Q: We are setting up some experiments in rat where we’d use your 192-IgG-SAP (Cat. #IT-01) in cytotoxicity assays. How much material do we need to order? A: You can find protocols for calculating the amount of material needed for a cytotoxicity assay and protocols for the assay and interpretation of results on our website. […]
Cytotoxicity Assay Protocols Read More »
Q: I am using your Bombesin-SAP (Cat. #IT-40) to kill GRP-receptor in mouse brain. I have a plan to inject it by iontophoresis. Do you know which charge dose Bombesin-SAP and Blank-SAP (Cat. #IT-21) have; plus charge or negative charge? A: Both of these products will have a negative charge, though you may have to
What charge does Bombesin-SAP and Blank-SAP have Read More »
Q: We’ve been using Mab-ZAP to test our primary mouse monoclonal antibody in cytotoxicity assay. Relative to the Primary mAb-Mab-ZAP complex, the Mab-ZAP alone has quite a bit of activity (40-60% growth inhibition) at the recommended concentrations used (45 or 100 ng/well) or even half that dose. Even though we get a dose response, the
Higher concentration and less cytotoxicity Read More »
Q: I would like to know if the secondary antibody used to prepare Mab-ZAP (Cat. #IT-04) reagent binds to heavy chain of mIgG’s (only) or if it recognizes light chains as well? A: Mab-ZAP will recognize whole IgG and will bind to both the heavy and light chain. Related Product: Mab-ZAP (Cat. #IT-04)
Does Mab-ZAP bind heavy and light chain? Read More »
Luo T, Leung LS (2011) Involvement of tuberomamillary histaminergic neurons in isoflurane anesthesia. Anesthesiology 115(1):36-43. doi: 10.1097/ALN.0b013e3182207655 Summary: Although previous studies indicate that histaminergic neurotransmission may mediate reaction to general anesthesia, it is not clear whether the histominergic tuberomammilary nucleus (TMN) is involved. Rats received 250-ng infusions of orexin-SAP (Cat. #IT-20) into the TMN after
Involvement of tuberomamillary histaminergic neurons in isoflurane anesthesia. Read More »
Gelfo F, Tirassa P, De Bartolo P, Caltagirone C, Petrosini L, Angelucci F (2011) Brain and serum levels of nerve growth factor in a rat model of Alzheimer’s disease. J Alzheimers Dis 25(2):213-217. doi: 10.3233/JAD-2011-110047 PMID: 21368378 Objective: Using nerve growth factor (NGF) as a marker of disease progression in Alzheimer’s disease using cholinergic depletion
Brain and serum levels of nerve growth factor in a rat model of Alzheimer’s disease Read More »
Mitra N, Banda K, Altheide TK, Schaffer L, Johnson-Pais TL, Beuten J, Leach RJ, Angata T, Varki N, Varki A (2011) SIGLEC12, a human-specific segregating (pseudo)gene, encodes a signaling molecule expressed in prostate carcinomas. J Biol Chem 286(26):23003-23011. doi: 10.1074/jbc.M111.244152 Summary: Siglec 12 (sialic acid-binding immunoglobulin-like lectin 12) is a sugar molecule that has mutated
Radley JJ, Sawchenko PE (2011) A common substrate for prefrontal and hippocampal inhibition of the neuroendocrine stress response. J Neurosci 31(26):9683-95. doi: 10.1523/JNEUROSCI.6040-10.2011 Summary: In order to better understand how response to emotional stress is regulated, the authors injected 114 ng of GAT-1-SAP (Cat. #IT-32) into each side of the anterior bed nucleus of the
St Peters M, Demeter E, Lustig C, Bruno JP, Sarter M (2011) Enhanced control of attention by stimulating mesolimbic-corticopetal cholinergic circuitry. J Neurosci 31(26):9760-9771. doi: 10.1523/JNEUROSCI.1902-11.2011 Summary: Motivation and attention interact to preserve cognitive performance under challenging conditions. In order to better define the circuitry connecting these two processes, the authors lesioned the prefrontal cortex
Polito L, Bortolotti M, Pedrazzi M, Bolognesi A (2011) Immunotoxins and other conjugates containing saporin-s6 for cancer therapy. Toxins (Basel) 3(6):697-720. doi: 10.3390/toxins3060697 PMID: 22069735
Immunotoxins and other conjugates containing saporin-s6 for cancer therapy. Read More »