References

Koenig J, Lecourtier L, Cosquer B, Pereira PM, Cassel J (2011) Spatial memory alterations by activation of septal 5HT(1A) receptors: no implication of cholinergic septohippocampal neurons. Psychopharmacology (Berl) 214(2):437-454. doi: 10.1007/s00213-010-2049-7

Summary: These experiments examined what effect damaged cholinergic neurons would have on memory deficits induced by the 5-HT1A/5-HT7 receptor agonist 8-OH-DPAT. Rats received 0.4 µg injections of 192-IgG-SAP (Cat. #IT-01) into the medial septum, delivered through an infusion device. Through use of a water maze test, the authors show that several neuronal populations are involved in processing hippocampal information, and non-cholinergic neurons in this region may be more important than the cholinergic ones for memory processing.

Related Products: 192-IgG-SAP (Cat. #IT-01)

Lujan HL, DiCarlo SE (2010) Featured Article: Targeted ablation of sympathetic neurons reduces ventricular arrhythmias and autonomic dysreflexia. Targeting Trends 11(4)

Related Products: CTB-SAP (Cat. #IT-14)

Read the featured article in Targeting Trends.

See Also:

• Lujan HL et al. Targeted Ablation of Cardiac Sympathetic Neurons Reduces the Susceptibility to Ischemia-Induced Sustained Ventricular Tachycardia in Conscious Rats. Am J Physiol Heart Circ Physiol 298:H1330-H1339, 2010.
• Lujan HL et al. Targeted Ablation of Mesenteric Projecting Sympathetic Neurons Reduces the Hemodynamic Response to Pain in Conscious Spinal Cord Transected Rats. Am J Physiol Regul Integr Comp Physiol 298(5):R1358-1365, 2010.

Do MTH, Yau K (2010) Intrinsically photosensitive retinal ganglion cells. Physiol Rev 90(4):1547-1581. doi: 10.1152/physrev.00013.2010

Summary: This review presents recent data that has established the importance of intrinsically photosensitive retinal ganglion cells (ipRPG) in nonimage visual functions. The use of melanopsin-SAP (Cat. #IT-44) in both mice and rats is discussed. It is of note that depletion of ipRPG’s using melanopsin-SAP resulted in deficits in communication to nonimage regions of the brain, but image vision appeared normal.

Related Products: Melanopsin-SAP (Cat. #IT-44)

Lyons AM, Thiele TE (2010) Neuropeptide Y conjugated to saporin alters anxiety-like behavior when injected into the central nucleus of the amygdala or basomedial hypothalamus in BALB/cJ mice. Peptides 31(12):2193-2199. doi: 10.1016/j.peptides.2010.09.009

Summary: Neuropeptide Y (NPY) in the hypothalamus is known to modulate feeding behavior. In this work the authors used bilateral 48 ng injections of NPY-SAP (Cat. #IT-28) into the central amygdala or basomedial hypothalamus (BMH) of rats to investigate the role of NPY in anxiety. Blank-SAP (Cat. #IT-21) was used as a control. Injections into the amygdala increased anxiety-like behavior, while injections into the BMH reduced anxiety-like behavior. BMH injections also initiated an increase of NPY-1 receptor expression in the basolateral nuclei of the amygdala.

Related Products: NPY-SAP (Cat. #IT-28), Blank-SAP (Cat. #IT-21)

Quadros EV, Nakayama Y, Sequeira JM (2010) Targeted delivery of saporin toxin by monoclonal antibody to the transcobalamin receptor, TCblR/CD320. Mol Cancer Ther 9(11):3033-3040. doi: 10.1158/1535-7163.MCT-10-0513

Summary: Vitamin B12 is necessary for cell proliferation. Cancer cells display an increased expression of TCb1R, the receptor that facilitates the intake of B12. In order to evaluate the potential of using immunotoxins to eliminate cancer cells expressing TCb1R the authors performed a series of in vitro experiments using their monoclonal antibodies plus Mab-ZAP (Cat. #IT-04). The results indicate that this is a viable therapeutic model that causes minimal peripheral damage.

