Goat-ZAP uses your primary goat affinity-purified polyclonal IgG antibody to target and eliminate cells that recognize your primary antibody. This secondary conjugate uses the secondary antibody (affinity-purified rabbit anti-goat IgG) to “piggyback” onto YOUR goat primary antibody. Goat-ZAP can be utilized for screening goat IgG antibodies for internalization and/or their suitability to make potent immunotoxins. When the in vitro results confirm the desired specificity, it is recommended that you order a custom conjugation of your antibody to saporin.
Goat-ZAP is a chemical conjugate of affinity-purified rabbit anti-goat IgG and the ribosome-inactivating protein, saporin. It uses your goat affinity-purified polyclonal antibody to target and eliminate cells. This secondary conjugate is used to evaluate the potential of a primary antibody to internalize.
Goat-ZAP is available individually (Cat. #IT-36) or as a kit (Cat. #KIT-36) which includes Goat-ZAP, Saporin (Cat. #PR-01), Rabbit IgG-SAP (Cat. #IT-35) and reagents for developing a cytotoxicity assay.