antibodies

Takasusuki T, Yaksh TL (2011) The effects of intrathecal and systemic gabapentin on spinal substance P release. Anesth Analg 112(4):971-976. doi: 10.1213/ANE.0b013e31820f2a16 PMID: 21385982

Summary: Intrathecal or systemically-administered gabapentin is an antihyperalgesic. Given that gabapentin binds a voltage-sensitive calcium channel and that some of these channels regulate substance P (SP) release, the authors investigated whether gabapentin affects SP levels. Immunohistochemistry was done in rats following a gabapentin/formalin pain model. A neurokinin-1 receptor antibody (Cat. #AB-N04) was used to quantitate NK1r, and therefore assess SP activity. It was found that both spinal and systemic gabapentin inhibit SP release from small, primary afferents.

Related Products: Antibody to NK-1 Receptor (Cat. #AB-N04)

Chen AI, Nguyen CN, Copenhagen DR, Badurek S, Minichiello L, Ranscht B, Reichardt LF (2011) TrkB (Tropomyosin-related kinase B) controls the assembly and maintenance of GABAergic synapses in the cerebellar cortex. J Neurosci 31(8):2769-2780. doi: 10.1523/JNEUROSCI.4991-10.2011 PMID: 21414899

Summary: In this work the authors investigated the role of TrkB in GABAergic inhibitory synapses in the mouse cerebellar cortex. Using a variety of techniques, including immunohistochemistry utilizing an mGluR2 antibody (Cat. #AB-N32), it was shown that TrkB is required for both assembly and maintenance of these synapses. The primary role of TrkB appears to be regulating the localization of synaptic constituents.

Related Products: Metabotropic Glutamate Receptor 2 (mGluR2) Mouse Monoclonal (Cat. #AB-N32)

Montandon G, Qin W, Liu H, Ren J, Greer JJ, Horner RL (2011) PreBotzinger complex neurokinin-1 receptor-expressing neurons mediate opioid-induced respiratory depression. J Neurosci 31(4):1292-1301. doi: 10.1523/JNEUROSCI.4611-10.2011 PMID: 21273414

Summary: In order to identify the neurons involved with respiratory depression due to administration of opioids, some neuro- transmission networks in the preBotzinger complex were locally manipulated. Among various techniques used to analyze the results was immunohistochemistry with anti-NK1r (Cat. #AB-N04). Results show the preBotzinger complex is responsible for suppression of respiratory rate due to opioids.

Related Products: Antibody to NK-1 Receptor (Cat. #AB-N04)

Ohta S, Imaizumi Y, Okada Y, Akamatsu W, Kuwahara R, Ohyama M, Amagai M, Matsuzaki Y, Yamanaka S, Okano H, Kawakami Y (2011) Generation of human melanocytes from induced pluripotent stem cells. PLoS One 6(1):e16182. doi: 10.1371/journal.pone.0016182 PMID: 21249204

Summary: For immunocytochemistry, cells were fixed with PBS containing 4% PFA for 20 min at room temperature. Then, cells were subjected to immunofluorescence staining (1∶100).

Related Products: NGFr (ME20.4, p75) Mouse Monoclonal (Cat. #AB-N07)

Wagle M, Mathur P, Guo S (2011) Corticotropin-releasing factor critical for zebrafish camouflage behavior is regulated by light and sensitive to ethanol. J Neurosci 31(1):214-224. doi: 10.1523/JNEUROSCI.3339-10.2011 PMID: 21209207

Objective: To explore neural circuit assembly and function using the hardwired camouflage response of zebrafish.

Summary: Both light exposure and ethanol affect the camouflage response. Understanding this system could provide a tool to further investigate the effect of alcohol on neural circuits.

Usage: Immunostaining (1:500)

Related Products: Corticotropin Releasing Hormone Rabbit Polyclonal (Cat. #AB-02)

Marshall RS, D’Avila F, Di Cola A, Traini R, Spano L, Fabbrini MS, Ceriotti A (2011) Signal peptide-regulated toxicity of a plant ribosome-inactivating protein during cell stress. Plant J 65(2):218-29. doi: 10.1111/j.1365-313X.2010.04413.x PMID: 21223387

Summary: Type I ribosome inactivating proteins (RIPs) are thought to have a role in defending plants against viral or fungal infections. Most type I RIPs have signal peptides for insertion into the endoplasmic reticulum, followed by transportation to a vacuole or the cell wall. The authors examined signal peptide regulation under stress in tobacco plants transfected with saporin. One method of analysis was western blots using anti-saporin (Cat. #AB-15).

Related Products: Saporin Goat Polyclonal (Cat. #AB-15)

Girard BM, Malley SE, Vizzard MA (2011) Neurotrophin/receptor expression in urinary bladder of mice with overexpression of NGF in urothelium. Am J Physiol Renal Physiol 300(2):F345-55. doi: 10.1152/ajprenal.00515.2010 PMID: 21048026

Summary: Chronic overexpression of nerve growth factor (NGF) in the urinary bladder results in neuronal sprouting, increased voiding frequency, and referred somatic hypersensitivity. The authors investigated several growth factor and receptor responses to chronic overexpression of NGF. A p75 antibody (Cat. #AB-N01) was used to obtain the immunohistochemistry data. The data suggest that changes due to NGF overexpression may be a compensatory attempt to reduce voiding frequency.

Related Products: NGFr (mu p75) Rabbit Polyclonal (Cat. #AB-N01)

Gray PA, Hayes JA, Ling GY, Llona I, Tupal S, Picardo MCD, Ross SE, Hirata T, Corbin JG, Eugenin J, Del Negro CA (2010) Developmental origin of preBotzinger Complex respiratory neurons. J Neurosci 30(44):14883-14895. doi: 10.1523/JNEUROSCI.4031-10.2010 PMID: 21048147

Summary: Breathing in adult rats requires a subset of neurons in the preBötzinger Complex (preBötC) that express the neurokinin-1 receptor and the peptide somatostatin. In this work the authors investigate the developmental origins of these cells. Using various techniques, including immunohistochemistry with an anti-neurokinin-1 receptor antibody (Cat. #AB-N04), it is demonstrated that neurons derived from Dbx-1 expressing progenitor cells are important in the generation of respiratory behavior.

Related Products: Antibody to NK-1 Receptor (Cat. #AB-N04)

Ariyama H, Kono N, Matsuda S, Inoue T, Arai H (2010) Decrease in membrane phospholipid unsaturation induces unfolded protein response. J Biol Chem 285(29):22027-22035. doi: 10.1074/jbc.M110.126870 PMID: 20489212

Summary: Properties of the cell membrane can be influenced by the degree of fatty acid unsaturation in membrane phospholipids. Alteration of this unsaturation has been implicated in many disease states. Using an anti-SCD1 antibody (Cat. #AB-259) to visualize SCD-1 levels by western blot, the authors determined that there are several genetic factors that affect the level of saturated fatty acid in systems modulating insulin resistance, type 2 diabetes, and cardiovascular disease.

Related Products: SCD-1 Mouse Monoclonal (Cat. #AB-259)

Lappi DA, Ancheta LR, Nowak RA (2010) Featured Article: Internalization of basigin-2, a poor prognosis marker, by a monoclonal antibody. Targeting Trends 11(3) PMID: 0

Related Products: Antibody to Basigin (Cat. #AB-42), Anti-Basigin2-SAP (Cat. #IT-54)

Read the featured article in Targeting Trends.