Goat-ZAP (Cat. #IT-36) is a new secondary conjugate of affinity-purified rabbit anti-goat IgG and the ribosome-inactivating protein, saporin. This product uses affinity-purified rabbit anti-goat IgG to “piggyback” onto YOUR primary goat antibody in order to evaluate the ability of your primary antibody to internalize. Once the conjugate is internalized, saporin breaks away from the targeting […]
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ATS has added three new products to our fluorescent conjugates line. Alexa488-labeled affinity purified NGFr* (Cat. #AB-N01APFLA) FITC-labeled Goat Anti-Rat IgG (Cat. #FL-10) FITC-labeled Donkey Anti-Goat IgG (Cat. #FL-11) *(Cat. #AB-N01AP) These conjugates are excellent tools to verify specific binding to an antigen. By first incubating cells with the primary antibody, and then binding the
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Q: Do conjugated toxins (dermorphin-saporin in particular) exhibit agonist effects? I’ve generated behavioral and tissue time course effects but have not established agonist effects for this conjugated toxin. A: The peptide ligand toxins should exhibit agonist effects. They are constructed purposely to retain complete agonist activity, including for us the most important: internalization. So, for
Potts JT (2007) Featured Article: Nucleus tractus solitarii injections of substance P-conjugated saporin selectively disrupts baroflex-dependent pathways. Targeting Trends 8(2) Related Products: SP-SAP (Cat. #IT-07) Read the featured article in Targeting Trends. See Also: Potts JT et al. Targeted deletion of neurokinin-1 receptor expressing nucleus tractus solitarii neurons precludes somatosensory depression of arterial baroreceptor-heart rate
Substance P-Saporin (SP-SAP) is a drug candidate that eliminates the spinal cord neurons that send the chronic pain signal to the brain. SP-SAP has been tested in a variety of animal models and proven effective in permanently eliminating the perception of chronic pain. ATS has completed extensive preclinical studies, a GLP toxicology study, and a
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Contributed by Jeffrey T. Potts, Ph.D., Department of Biomedical Science, College of Veterinary Medicine, Dalton Cardiovascular Research Center, Columbia, MO 65211 The nucleus tractus solitarii (NTS) receives afferent input from visceral, somatic and cardiopulmonary structures and is the first central site for the processing of these sensory autonomic signals. Modulation of sensory neurotransmission has been well
Momiyama T, Fukazawa Y (2007) D1-like dopamine receptors selectively block P/Q-type calcium channels to reduce glutamate release onto cholinergic basal forebrain neurones of immature rats. J Physiol 580(Pt 1):103-117. doi: 10.1113/jphysiol.2006.125724 PMID: 17234695 Related Products: 192-IgG Mouse Monoclonal, Cy3-labeled (Cat. #AB-N43FL3)
Potts JT, Fong AY, Anguelov PI, Lee S, McGovern D, Grias I (2007) Targeted deletion of neurokinin-1 receptor expressing nucleus tractus solitarii neurons precludes somatosensory depression of arterial baroreceptor-heart rate reflex. Neuroscience 145(3):1168-1181. doi: 10.1016/j.neuroscience.2007.01.001 Summary: Previous work by these authors examined the role of substance P in arterial baroreflex. Here, 1.5 ng bilateral injections
Martinez L, de Lacalle S (2007) Astrocytic reaction to a lesion, under hormonal deprivation. Neurosci Lett 415(2):190-193. doi: 10.1016/j.neulet.2007.01.020 Summary: One effect of estradiol on astrocytes is the mediation of neuronal sprouting. Astrocytes express glial fibrillary acidic protein (GFAP) in response to injury, but estradiol has been shown to repress GFAP expression. Ovariectomized female rats
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Quinlivan M, Chalon S, Vergote J, Henderson J, Katsifis A, Kassiou M, Guilloteau D (2007) Decreased vesicular acetylcholine transporter and alpha(4)beta(2) nicotinic receptor density in the rat brain following 192 IgG-saporin immunolesioning. Neurosci Lett 415(2):97-101. doi: 10.1016/j.neulet.2006.08.065 Summary: The vesicular acetylcholine transporter (VAChT) is a useful imaging target to assess Alzheimer’s disease, since this transporter