Targeting Tools: Goat-ZAP

Goat-ZAP (Cat. #IT-36) is a new secondary conjugate of affinity-purified rabbit anti-goat IgG and the ribosome-inactivating protein, saporin. This product uses affinity-purified rabbit anti-goat IgG to “piggyback” onto YOUR primary goat antibody in order to evaluate the ability of your primary antibody to internalize. Once the conjugate is internalized, saporin breaks away from the targeting agent and inactivates the ribosomes, which causes protein inhibition and, ultimately, cell death.

This product is excellent for determining the specificity of your antibody. When the in vitro results confirm the desired specificity, it is recommended that you order a custom conjugation of your antibody to saporin.

3T3 cells were plated at 1000 cells/90 μl/well in a 96-well plate and incubated overnight. Saporin dilutions were made in cell media, and 10 μl was added to each well. EGFr antibody was diluted in cell media containing, at a final concentration, 100 ng/10 μl of Goat-ZAP, and 10 μl was added to each well. The plates were incubated 72 hours and developed with MTS/PMS. Data was analyzed by Prism (GraphPad).