Cover Article: Nucleus tractus solitarii injections of substance P-conjugated saporin selectively disrupts baroflex-dependent pathways.

Contributed by Jeffrey T. Potts, Ph.D., Department of Biomedical Science, College of Veterinary Medicine, Dalton Cardiovascular Research Center, Columbia, MO 65211

The nucleus tractus solitarii (NTS) receives afferent input from visceral, somatic and cardiopulmonary structures and is the first central site for the processing of these sensory autonomic signals. Modulation of sensory neurotransmission has been well documented in the NTS. However, the specific role of the neuropeptide substance P (SP) in the sensory neurotransmission in the NTS remains debated. Previous studies have reported that stimulation of somatic and baroreceptor afferents release SP in the NTS and that neural feedback from skeletal muscle depresses arterial baroreflex function by activating local GABAergic NTS circuits. Since baroreflex depression can be prevented by a pharmacology blockade of neurokinin-1 (NK1) receptors, sensory feedback from skeletal muscle may target a local population of NK1 receptor-expressing GABA neurons that selectively inhibit barosensitive NTS neurons.

To address this question, we injected the neurotoxin substance P-saporin (SP-SAP, Cat. #IT-07), that selectively lesions cells expressing NK1 receptors, into the NTS. SP-SAP (1-3 ng in 100 nl) was bilaterally microinjected medial to the solitary tract at the level of the caudal pole of area postrema and rats recovered 5-7 days post injection. Lesions produced a substantial reduction in the number of NK1 receptor-expressing cells in the NTS compared to animals that received either phosphate buffered saline or non-targeted recombinant saporin (SAP) vehicle injections (Fig 1). Importantly, lesions were restricted to the medial and commissural subdivisions of the NTS and extended rostrocaudally from the caudal to the rostral pole of area postrema. The restricted loss of NK1 receptor-expressing cells may have resulted from the relatively low amount of toxin injected combined with its relatively high molecular weight (33kDa). Loss of NK1 cells was accompanied by a significant reduction in the number of GABA neurons in the same region of the NTS (Fig 2). Cellular toxicity was not responsible for cell loss since no group differences were found in the total number of NTS cells when tissue was stained using Neutral Red.

SP-SAP injections reduced baseline baroreflex function by 70% in treated animals. Despite the reduction in baroreflex sensitivity, SP-SAP also abolished the depressive effect of somatic afferent activation on arterial baroreflex function (Fig 3). In sharp contrast, arterial chemoreflex responses (that are transmitted by chemosensitive carotid body afferents of the glossopharyngeal nerve to similar regions of the NTS) were virtually unaltered by SP-SAP treatment.

Taken together, our results suggest that a restricted population of NK1 receptor-expressing GABA neurons participate in arterial baroreceptor signaling in the NTS. The ability of the SP-SAP toxin to selectively target and lesion NK1 receptor-expressing cells within a very limited region has provided a means to further investigate the role of SP on sensory transmission in the NTS.

Figure 1. NTS lesioning of NK1 receptor-expressing cells.
Panel A and C: Cross-sectional image of rat hindbrain containing the NTS at low magnification (4x). NK1 immunoreactivity was highly expressed on cell soma and fibers in regions medial to the solitary tract (ts) of rats treated with non-targeted SAP toxin. In addition, neurons in the dorsal motor nucleus of the vagus also expressed intense NK1 immunoreactivity. In contrast, NK1 immunoreactivity in the NTS was virtually undetectable in rats receiving SP-SAP.
Panel B and D: High magnification (40X) of NTS region medial of the solitary tract (region of white boxes). The number of NK1-positive soma and fibers are clearly reduced by SP-SAP. Arrow indicates a surviving NK1 receptor-expressing cell and arrowheads indicated spared NK1 fibers.
Figure 2. Immunohistochemical labeling of GAD67 in the NTS using monoclonal mouse anti-GAD67 (MAB5406, Chemicon) and visualized using nickel-enhanced diaminobenzidine.
Upper panels show low and high magnification of GAD67 immunoreactivity medial to the solitary tract (ts) in a SAP-treated rat. Lower panels show similar images obtained from a SP-SAP-treated rat. Note: reduction in GAD67 positive soma (arrows) between SAP- and SP-SAP-treated animals.
Right panel shows group data (n=3 per group) illustrating a significant reduction in GAD67 immunoreactivity following SP-SAP treatment.
Figure 3.Effect of SP-SAP on somatosensory-evoked depression of arterial baroreflex control of heart rate.
Panel A: Graded arterial baroreflex bradycardia produced before (baseline) and during activation of somatic afferents (somatic stim). Somatic stimulation blunted the peak bradycardic response in SAP, but not SP-SAP, treated animals.
Panel B: Group data showing that somatic stimulation failed to blunt the reflex bradycardia in SP-SAP treated animals.

Acknowledgements – NK1 images were obtained by David McGovern, Research Assistant in the Potts Lab using an Olympus BX51-DSU microscope equipped with 4X and 40X objectives and a spinning disk confocal unit. Each image was acquired using Neurolucida software (confocal module) and a Z-axis encoded Ludl stage. Final images are maximal intensity Z-projections created using Image J software (NIH) and visualized using Cy3 secondary antibody. Potts lab is supported by NIH HL059167.