References

Northrop LE, Polston EK, Erskine MS (2010) Noradrenergic nuclei that receive sensory input during mating and project to the ventromedial hypothalamus play a role in mating-induced pseudopregnancy in the female rat. J Neuroendocrinol 22(10):1061-1071. doi: 10.1111/j.1365-2826.2010.02049.x

Summary: Maintenance of pregnancy or pseudopregnancy in rats is maintained by bicircadian prolactin surges induced by vaginal-cervical stimulation. In order to test the hypothesis that medullary noradrenergic cell groups are involved in this process the authors infused rats with either 2 ng or 60 ng anti-DBH-SAP (Cat. #IT-03) into the ventrolateral division of the ventromedial hypothalamus (VMHv1) and the posterodorsal medial amygdala. Mouse IgG-Sap (Cat. #IT-18) was used as a control. The data confirm that noradrenergic neurons are involved in the maintenance of pregnancy or pseudopregnancy.

Related Products: Anti-DBH-SAP (Cat. #IT-03), Mouse IgG-SAP (Cat. #IT-18)

Ariyama H, Kono N, Matsuda S, Inoue T, Arai H (2010) Decrease in membrane phospholipid unsaturation induces unfolded protein response. J Biol Chem 285(29):22027-22035. doi: 10.1074/jbc.M110.126870 PMID: 20489212

Summary: Properties of the cell membrane can be influenced by the degree of fatty acid unsaturation in membrane phospholipids. Alteration of this unsaturation has been implicated in many disease states. Using an anti-SCD1 antibody (Cat. #AB-259) to visualize SCD-1 levels by western blot, the authors determined that there are several genetic factors that affect the level of saturated fatty acid in systems modulating insulin resistance, type 2 diabetes, and cardiovascular disease.

Related Products: SCD-1 Mouse Monoclonal (Cat. #AB-259)

Caudle R, King C, Nolan T, Suckow S, Vierck C, Neubert J (2010) Central sensitization in the trigeminal nucleus caudalis produced by a conjugate of substance P and the A subunit of cholera toxin. J Pain 11:838-846. doi: 10.1016/j.jpain.2010.05.007

Objective: To understand central sensitization.

Summary: Findings suggest that central sensitization leads to activation of an endogenous opioid system. Intracisternal administration of SP-CTA is a useful model for studying central sensitization as a disease process without having to induce a peripheral injury.

Usage: SP-CTA was diluted in PBS and injected in a volume of 10 μl (rats) or 1 μl (mice) over the course of three minutes.

Related Products: SP-CTA (Cat. #IT-39)

Lecourtier L, de Vasconcelos AP, Leroux E, Cosquer B, Geiger K, Lithfous S, Cassel JC (2011) Septohippocampal pathways contribute to system consolidation of a spatial memory: Sequential implication of gabaergic and cholinergic neurons. Hippocampus 21(12):1277-1289. doi: 10.1002/hipo.20837

Summary: Few studies have examined the role of GABAergic septohippocampal projections in memory consolidation. The authors administered 192-IgG-SAP (400 ng; Cat. #IT-01) and/or orexin-SAP (70 ng; Cat. #IT-20) to the medial septum/vertical limb of the diagonal band of Broca of rats. Spatial memory tests were then administered over several weeks. The data indicate that both GABAergic and cholinergic septohippocampal systems contribute to memory stabilization, possibly in a sequential manner.

Related Products: 192-IgG-SAP (Cat. #IT-01), Orexin-B-SAP (Cat. #IT-20)

Gulino R, Perciavalle V, Gulisan M (2010) Featured Article: Role of cell fate determinants in a model of spinal cord neurotoxic lesion induced by cholera toxin b-saporin. Targeting Trends 11(3)

Related Products: CTB-SAP (Cat. #IT-14)

Read the featured article in Targeting Trends.

See Also:

• Gulino R et al. Expression of cell fate determinants and plastic changes after neurotoxic lesion of adult mice spinal cord by cholera toxin-B saporin. Eur J Neurosci 31(8):1423-1434, 2010.

Lappi DA, Ancheta LR, Nowak RA (2010) Featured Article: Internalization of basigin-2, a poor prognosis marker, by a monoclonal antibody. Targeting Trends 11(3) PMID: 0

Related Products: Antibody to Basigin (Cat. #AB-42), Anti-Basigin2-SAP (Cat. #IT-54)

Read the featured article in Targeting Trends.

