Anti-dopamine beta-hydroxylase-saporin (anti-DBH-SAP, Cat. #IT-03) is a highly specific noradrenergic lesioning agent. It specifically targets rat cells that express dopamine beta-hydroxylase and is also reported to react with mouse protein.1 This vesicular enzyme is exposed to the exterior milieu upon release of noradrenaline and thus allows these cells to be targeted with saporin. The specificity […]
Targeting Tools: Anti-DBH-SAP Read More »
Q: Is there a saporin derivative available that selectively destroys all or some hippocampal neurons? A: Assuming that you mean to eliminate cell bodies from the hippocampus, rather than just projections, a neat paper by Martin et al. describes the use of SSP-SAP (Cat #IT-11) to do this. NPY-SAP (Cat. #IT-28) could also be interesting.
Destroy Hippocampal Neurons Read More »
Q: When we contacted you to find out more about having a custom saporin conjugation performed with our primary antibody, you recommended that we use the ATS secondary conjugate system to determine that our antibody was specific to the population we want to eliminate. We looked more at the website, and it seems that we
Custom Mouse IgM Conjugate and Controls Read More »
Q: I am interested in your product GAT1-SAP (Cat. #IT-32). Before ordering, I would like to know how long it takes until the toxin produces a lesion; Is seven days enough? A: The usual time-course for saporin toxins is that behavioral effects start appearing at four days and usually plateau at seven days. However, keep
Time Course for Toxins Read More »
Wagle M, Mathur P, Guo S (2011) Corticotropin-releasing factor critical for zebrafish camouflage behavior is regulated by light and sensitive to ethanol. J Neurosci 31(1):214-224. doi: 10.1523/JNEUROSCI.3339-10.2011 PMID: 21209207 Objective: To explore neural circuit assembly and function using the hardwired camouflage response of zebrafish. Summary: Both light exposure and ethanol affect the camouflage response. Understanding
Goehler LE, Gaykema RPA (2011) Featured Article: Targeted lesion of caudal brainstem catecholamine neurons reveals their role in symptoms of fatigue. Targeting Trends 12(1) Related Products: Anti-DBH-SAP (Cat. #IT-03), Mouse IgG-SAP (Cat. #IT-18) Read the featured article in Targeting Trends.
Contributed by Lisa E. Goehler and Ronald P.A. Gaykema Center for the Study of Complementary and Alternative Therapies, University of Virginia School of Nursing, Charlottesville, VA 22908 Fatigue, experienced as reduced motivation and motor activity, is common in acute and chronic disease, including heart disease, cancer and cancer chemotherapy. Fatigue is part of a constellation
Read the 2010 Newsletters and recent scientific references 2010, issue 1: 2010, issue 2: 2010, issue 3: 2010, issue 4:
Targeting Trends 2010 Newsletter Read More »
Zhang L, Hadley GA (2010) Application of anti-CD103 immunotoxin for saving islet allograft in context of transplantation. Chin Med J (Engl) 123(24):3644-51. Summary: This work investigates whether depletion of CD103-positive cells protects transplanted islets from host-immune cell attack. Diabetes was induced in mice, followed by an islet transplant. Anti-CD103-SAP (Cat. #IT-50) was administered via i.p.
Yang MY, Chaudhary A, Seaman S, Dunty J, Stevens J, Elzarrad MK, Frankel AE, St Croix B (2011) The cell surface structure of tumor endothelial marker 8 (TEM8) is regulated by the actin cytoskeleton. Biochim Biophys Acta 1813(1):39-49. doi: 10.1016/j.bbamcr.2010.11.013 Summary: Tumor endothelial marker 8 (TEM8) is a cell surface protein that is up-regulated on