References

Romero-Miguel D, Lamanna-Rama N, Casquero-Veiga M, Gómez-Rangel V, Desco M, Soto-Montenegro ML (2021) Minocycline in neurodegenerative and psychiatric diseases: An update. Eur J Neurol 28(3):1056-1081. doi: 10.1111/ene.14642

Summary: Review includes the mouse animal model of neurodegenerative disease using mu p75-SAP. Biological Effects: Attenuation of cholinergic neurons loss, glial activation and transcription of pro-inflammatory mediators.

Usage: 45 mg/Kg, i.p.

See: Hunter CL et al. Minocycline protects basal forebrain cholinergic neurons from mu p75-saporin immunotoxic lesioning. Eur J Neurosci 19(12):3305-3316, 2004.

Related Products: mu p75-SAP (Cat. #IT-16)

Lind LA, Lever TE, Nichols NL (2021) Tongue and hypoglossal morphology after intralingual cholera toxin B-saporin injection. Muscle Nerve 63(3):413-420. doi: 10.1002/mus.27131

Objective: To evaluate tongue morphology and ultrastructural changes in hypoglossal neurons and nerve fibers in an inducible rat model of dysphagia.

Summary: Preliminary results indicate this model may have translational application to a variety of neurodegenerative diseases resulting in tongue dysfunction and associated dysphagia.

Usage: Rats assigned to the CTB-SAP group (n = 10) received 25 μg CTB-SAP to produce hypoglossal motor neuron death.

Related Products: CTB-SAP (Cat. #IT-14)

McDougle M, Quinn D, Diepenbroek C, Singh A, de la Serre C, de Lartigue G (2021) Intact vagal gut-brain signalling prevents hyperphagia and excessive weight gain in response to high-fat high-sugar diet. Acta Physiol (Oxf) 231(3):e13530. doi: 10.1111/apha.13530

Objective: To assess the function of the vagus nerve lack specificity.

Summary: Intact sensory vagal neurons prevent hyperphagia and exacerbation of weight gain in response to a HFHS diet by promoting lipid-mediated satiation.

Usage: Rat nodose ganglia were injected bilaterally with either CCK-SAP or unconjugated saporin as a control.

Related Products: CCK-SAP (Cat. #IT-31)

Wang D, Shao X, Wang Q, Pan X, Dai Y, Yao S, Yin T, Wang Z, Zhu J, Xi X, Chen Z, Chen S, Zhang G (2021) Activated factor X targeted stored in platelets as an effective gene therapy strategy for both hemophilia A and B. Clin Transl Med 11(3):e375. doi: 10.1002/ctm2.375

Summary: Treatment of hemophiliacs with inhibitors remains challenging, and new treatments are in urgent need. Coagulation factor X plays a critical role in downstream blood coagulation cascade, which could serve as a bypassing agent for hemophilia therapy. Target expression of the FXa precursor to platelets can generate a storage pool of FXa in platelet α-granules, the platelet-stored FXa is effective in treating HA and HB with inhibitors, suggesting that this could be a novel choice for hemophilia patients with inhibitors.

Usage: A single dose of CD45.2-SAP (biotinylated Anti-CD45 mixed with Streptavidin-ZAP) enabled efficient engraftment of donor cells (> 90%) and full correction of sickle-cell anemia. (3 mg/kg iv; Palchaudhuri et al.).

See: Palchaudhuri R et al. Non-genotoxic conditioning for hematopoietic stem cell transplantation using a hematopoietic-cell-specific internalizing immunotoxin. Nat Biotechnol 34:738-745, 2016.

Related Products: Streptavidin-ZAP (Cat. #IT-27)

Dadashkhan S, Irani S, Bonakdar S, Ghalandari B (2021) P75 and S100 gene expression induced by cell-imprinted substrate and beta-carotene to nerve tissue engineering. Journal of Applied Polymer Science 138(26):50624. doi: 10.1002/app.50624

Objective: Aim to differentiate adipose-derived stem cells (ADSCs) to Schwann cells (SCs), as one of the major cell sources in nerve regeneration.

Summary: Use of synergistic application of imprinting method and beta-carotene BC. Schwann cell imprinted substrates can mimic the morphology and topography of SCs and induce differentiation signals in mesenchymal stem cells. BC stimulates neural differentiation.

