References

Nagarkoti S, Dubey M, Awasthi D, Kumar V, Chandra T, Kumar S, Dikshit M (2018) S-Glutathionylation of p47phox sustains superoxide generation in activated neutrophils. Biochim Biophys Acta Mol Cell Res 1865(2):444-454. doi: 10.1016/j.bbamcr.2017.11.014 PMID: 29195919

Objective: To investigate the role of S-Glutathionylation of p47phox.

Summary: In Phorbol 12-myristate 13-acetate (PMA) or Nitric oxide (NO) treated neutrophils a subunit of NOX2, p47phox gets glutathionylated to sustain ROS generation along with a decrease in catalase, Grx-1 activity and change in GSH/GSSG ratio.

Usage: Western blot

Related Products: NO-L-Cysteine Mouse Monoclonal, Conjugated (Cat. #AB-T125)

Park HW, Kim H-L, Park YS, Kim I-B (2018) The transient intermediate plexiform layer, a plexiform layer-like structure temporarily existing in the inner nuclear layer in developing rat retina. Exp Neurobiol 27:28-33. doi: 10.5607/en.2018.27.1.28

Related Products: SSP-SAP (Cat. #IT-11)

Lee SJ, Jokiaho AJ, Sanchez-Watts G, Watts AG (2018) Catecholaminergic projections into an interconnected forebrain network control the sensitivity of male rats to diet-induced obesity. Am J Physiol Regul Integr Comp Physiol 314(6):R811-R823. doi: 10.1152/ajpregu.00423.2017

Objective: To investigate the role of hindbrain catecholamine neuron pathways and their contribution to long-term energy homeostasis by controlling obesogenic sensitivity to a high-fat, high sucrose choice diet.

Summary: The authors show that catecholamine neurons (primarily in the VLM and NTS) convey essential feedback signals to enable long-term adaptive control of energy metabolism when animals consume a predominantly carbohydrate diet. This is the first report specifically associating this projection system with the long-term control of adiposity.

Usage: Catecholaminergic projections to the PVH and related parts of the forebrain were lesioned with bilateral injections each consisting of 42 ng/200 nL of Anti-DBH-SAP or equimolar amounts of control Mouse IgG-SAP.

Related Products: Anti-DBH-SAP (Cat. #IT-03), Mouse IgG-SAP (Cat. #IT-18)

Zhou H, Huang J, Zhu L, Cao Y (2018) Erythropoietin alleviates post-resuscitation myocardial dysfunction in rats potentially through increasing the expression of angiotensin II receptor type 2 in myocardial tissues. Mol Med Rep 17:5184-5192. doi: 10.3892/mmr.2018.8473 PMID: 29393490

Objective: To determine whether erythropoietin (EPO) improves post‑resuscitation myocardial dysfunction and how it affects the renin‑angiotensin system.

Summary: The present study confirmed that EPO treatment is beneficial for protecting cardiac function post‑resuscitation, and the roles of EPO in alleviating post‑resuscitation myocardial dysfunction may potentially be associated with enhanced myocardial expression of AT2R.

Usage: Western blot analysis of AT-1R (1:500) in the myocardium

Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP)

Shim HS, Park H-J, Lee M-S, Ye M, Shim I (2018) The role of the supramammillary area of the hypothalamus in cognitive functions. Animal Cells and Systems 22:37-44. doi: 10.1080/19768354.2018.1427627

Related Products: 192-IgG-SAP (Cat. #IT-01)

Dobryakova YV, Kasianov A, Zaichenko MI, Stepanichev MY, Chesnokova EA, Kolosov PM, Markevich VA, Bolshakov AP (2018) Intracerebroventricular administration of 192IgG-saporin alters expression of microglia-associated genes in the dorsal but not ventral hippocampus. Front Mol Neurosci 10:429. doi: 10.3389/fnmol.2017.00429

Objective: To analyze the postponed consequences of cholinergic deficit in different parts of the hippocampus.

Summary: Disturbance of memory-associated behavior after administration of 192-IgG-SAP is associated with upregulation of microglia-associated genes in the dorsal but not ventral hippocampus.

Usage: Rats received bilateral i.c.v.infusions of 192-IgG-SAP (4 mcg/site).

Related Products: 192-IgG-SAP (Cat. #IT-01)

Grillo SL, Stella SL, Jr. (2018) Melanopsin retinal ganglion cells are not labeled in Thy-1YFP-16 transgenic mice. Neuroreport 29:118-122. doi: 10.1097/WNR.0000000000000918 PMID: 29251688

Objective: To determine whether mRGCs are labeled with YFP in Thy-1 YFP-16 transgenic mice. The transgenic Thy-1 YFP mouse line 16 (Thy-1 YFP-16) expresses yellow-fluorescent protein (YFP) in projection neurons, including RGCs.

Summary: The majority of mRGC somas and axons are not labeled with YFP in the transgenic Thy-1 YFP-16 mouse line; therefore, this mouse model may not suitable for research involving mRGC visual pathways.

Usage: Affinity purified rabbit polyclonal antibody raised against melanopsin (1:500) was used to label mRGCs (Retinal ganglion cells expressing melanopsin). Immunolabeled Thy-1 YFP-16 mouse retina with anti-melanopsin and found that majority (∼89%) of mRGCs are not YFP-positive despite numerous other YFP-positive RGCs in the retina.

Related Products: Melanopsin Rabbit Polyclonal, affinity-purified (Cat. #AB-N39)

Liu W, Zhao Y, Zhang X, Ji J (2018) Simvastatin ameliorates cognitive impairments via inhibition of oxidative stress‑induced apoptosis of hippocampal cells through the ERK/AKT signaling pathway in a rat model of senile dementia. Mol Med Rep 17(1):1885-1892. doi: 10.3892/mmr.2017.8098 PMID: 29257256

Objective: To investigate a potential mechanism underlying simvastatin activity in hippocampal cells.

Summary: The preclinical results indicate that simvastatin is efficient in preventing memory lapse and inhibiting apoptosis of hippocampal cells via the ERK/AKT signaling pathway, which may in the future improve cognitive decline and dementia in patients.

Usage: immunohistochemistry (1:500)

Related Products: NGFR (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Potter H, Alenciks E, Frazier K, Porter A, Fraley GS (2018) Immunolesion of melanopsin neurons causes gonadal regression in Pekin drakes (Anas platyrhynchos domesticus). Gen Comp Endocrinol 256:16-22. doi: 10.1016/j.ygcen.2017.08.006

Objective: Examine effects of loss of melanopsin in drakes.

Summary: Loss of melanopsin in PMM elicits decrease in GnRH mRNA expression, gonadal regression, and sex behaviors in drakes.

Usage: To specifically lesion melanopsin-receptive neurons, 3 μl of an anti-melanopsin-saporin conjugate (MSAP, 100 ng/ul) was injected into the lateral ventricle (n = 10). Control drakes were injected with 3 μl of equimolar unconjugated anti-melanopsin and saporin (SAP, n = 10).

Related Products: Melanopsin-SAP (Cat. #IT-44), Saporin (Cat. #PR-01)

Oliveira LM, Moreira TS, Takakura AC (2018) Raphe pallidus is not important to central chemoreception in a rat model of Parkinson’s disease. Neuroscience 369:350-362. doi: 10.1016/j.neuroscience.2017.11.038

Objective: To investigate if serotonin-expressing neurons in the Raphe pallidus/parapyramidal region (RPa/PPy) are also involved in the modulation of breathing during central chemoreception activation in a PD animal model.

Related Products: Anti-SERT-SAP (Cat. #IT-23), Saporin (Cat. #PR-01)