ, Lopez-Coviella I, Tallini YN, Kotlikoff MI, Follettie MT, Blusztajn JK (2010) BMP9 (bone morphogenetic protein 9) induces NGF as an autocrine/paracrine cholinergic trophic factor in developing basal forebrain neurons. J Neurosci 30(24):8221-8228. doi: 10.1523/JNEUROSCI.5611-09.2010 PMID: 20554873

Summary: Bone morphogentic protein (BMP) 9 is a cholinergic differentiation factor that increases acetylcholine synthesis and choline acetyltransferase gene expression. The authors investigated whether BMP9 could induce cholinergic trophic factors in murine septal cells. One experiment involved the sorting of E18 septal cells using anti-p75 (Cat. #AB-N01AP, 5µg/2×106 cells). The increased Ngf gene expression in response to BMP9 in p75-positive basal forebrain cholinergic neurons indicates an autocrine/paracrine role for NGF in the development and maintenance of these cells.

Related Products: NGFr (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Adachi T, Huxtable AG, Fang X, Funk GD (2010) Substance P modulation of hypoglossal motoneuron excitability during development: changing balance between conductances. J Neurophysiol 104(2):854-872. doi: 10.1152/jn.00016.2010 PMID: 20538779

Summary: This work examined how neuromuscular networks that are immature, but functional, at birth move through development while remaining operational. The authors focused on hypoglossal motoneurons involved in behaviors such as swallowing, suckling, and breathing. Immunohistochemistry was performed using an anti-NK-1 receptor antibody (Cat. #AB-N04). The data show that although NK-1 receptor density decreases as the animal matures, substance P (the NK-1 receptor ligand) remains an important part of these networks.

Related Products: Antibody to NK-1 Receptor (Cat. #AB-N04)

Gonzalez-Menendez I, Contreras F, Cernuda-Cernuda R, Provencio I, Garcia-Fernandez JM (2010) Postnatal development and functional adaptations of the melanopsin photoreceptive system in the albino mouse retina. Invest Ophthalmol Vis Sci 51(9):4840-4847. doi: 10.1167/iovs.10-5253 PMID: 20435589

Summary: Melanopsin-expressing intrinsically photosensitive retinal ganglion cells (ipRGCs) adjust the circadian pacemaker of mammals by detecting light. The authors tracked the development of ipRGCs in postnatal mice under varying light conditions. Immunohistochemistry for these experiments was done using an anti-mouse melanopsin polyclonal antibody (Cat. #AB-N38). Alteration of the standard light/dark cycle clearly affected the development of ipRGCs.

Related Products: Melanopsin Rabbit Polyclonal (Cat. #AB-N38)

Lee G, Chambers SM, Tomishima MJ, Studer L (2010) Derivation of neural crest cells from human pluripotent stem cells. Nat Protoc 5(4):688-701. doi: 10.1038/nprot.2010.35 PMID: 20360764

Summary: Protocols are presented for the purification and propagation of hPSC-NC cells using flow cytometry and defined in vitro culture conditions. This protocol has been validated in multiple independent hESC and hiPSC lines. The average time required for generating purified hPSC-NC precursors using this protocol is 2–5 weeks.

Usage: Neural crest cells (1:200).

Related Products: NGFr (ME20.4, p75) Mouse Monoclonal (Cat. #AB-N07)

Q: We’re interested in trying out your melanopsin antibody (Cat. #AB-N38) using immunohistochemistry in mouse retina. Do you have a recommended protocol?

A:  This protocol has been utilized successfully with anti-melanopsin.

Panda S. et al. 2002. Melanopsin (Opn4) requirement for normal light- induced circadian phase shifting. Science 298(5601):2213-2216.

Related: Anti-Melanopsin (Cat. #AB-N38), Anti-Melanopsin, affinity-purified (Cat. #AB-N39)

Marshall AM, Nommsen-Rivers LA, Hernandez LL, Dewey KG, Chantry CJ, Gregerson KA, Horseman ND (2010) Serotonin transport and metabolism in the mammary gland modulates secretory activation and involution. J Clin Endocrinol Metab 95(2):837-846. doi: 10.1210/jc.2009-1575 PMID: 19965920

Summary: Serotonin is known to be a local regulator of lactation homeostasis. This work examined  the roles of the serotonin reuptake transporter (SERT) and monoamine oxidase in this system. Immunohistochemical and immunocytochemical staining was done on human primary mammary epithelial cells and mouse tissue with an anti-SERT antibody (Cat. #AB-N09). Additional data included epidemiological studies and selective serotonin reuptake inhibitor treatment of mice. The results suggest that women taking SSRI inhibitor medications were more likely to experience delayed secretory activation.

Related Products: Antibody to Serotonin Transporter (SERT, Cat. #AB-N09)

Riedl MS, Braun PD, Kitto KF, Roiko SA, Anderson LB, Honda CN, Fairbanks CA, Vulchanova L (2009) Proteomic analysis uncovers novel actions of the neurosecretory protein VGF in nociceptive processing. J Neurosci 29:13377-13388. doi: 10.1523/JNEUROSCI.1127-09.2009 PMID: 19846725

Summary: Peripheral tissue injury can alter protein expression in sensory neurons, which may contribute to abnormal nociceptive processing. The authors used cultured dorsal root ganglion neurons as a model for axotomized neurons to examine early changes in protein expression after nerve injury. Several different parameters were measured, including immunohistochemistry using anti-TrkA (Cat. #AB-N03). The data show an increased level of a putative neuropeptide precursor, VGF, as a result of nerve injury.

Related Products: trkA Rabbit Polyclonal (Cat. #AB-N03)

Steiner J, Carneiro A, Wright J, Matthies H, Prasad H, Nicki C, Dostmann W, Buchanan C, Corbin J, Francis S, Blakely R (2009) cGMP-dependent protein kinase Ialpha associates with the antidepressant-sensitive serotonin transporter and dictates rapid modulation of serotonin uptake. Mol Brain 2:26. doi: 10.1186/1756-6606-2-26 PMID: 19656393

Summary: The neurotransmitter serotonin fulfills an important modulatory role in a wide range of brain functions including mood, appetite, sexual behavior, and reward. Serotonin transporters (SERT) are involved in the inactivation of synaptic serotonin, as well as serotonin recycling, which is critical to the maintenance of neuronal serotonin stores. In this work the authors examined how neuronal A3 adenosine receptor activation can enhance presynaptic serotonin transport in vitro as well as SERT-mediated clearance in vivo. The in vitro experiments included immunohistochemistry with anti-SERT (Cat. #AB-N40) on RN46A cells at a 1:500 dilution.

Related Products: SERT Mouse Monoclonal (Cat. #AB-N40)

Rock JR, Onaitis MW, Rawlins EL, Lu Y, Clark CP, Xue Y, Randell SH, Hogan BL (2009) Basal cells as stem cells of the mouse trachea and human airway epithelium. Proc Natl Acad Sci U S A 106(31):12771-12775. doi: 10.1073/pnas.0906850106 PMID: 19625615

Related Products: NGFr (mu p75) Rabbit Polyclonal (Cat. #AB-N01)