Usage: Immunocytochemistry

Related Products: NGFr (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Wang Z, Jiang C, Yao H, Chen O, Rahman S, Gu Y, Zhao J, Huh Y, Ji RR (2021) Central opioid receptors mediate morphine-induced itch and chronic itch via disinhibition. Brain 144(2):665-681. doi: 10.1093/brain/awaa430

Summary: Itch is a common side effect of opioids, particularly as a result of epidural or intrathecal administration. Notably, morphine-elicited itch was suppressed by intrathecal administration of NPY and abolished by spinal ablation of GRPR+ neurons with intrathecal injection of Bombesin-SAP.

Usage: For ablation of GRPR+ neurons, mice were given an intrathecal injection of 400 ng Bombesin-SAP or Blank-SAP (control) 10 days before behavioral testing.

Related Products: Bombesin-SAP (Cat. #IT-40), Blank-SAP (Cat. #IT-21)

Zheng F, Chen J, Zhang X, Wang Z, Chen J, Lin X, Huang H, Fu W, Liang J, Wu W, Li B, Yao H, Hu H, Song E (2021) The HIF-1α antisense long non-coding RNA drives a positive feedback loop of HIF-1α mediated transactivation and glycolysis. Nat Commun 12(1):1341. doi: 10.1038/s41467-021-21535-3 PMID: 33637716

Objective: To investigate the contributions of HIF-1 in regulating glycolysis of cancer cells under hypoxia

Summary: HIF Antisense LncRNA (HIFAL) has a critical regulatory role in HIF-1-driven transactivation and glycolysis, identifying HIFAL as a therapeutic target of cancer treatment.

Usage: Western blot (1:500)

Related Products: Trans-4-Hydroxy-L-Proline Rabbit Polyclonal, Conjugated (Cat. #AB-T044)

Kang H, Wang Y, Zhong X, Yin W, Li F, Jiang J (2021) [Effect of salt intake on residual renal function in rats receiving peritoneal dialysis]. Nan Fang Yi Ke Da Xue Xue Bao 41(2):264-271. doi: 10.12122/j.issn.1673-4254.2021.02.15 PMID: 33624601

Objective: To assess the effect of salt intake on residual renal function in rats and explore the possible mechanism.

Summary: In rats with chronic renal failure, high salt intake promotes the activation of the renal RAS system, increases blood pressure, and aggravates renal fibrosis to accelerate the decline of residual renal function, and peritoneal dialysis partially reduces the damage of residual renal function induced by high-salt diets by removing excessive sodium.

Usage: Western Blot.

Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP)

Chen L, Ke Y, Ma H, Gao L, Zhou Y, Zhu H, Liu H, Zhang F, Zhou W (2021) Fluoxetine and ketamine reverse the depressive but not anxiety behavior induced by lesion of cholinergic neurons in the horizontal limb of the diagonal band of broca in male rat. Front Behav Neurosci 15:602708. doi: 10.3389/fnbeh.2021.602708

Summary: A lesion of horizontal limb of the diagonal band of Broca (HDB) cholinergic neurons and followed hippocampus damage may be involved in the pathogenesis of depression.

Usage: Injections of 192-IgG-SAP were made bilaterally into the HDB in a volume of 0.5 µL per side with a concentration of 0.5 µg/µL.

Related Products: 192-IgG-SAP (Cat. #IT-01)

Li Y, Lv S, Yuan H, Ye G, Mu W, Fu Y, Zhang X, Feng Z, He Y, Chen W (2021) Peripheral nerve regeneration with 3D printed bionic scaffolds loading neural crest stem cell derived Schwann cell progenitors. Advanced Functional Materials 31(16):2010215. doi: 10.1002/adfm.202010215

Objective: Regeneration efficiency and behavior of peripheral nerves are compared under three treatment strategies: 1) transplantation of Schwann cell progenitors induced from purified neural crest stem cells; 2) implantation of a multiscale scaffold based on high-resolution 3D printing; and 3) implantation of this bionic scaffold loading Schwann cell progenitors.

Summary: Implantation of multiscale scaffolds preloaded with neural crest stem cell derived Schwann cell progenitors is the best strategy to promote peripheral nerve regeneration with improved anatomy and function among the three different strategies.

Usage: Immunostaining (1:200)

Related Products: NGFr (mu p75) Rabbit Polyclonal (Cat. #AB-N01)