Q: I have a few questions about the Alexa488-labeled affinity-purified NGFr antibody (Cat. #AB-N43-FLA). Is it specific to extracellular p75? Can you use it on live cells? Does it work on fixed cells? Does it cause activation of the p75 receptor (i.e., result in apoptosis or changes in axon outgrowth in neuronal cells)? A: This […]
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Q: Is there is any indication that intrastriatal administration of 192-IgG-SAP (Cat. #IT-01) will lesion the cholinergic neurons of the striatum. My sense from reviewing the literature is that these cholinergic neurons are not susceptible to the toxin, but I thought I’d ask to see if you had any information / experience regarding this point.
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Q: Your targeted toxin data sheet gives the following instruction for disposal: “Care in disposal is mandatory; autoclaving or exposure to 1 M sodium hydroxide will inactivate the material. All labware that comes into contact with this material should be likewise treated.” I am wondering if I can deactivate saporin by using 10% bleach or
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In 1997, SP-SAP (a chemical conjugate of Substance P and the ribosome-inactivating protein saporin) was created to eliminate Substance P receptor (SPR) neurons that propagate the signal for chronic pain. ATS has successfully completed preclinical studies, safety tests in animal models and a GLP toxicology study in rat, funded both internally and by the National
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Shen FM, Wang J, Ni CR, Yu JG, Wang WZ, Su DF (2007) Ketanserin-induced baroreflex enhancement in spontaneously hypertensive rats depends on central 5-HT(2A) receptors. Clin Exp Pharmacol Physiol 34:702-707. doi: 10.1111/j.1440-1681.2007.04626.x Summary: Ketanserin is an anytihypertensive drug that effectively lowers blood pressure, decreases blood pressure variability, and enhances blood pressure response in spontaneously hypertensive
Rahman W, Sikander S, Suzuki R, Hunt SP, Dickenson AH (2007) Superficial NK1 expressing spinal dorsal horn neurones modulate inhibitory neurotransmission mediated by spinal GABA(A) receptors. Neurosci Lett 419:278-283. doi: 10.1016/j.neulet.2007.04.039 Summary: It has been shown that elimination of lamina 1 NK1 receptor-expressing neurons affects pain behaviors. The authors investigated whether eliminating these neurons would
Gibbs RB (2007) Estradiol enhances DMP acquisition via a mechanism not mediated by turning strategy but which requires intact basal forebrain cholinergic projections. Horm Behav 52:352-359. doi: 10.1016/j.yhbeh.2007.05.011 Summary: Estradiol appears to enhance cholinergic projections to the hippocampus and frontal cortex as shown by tests of response patterns and strategy in rats. The author tested
Wiley RG, Kline IV RH, Vierck Jr CJ (2007) Anti-nociceptive effects of selectively destroying substance P receptor-expressing dorsal horn neurons using [Sar(9),Met(O(2))(11)]-substance P-saporin: Behavioral and anatomical analyses. Neuroscience 146:1333-1345. doi: 10.1016/j.neuroscience.2007.01.066 Summary: While lumbar injections of SP-SAP (Cat. #IT-07) produce specific lesions, use of this targeted conjugate in the forebrain has been problematic. The authors
Hanganu IL, Staiger JF, Ben-Ari Y, Khazipov R (2007) Cholinergic modulation of spindle bursts in the neonatal rat visual cortex in vivo. J Neurosci 27:5694-5705. doi: 10.1523/JNEUROSCI.5233-06.2007 Summary: The authors investigated the relationship between cholinergic drive and spindle burst oscillation driven by retinal waves. 0.5 µl of 0.2-µg/µl 192-IgG-SAP (Cat. #IT-01) was injected into both
Cholinergic modulation of spindle bursts in the neonatal rat visual cortex in vivo. Read More »
Gómez-Pinilla PJ, Pozo MJ, Camello PJ (2007) Aging impairs neurogenic contraction in guinea pig urinary bladder: role of oxidative stress and melatonin. Am J Physiol Regul Integr Comp Physiol 293(2):R793-803. doi: 10.1152/ajpregu.00034.2007 PMID: 17522125 Related Products: Substance P Rabbit Polyclonal (Cat. #AB-N10)