antibodies

Gibon J, Buckley S, Unsain N, Kaartinen V, Séguéla P, Barker P (2015) proBDNF and p75NTR control excitability and persistent firing of cortical pyramidal neurons. J Neurosci 35:9741-9753. doi: 10.1523/JNEUROSCI.4655-14.2015 PMID: 26134656

Summary: Principal neurons in the entorhinal cortex (EC) display persistent firing (PF) during working-memory tasks. Much of the communication between the hippocampus and the neocortex passes through the EC, and the EC also receives some cholinergic input from the medial septum and diagonal band of Broca. In this work the authors investigated the role of pro-brain-derived neurotrophic factor (proBDNF) and the p75 receptor in excitability and PF in the EC. The authors propose the proBDNF/p75 system as a regulator for pyramidal neuron excitability and PF in the EC, preventing runaway activity. Some of the western blot and current-clamp data was generated using Anti-p75 (Cat. #AB-N01; no concentration information provided).

Related Products: NGFr (mu p75) Rabbit Polyclonal (Cat. #AB-N01)

El-Danaf R, Huberman A (2015) Characteristic patterns of dendritic remodeling in early-stage glaucoma: evidence from genetically identified retinal ganglion cell types. J Neurosci 35:2329-2343. doi: 10.1523/JNEUROSCI.1419-14.2015 PMID: 25673829

Summary: The loss of retinal ganglion cells (RGC) is the second-most common cause of blindness worldwide. Using several mouse transgenic cell lines, the authors investigated the changes that occur on the establishment of elevated ocular pressure. Anti-melanopsin (Cat. #AB-N39) at 1:1000 was used to illuminate the morphology of the M1 intrinsically photosensitive RGC.

Related Products: Melanopsin Rabbit Polyclonal, affinity-purified (Cat. #AB-N39)

Ma R, Latif R, Davies TF (2015) Human embryonic stem cells form functional thyroid follicles. Thyroid 25(4):455-461. doi: 10.1089/thy.2014.0537 PMID: 25585054

Summary: Lysates were prepared from single- and double-transfected cells by treating with 1 ·RIPA buffer for 20 min on ice. The samples were then centrifuged and treated with 5 · sample buffer at 100°C for 5 min and loaded on a 4–15% SDS-PAGE gel. Western blotting was performed according to a standard protocol.

Related Products: TSH Receptor (A10) Mouse Monoclonal (Cat. #AB-N16)

Mao C, Li H, Zhang Z, Kiyama T, Panda S, Hattar S, Ribelayga C, Mills S, Wang S (2014) T-box transcription regulator Tbr2 is essential for the formation and maintenance of Opn4/melanopsin-expressing intrinsically photosensitive retinal ganglion cells. J Neurosci 34:13083-13095. doi: 10.1523/JNEUROSCI.1027-14.2014 PMID: 25253855

Summary: Opsin 4/melanopsin-expressing intrinsically photosensitive retinal ganglion cells (ipRGCs) are responsible for controlling non-image-forming visual functions in the retina. The findings show that opsin 4 is only expressed in Tbr2-positive ipRGCs, no ipRGCs are found if Tbr2 is deleted before RGC specialization, and most ipRGCs are eliminated when Tbr2 is deleted from established ipRGCs. An antibody against melanopsin (Cat. #AB-N39) was used at a 1:1000 dilution for immunohistochemical analyses.

Related Products: Melanopsin Rabbit Polyclonal, affinity-purified (Cat. #AB-N39)

Luo W, Lin B, Wang Y, Zhong J, O’Meally R, Cole R, Pandey A, Levchenko A, Semenza G (2014) PHD3-mediated prolyl hydroxylation of nonmuscle actin impairs polymerization and cell motility. Mol Biol Cell 25:2788-2796. doi: 10.1091/mbc.E14-02-0775 PMID: 25079693

Usage: Western blot

Related Products: Trans-4-Hydroxy-L-Proline Rabbit Polyclonal, Conjugated (Cat. #AB-T044)

Marbiah MM, Harvey A, West BT, Louzolo A, Banerjee P, Alden J, Grigoriadis A, Hummerich H, Kan HM, Cai Y, Bloom GS, Jat P, Collinge J, Klöhn PC (2014) Identification of a gene regulatory network associated with prion replication. EMBO J 33(14):1527-1547. doi: 10.15252/embj.201387150 PMID: 24843046