Carvalho AF, Reyes AR, Sterling RC, Unterwald E, Van Bockstaele EJ (2010) Contribution of limbic norepinephrine to cannabinoid-induced aversion. Psychopharmacology (Berl) 211(4):479-491. doi: 10.1007/s00213-010-1923-7

Summary: The authors used bilateral injections of anti-DBH-SAP (Cat. #IT-03) into the nucleus accumbens and the bed nucleus of the stria terminalis to investigate the role of neuroepinephrine in cannabinoid-induced aversion and anxiety. Lesioned animals received bilateral 52.5 ng-injections of anti-DBH-SAP into the nucleus accumbens or 63 ng into the bed nucleus of the stria terminalis. Saporin (Cat. #PR-01) was used as a control. Lesioned animals displayed reversed aversive behavior, but no change in anxiety-like behavior.

Related Products: Anti-DBH-SAP (Cat. #IT-03), Saporin (Cat. #PR-01)

Berson DM, Castrucci AM, Provencio I (2010) Morphology and mosaics of melanopsin-expressing retinal ganglion cell types in mice. J Comp Neurol 518(13):2405-2422. doi: 10.1002/cne.22381 PMID: 20503419

Objective: To provide a fuller description of murine cell types expressing melanopsin, their contribution to the plexuses of melanopsin dendrites, and mosaics formed by each type.

Summary: M1 cells, corresponding to the originally described ganglion-cell photoreceptors, occupy the ganglion cell or inner nuclear layers. M2 cells ramify in the inner third of the IPL. Rare bistratified cells deploy terminal dendrites within both melanopsin-immunoreactive plexuses within the ON sublayer.

Usage: Immunohistochemistry: The tissue was incubated for 24 hr at 4°C in unpurified primary antiserum diluted 1:2500. Immunoperoxidase labeling of flat mount tissue was incubated for 3 days in the unpurified primary antiserum diluted 1:2500.

Related Products: Melanopsin Rabbit Polyclonal (Cat. #AB-N38)

de Carvalho D, Bicego KC, de Castro OW, da Silva GS, Garcia-Cairasco N, Gargaglioni LH (2010) Role of neurokinin-1 expressing neurons in the locus coeruleus on ventilatory and cardiovascular responses to hypercapnia. Respir Physiol Neurobiol 172(1-2):24-31. doi: 10.1016/j.resp.2010.04.016

Summary: NK-1 receptors (NK1R) play an important role in cardiorespiratory responses to hypercapnia. In order to paint a clearer picture of the systems involved the authors injected 0.4 µl of 2 µM SP-SAP (Cat. #IT-07) into the locus coeruleus (LC) of rats. Mouse IgG-SAP (Cat. #IT-18) was used as a control. The data suggest that several subpopulations of neurons express NK1R in the LC, and that these subpopulations play different roles in the modulation of cardiorespiratory reponses to hypercapnia.

Related Products: SP-SAP (Cat. #IT-07), Mouse IgG-SAP (Cat. #IT-18)

Sugiura A, Ohtori S, Yamashita M, Yamauchi K, Inoue G, Suzuki M, Norimoto M, Orita S, Eguchi Y, Kuniyoshi K, Ochiai N, Kishida S, Takaso M, Aoki Y, Ishikawa T, Arai G, Miyagi M, Kamoda H, Nakamura J, Takahashi K (2010) Effect of applying p75NTR saporin to a punctured intervertebral disc on calcitonin gene-related peptide expression in rat dorsal root ganglion neurons. J Orthop Sci 15(3):407-413. doi: 10.1007/s00776-010-1469-x

Summary: Lumbar intervertebral discs are suspected to be a source of low back pain, in part because of the innervation of these discs by neurons containing substance P and CGRP receptors. Rats received 2.5 µg of 192-IgG-SAP (Cat. #IT-01) into the L5/6 vertebral disc after the disc was punctured. While half of the dorsal root ganglion neurons innervating the disc were positive for CGRP post-puncture, animals receiving 192-IgG-SAP displayed reduced CGRP expression, indicating a role for the p75 receptor in discogenic pain.

Related Products: 192-IgG-SAP (Cat. #IT-01)