Related Products: Mab-ZAP (Cat. #IT-04)

Di Sebastiano AR, Wilson-Perez HE, Lehman MN, Coolen LM (2011) Lesions of orexin neurons block conditioned place preference for sexual behavior in male rats. Horm Behav 59(1):1-8. doi: 10.1016/j.yhbeh.2010.09.006

Summary: The neuropeptide orexin is important in the feedback mechanisms of food intake and drugs of abuse. This work investigates the role of orexin in sexual reward in male rats. Two 200 ng bilateral hypothalamic injections of orexin-SAP (Cat. #IT-20) were made into each hemisphere. Blank-SAP (Cat. #IT-21) was used as a control. Although it was shown orexin neurons are activated by sexual reward cures, the data suggest that orexin is not essential for sexual performance and motivation.

Related Products: Orexin-B-SAP (Cat. #IT-20), Blank-SAP (Cat. #IT-21)

Wang Y, Mu X, Liu Y, Zhang X, Wu A, Yue Y (2011) NK-1-receptor-mediated lesion of spinal post-synaptic dorsal column neurons might improve intractable visceral pain of cancer origin. Med Hypotheses 76(1):102-104. doi: 10.1016/j.mehy.2010.08.042

Summary: There is evidence that spinal post-synaptic dorsal column neurons begin to express neurokinin-1 receptors after visceral stimulation. The authors discuss using this expression profile to target SSP-SAP (Cat. #IT-11) to these neurons and eliminate them. This use of ‘molecular neurosurgery’ may be a replacement for traditional neurosurgery for the treatment of cancer-related visceral pain.

Related Products: SSP-SAP (Cat. #IT-11)

Baskin DG, Kim F, Gelling RW, Russell BJ, Schwartz MW, Morton GJ, Simhan HN, Moralejo DH, Blevins JE (2010) A new oxytocin-saporin cytotoxin for lesioning oxytocin-receptive neurons in the rat hindbrain. Endocrinology 151(9):4207-4213. doi: 10.1210/en.2010-0295

Summary: There is evidence to suggest that release of oxytocin in the nucleus tractus solitarius (NTS) of the hindbrain can inhibit food intake by augmenting the cholecystokinin satiety response. In order to add support to this hypothesis the authors used oxytocin-SAP (Cat. #IT-46) to eliminate oxytocin receptive cells in the NTS. Blank-SAP (Cat. #IT-21) was used as a control. 0.5 µl-injections of oxytocin-SAP into the NTS caused reduced satiation effect of CCK-8 and blocked the stimulation of food intake by an oxytocin receptor antagonist.

Related Products: Oxytocin-SAP (Cat. #IT-46), Blank-SAP (Cat. #IT-21)

Kuroda K, Liu H, Kim S, Guo M, Navarro V, Bander NH (2010) Saporin toxin-conjugated monoclonal antibody targeting prostate-specific membrane antigen has potent anticancer activity. Prostate 70(12):1286-1294. doi: 10.1002/pros.21164

Summary: Current treatments for prostate cancer are only moderately effective. In this work the authors examined the cytotoxic efficacy of an anti-prostate-specific membrane antigen (PMSA) antibody conjugated to saporin on PMSA-positive cell lines. hJ591, a humanized anti-PMSA antibody, was biotinylated and combined with streptavidin-ZAP (Cat. #IT-27). The hJ591-streptavidin-ZAP complex was specifically cytotoxic to PMSA-positive cell lines, and had anti-cancer activity in a xenograft model. This work demonstrates the anti-cancer potential of targeting PMSA.

Related Products: Streptavidin-ZAP (Cat. #IT-27)

Joseph EK, Levine JD (2010) Hyperalgesic priming is restricted to isolectin B4-positive nociceptors. Neuroscience 169(1):431-435. doi: 10.1016/j.neuroscience.2010.04.082

Summary: Hyperalgesic priming is an injury that induces a chronic pain state marked by the presence of inflammatory cytokines. The authors evaluated which populations of nociceptors are involved in the priming process. Rats that received 3.2 µg intrathecal injections of IB4-SAP (Cat. #IT-10) failed to establish priming. Acute mechanical hyperalgesia could still be induced, indicating that IB4+ nociceptors are necessary for priming, but a different nociceptor group is involved with nociceptor sensitization.

Related Products: IB4-SAP (Cat. #IT-10)