Related Products: Antibody to IQGAP2 (Cat. #AB-V28)

Alvares T, Revill A, Huxtable A, Lorenz C, Funk G (2014) P2Y1 receptor-mediated potentiation of inspiratory motor output in neonatal rat in vitro. J Physiol 592:3089-3111. doi: 10.1113/jphysiol.2013.268136 PMID: 24879869

Summary: P2YR’s are metabotropic purinergic receptors found in some parts of the CNS. A subtype of this receptor excites rhythm generating networks in the preBötzinger complex. In order to better understand the role of these receptors in modulation of motor output the authors used brainstem-spinal cord and medullary slice preparations from neonatal rats to investigate P2Y1R signaling on specific neurons that innervate diaphragm and airway muscles. Anti-NK1r (Cat. #AB-N33AP) at a 1:1000 dilution was used during the immunohistochemistry. The data suggest that loss of purinergic modulation contributes to motoneuron excitability.

Related Products: NK-1 Receptor Rabbit Polyclonal, affinity-purified (Cat. #AB-N33AP)

Zheng X, Zhai B, Koivunen P, Shin S, Lu G, Liu J, Geisen C, Chakraborty A, Moslehi J, Smalley D, Wei X, Chen X, Chen Z, Beres J, Zhang J, Tsao J, Brenner M, Zhang Y, Fan C, DePinho R, Paik J, Gygi S, Kaelin W, Zhang Q (2014) Prolyl hydroxylation by EglN2 destabilizes FOXO3a by blocking its interaction with the USP9x deubiquitinase. Genes Dev 28:1429-1444. doi: 10.1101/gad.242131.114 PMID: 24990963

Summary: Members of the FOXO family are thought to act as tumor suppressor genes. In this work the authors investigated the hydroxylation of FOXO3a by EglN2. This hydroxylation pushes FOXO3a toward a protosomal degradation pathway. Loss of FOXO3a in turn allows the accumulation of Cyclin D1, which has been found to be overexpressed in some breast cancers. Some of the data were generated using immunoblots with anti-transhydroxylated proline (Cat. #AB-T044).

Usage: Western blot

Related Products: Trans-4-Hydroxy-L-Proline Rabbit Polyclonal, Conjugated (Cat. #AB-T044)

Joly S, Guzik-Kornacka A, Schwab M, Pernet V (2014) New mouse retinal stroke model reveals direction-selective circuit damage linked to permanent optokinetic response loss. Invest Ophthalmol Vis Sci 55:4476-4489. doi: 10.1167/iovs.14-14521 PMID: 24970264

Summary: The authors used a mouse model of ‘retinal stroke’ to better delineate the optokinetic response deficits at the cellular level. Damage was found in the processes of starburst amacrine cells (SACs), and to a lesser extent, the dendrites. Anti-melanopsin (Cat. #AB-N38) at 1:2500 was used for immunohistochemistry.

Related Products: Melanopsin Rabbit Polyclonal (Cat. #AB-N38)

Bajo V, Leach N, Cordery P, Nodal F, King A (2014) The cholinergic basal forebrain in the ferret and its inputs to the auditory cortex. Eur J Neurosci 40:2922-2940. doi: 10.1111/ejn.12653 PMID: 24945075

Summary: The ferret has become a more common animal model in auditory neuroscience. Unlike rodent models, however, anatomical data describing the organization of the basal forebrain cholinergic system and its projections to the auditory cortex have not been well characterized. Using a variety of methods the authors mapped the architecture of the ferret basal forebrain. IHC was done with several antibodies including anti-ChAT (Cat. #AB-N34AP; 1:1000) and anti-NGFr (Cat. #AB-N07; 1:500). Animals also received 17 μg of ME20.4-SAP (Cat. #IT-15) in a total of 17 injections into the ectosylvian gyrus. The results indicate that acetylcholine is most likely involved in modulation of auditory processing.

Related Products: Choline Acetyltransferase Rabbit Polyclonal, affinity-purified (Cat. #AB-N34AP), NGFr (ME20.4, p75) Mouse Monoclonal (Cat. #AB-N07), ME20.4-SAP (Cat. #IT-15)